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Mcm3 (NM_008563) Mouse Untagged Clone
CAT＃: MC205531
Mcm3 (untagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), (10ug)
CNY 5,952.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AL033361; C80350; Mcmd; P1; P1-MCM3; p1.m |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC031700
GCGACATGGCGGGCACAGTAGTGCTGGATGATGTGGAGCTGCGGGAAGCGCAGAGAGACTACTTGGACTT CCTGGATGACGAGGAGGACCAAGGCATTTACCAGAACAAGGTTCGGGAACTGATCAGTGACAACCAGTAT CGGCTGATTGTCAGCGTGAATGACCTGCGCAGGAAGAATGAAAAGAGGGCTAACCGCCTCCTGAACAATG CGTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTGAAGGATTTCGTGGCCTCCATTGACGCCACCTACGC CAAGCAGTATGAGGAGTTCTACATAGGATTGGAAGGCAGCTTTGGCTCAAAGCACGTTTCTCCCCGGACT CTCACTTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAAGGCATTGTTACTAAATGCTCTCTGGTTCGCC CCAAAGTCGTTCGCAGTGTTCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGCTACTCTGACCT CACCACCCTAGTGGCTTTCCCATCCAGTTCTGTTTATCCCACAAAGGATGAGGAGAACAACCCCCTGGAG ACAGAGTATGGCCTGTCTGTCTACAAGGACCACCAGACCATCACCATCCAGGAGATGCCAGAGAAGGCCC CTGCTGGTCAGCTCCCTCGCTCCGTGGACGTCATTCTAGACGATGACCTGGTGGATAAAGTGAAGCCTGG TGACAGAATACAGGTGGTGGGGACCTACCGCTGCCTTCCAGGGAAGAAGGGCTGCTACACCTCGGGGACC TTCAGGACCGTCCTGATTGCCTGTAATGTGAAGCAGATGAGTAAGGATATTCAGCCTGCATTCTCTGCGG ACGATATAGCCAAGATCAAGAAGTTCAGCAAAACTCGTTCCAAGGATGTCTTTGAGCAGCTGGCCCGGTC GTTAGCCCCGAGCATCCATGGGCATGACTATGTCAAGAAGGCAATCCTCTGCTTGCTCCTGGGAGGGGTG GAGCGGGAACTTGAAAACGGCAGCCACATCCGTGGAGACATCAATATTCTTCTAATAGGTGACCCCTCCG TTGCCAAGTCCCAGCTTCTGCGGTACGTGCTTTGCACGGCGCCCAGGGCCATTCCCACCACGGGACGGGG CTCCTCTGGAGTGGGTCTCACAGCTGCTGTCACTACAGACCAGGAAACAGGGGAGCGCCGCCTGGAAGCT GGTGCTATGGTCCTGGCTGACCGTGGAGTGGTTTGCATTGATGAGTTTGACAAGATGTCTGACATGGACC GCACAGCCATCCACGAGGTGATGGAGCAGGGTCGAGTGACCATTGCAAAGGCTGGCATCCATGCCCGACT CAATGCCCGCTGCAGTGTTTTGGCAGCTGCCAATCCAGTCTATGGCAGGTATGATCAGTATAAGACCCCC ATGGAGAACATTGGGCTGCAGGACTCACTGCTGTCTAGATTTGACCTGCTCTTCATCATGCTGGATCAGA TGGATCCTGAACAGGATCGGGAGATCTCAGACCACGTCCTTAGGATGCACCAATACAGGGCCCCGGGGGA GCAGGACGGTGATGCTTTGCCATTGGGTAGTTCAGTGGATATCCTGGCCACAGACGATCCTGACTTTACC CAGGATGACCAGCAAGATACCCGCATTTATGAGAAGCATGACAGCCTTCTACATGGGACCAAGAAGAAAA AGGAGAAGATGGTGAGCGCAGCTTTTATGAAGAAGTACATTCACGTGGCCAAAATTATCAAGCCCACCCT GACACAGGAGTCAGCAGCCTACATTGCAGAAGAATACTCACGCCTGCGCAGCCAGGACAGTATGAGCTCA GACACTGCCCGGACATCTCCAGTTACAGCACGGACACTGGAAACTCTGATTCGACTGGCCACGGCCCATG CGAAGGCCCGGATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCCGTGGAGCTGGTTCAGTATGC TTATTTCAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGGCAAGTGAGGATGAATCAGACCTAGAA GATGAAGAGGAAAAGAGCCAGGAAGACACTGAGCAGAAGCGGAAAAGGAGGAAGACTCATGCCAAGGATG GAGAGTCCTATGACCCTTACGACTTCAGTGAAGCAGAGACGCAGATGCCTCAAGTGCACACCCCAAAGAC TGACGATTCCCAAGAGAAGACCGATGATTCTCAGGAGACCCAGGACTCCCAGAAAGTGGAACTCAGTGAG CCCAGGCTGAAGGCTTTTAAGGCGGCCCTCTTAGAAGTGTTCCAGGAAGCTCATGAGCAGTCTGTGGGCA TGCTCCACCTCACAGAATCCATCAACCGGAATAGAGAAGAGCCTTTCTCCTCAGAGGAAATCCAGGCTTG CCTGAGCCGGATGCAGGATGATAACCAGGTCATGGTGTCTGAGGGCATCGTCTTCCTTATCTGAAGTTGT TGCTACCAAGTACTGCCCTGTCCCCAGCCTTTGTCTCCCATCCTCCAGTAACTTTTTTAAGGGATGGAAG CTGATGGTGAGATGATCAGGACTTGGTGGAAGCTTTGTGTATGACTTCTGCCTTGGGGCAAGAGCTGGAG GGAGGGCCAGGACTCAGGCAGAAGGCCTGCCTCACAAGCCCAATCCTAAACATGGGGAGTCTTCATGATT TGAGCTGTGTTTCTGGGCACAGCTGTGTCCTGCGTTTGTTACTTGTGTTTTTACCCCCACCCCCAGGCAC TTTAGTCCAGGAAGCTTTGAGGTCTGTTGGGCCAAGAGTGGCTCAGGCAGATGACAAAGTTGGATTGGAG AAAGCTTTCTTTGCGATTAGACTAGTATGTATTGTTGACCTAAATATCCAAGACATTGATACTGAGTAAA TATAACTATTTTGTAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_008563 |
| Insert Size | 2439 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031700, AAH31700 |
| RefSeq Size | 2899 bp |
| RefSeq ORF | 2439 bp |
| Locus ID | 17215 |
| UniProt ID | P25206 |
| Gene Summary | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG215631 | Mcm3 (tGFP-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), (10ug) |
CNY 8,930.00 |
|
| MR215631 | Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3) |
CNY 8,080.00 |
|
| MR215631L3 | Lenti ORF clone of Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3) |
CNY 9,980.00 |
|
| MR215631L4 | Lenti ORF clone of Mcm3 (mGFP-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3) |
CNY 9,980.00 |
