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Nprl2 (NM_018879) Mouse Untagged Clone
CAT＃: MC205833
Nprl2 (untagged) - Mouse nitrogen permease regulator-like 2 (S. cerevisiae) (Nprl2), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2810446G01Rik; G21; NPR2L; Tusc4 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC026548
CTCTTTTTGACAGGGTATTCCAAGGCGTAGAGGCGATCCCCACACGAAAAACAGGGATTACAGACTTGGA TCCAGACAGCGCCATGGGCAGCAGCTGCCGCATCGAATGTATATTCTTCAGCGAGTTCCACCCAACGCTG GGACCCAAAATTACCTATCAGGTCCCTGAGGATTTCATCTCGCGAGAACTGTTTGACACGGTCCAGGTGT ACATCATCACCAAGCCCGAGTTACAGAACAAGCTCATCACTGTCACAGCCATGGAGAAGAAGCTGATTGG CTGCCCCGTGTGCATTGAACACAAGAAGTACAGCCGCAATGCTCTCCTCTTCAACCTGGGCTTTGTGTGT GACGCTCAGGCTAAGACTTGTGCCCTGGAACCCATCGTTAAAAAGCTGGCCGGCTACTTGACCACGCTGG AGCTTGAGAGCAGCTTTGTATCCAACGAGGAGAGCAAGCAGAAGCTGGTGCCCATCATGACCATCCTGCT GGAAGAGCTGAATGCCTCCGGCCGCTGCACTCTGCCCATTGACGAATCCAACACCATCCACTTGAAGGTG ATCGAGCAGCGGCCAGACCCTCCTGTTGCGCAGGAGTATGATGTGCCCGTCTTTACAAAGGACAAGGAGG ATTTCTTCAGCTCGCAGTGGGACCTTACCACACAGCAGATCCTGCCCTACATTGACGGGTTTCGCCATGT CCAGAAGATCTCCGCTGAGGCAGACGTGGAACTCAACCTGGTGCGCATCGCCATTCAGAACCTGCTGTAC TATGGTGTTGTGACACTGGTATCCATCCTCCAGTACTCCAATGTGTATTGCCCAACACCAAAAGTCCAGG ATCTGGTGGATGACAAGTCCCTGCAGGAGGCATGTCTGTCCTACGTCACCAAGCAAGGACACAAAAGGGC CAGTCTCCGAGATGTGTTCCAGCTATACTGCAGCCTGAGCCCTGGTACCACTGTGCGAGACCTCATCGGC CGCCACCCCCAGCAGTTACAACACGTTGATGAAAGGAAGCTGATCCAGTTTGGGCTGATGAAGAACCTTA TCCGGAGACTACAGAAATATCCAGTCCGAGTGTCTCGGGAGGAGCGGAGCCACCCTGCCAGGCTTTACAC GGGCTGTCACAGCTATGATGAGATCTGCTGCAAGACAGGCATGAGCTACCATGAGCTGGACGAGCGACTA GAAAATGACCCCAACATCATCATCTGCTGGAAGTGAGGCTGGCTGGCTGGAGCATGTTACTATGGCTACT TGCCCCTACTAAGGCCTCCTTGGTCCACGGGAGCTAGACAAGTATGGTTGTACATATGGTCTGGAGGGTG AGACTCGGTTTTGTAAATAAAGTTATGTGTTTCATCCTGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_018879 |
| Insert Size | 1143 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026548, AAH26548 |
| RefSeq Size | 1432 bp |
| RefSeq ORF | 1143 bp |
| Locus ID | 56032 |
| UniProt ID | Q9WUE4 |
| Gene Summary | As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205929 | Nprl2 (tGFP-tagged) - Mouse tumor suppressor candidate 4 (Tusc4) |
CNY 3,140.00 |
|
| MR205929 | Nprl2 (Myc-DDK-tagged) - Mouse nitrogen permease regulator-like 2 (S. cerevisiae) (Nprl2) |
CNY 3,656.00 |
|
| MR205929L3 | Lenti ORF clone of Nprl2 (Myc-DDK-tagged) - Mouse nitrogen permease regulator-like 2 (S. cerevisiae) (Nprl2) |
CNY 4,750.00 |
|
| MR205929L4 | Lenti ORF clone of Nprl2 (mGFP-tagged) - Mouse nitrogen permease regulator-like 2 (S. cerevisiae) (Nprl2) |
CNY 4,750.00 |
