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Zfp281 (BC062153) Mouse Untagged Clone
CAT＃: MC205914
Zfp281 (untagged) - Mouse zinc finger protein 281 (cDNA clone MGC:70044 IMAGE:6335572), (10ug)
CNY 6,552.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC7737, MGC70044 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC062153
GCGGCTGCGCAGAAGCGGGGAGGCTTTTAATTCCATTGTGGAGAGGACGGCGTTATTTTTATTAACTGGA GGCGACGGCGGCTGCGGCGGCGGCGGAACCCCCAGGCCTCCTCCGGGGTATGAAAATCGGCAGCGGGTTC CTTAGCGGCGGCGGCGGTCCCAGCAGTAGCGGTGGTAGCGGCTCCGGCGGCAGCTCCGGCAGCGCCAGCG GCGGCAGCGGCGGCGGCAGGAGAGCAGAGATGGAGCCCACCTTCCCCCAGAGTATGGTTATGTTCAACCA CCGGCTTCCCCCGGTCACCAGCTTCACCCGGCCGGCGGGGACGGCCGCCCCTCCCCCGCAGTGCGTGTTA TCCTCCTCTACCTCCGCAGCCCCGGCCGCTGAGCCCCCCCCTCCGCCAGCCCCGGACATGACTTTCAAGA AGGAGCCGGCGGCGTCAGCCGCGGCCTTCCCTTCGCAGAGGACCTCCTGGGGATTCTTGCAGTCCTTGGT GAGCATCAAGCAGGAGAAACCTGCGGATCCCGAGGAGCAGCCGTCCCACCACCACCATCACCACCACCAC TATGGGGGGCTGTTCGCTGGGGCTGAAGAGCGATCACCAGGCCTAGGAGGAGGGGAAGGGGGGAGCCACG GCGTCATCCAAGACCTCAGTCTTCTGCACCAGCATGCCCAGCAGCAACCGGCCCAGCACCACCGCGATGT ATTACTGAGCAGCGGTAGCAGGACTGATGAGCACGGCAACCAGGAGCCAAAGCAGGACGCTAACGTCAAA AAGGCAAAGAGGCCAAAGCCAGAATCTCAGGGAATCAAAGCCAAGAGGAAGCCAAGTGCATCTTCCAAAC CTTTGGTTGGAGAGGGAGAAGGTGCCGTCCTGTCCCCAAGTCAGAAACCTCATATCTGTGATCACTGTAG TGCTGCTTTCAGGAGCTCCTACCACCTGCGGAGACATGTCCTCATCCACACGGGCGAAAGGCCTTTCCAG TGCAGCCAGTGTAGTATGGGTTTCATCCAGAAATACCTACTGCAGAGACACGAGAAAATTCACAGCAGAG AGAAACCCTTTGGGTGCGATCAGTGCAGCATGAAGTTTATTCAGAAGTACCATATGGAGAGACACAAGAG GACACATAGTGGAGAAAAGCCATACAAGTGTGACACTTGCCAACAGTATTTTTCAAGGACTGACAGATTG TTGAAGCACAGGCGCACGTGTGGTGAAGCCATAGCAAAAGGAGCCGCTAGTGCAGAACCTGGGTCATCAA ACCATAACAGTATGGGTAACCTGGCTGTGTTGTCTCAGGGAAATACAAGTTCCTCAAGGAGAAAGTCGAA GTCGAAAAGCATAGCTATTGAAAATAAGGAACACAAGACTGGCAAAACAAATGAATCACAAATGTCAAAC AATATAAACATGCAGAGTTATTCAGTAGAAATGCCTACTGTGTCTACCAGTGGGAGCATAATTGGCACTG GAATAGATGAACTGCAGAAAAGGGTGCCAAAATTGATCTTTAAGAAAGGAAGCAGAAAGAATGCAGATAA AAGCTACCTTAATTTTGTGTCACCGTTACCAGACGTAGTTGGGCAGAAATCCTTGTCTGGTAAGCCAGGT GGCTCACTTGGCATCGTATCGAATAATAGTGTGGAGACCATTAGTCTTCTCCAAAGTACAAGTGGCAAGC AAGGCCCGATAAGTAGTAATTATGATGATGCCATGCAGTTTTCAAAGAAGAGAAGATACCTACCAACTGC CAGCAGCAACAGTGCCTTTTCTATCAATGTAGGACACATGGTCTCCCAGCAGTCAGTCATTCAGTCTGCA GGGGTCAGTGTTTTGGACAATGAGGCGCCATTGTCACTTATTGACTCCTCAGCTCTAAATGCTGAAATTA AGTCTTGTCACGACAAGTCTGGAATTCCTGATGAGGTTTTACAAAGTATTTTGGATCAGTACTCTGGCAA ATCAGAAACACAGAAGGAGGATCCTTTCAATTTAACAGAACCACGAGTGGATTTACACACCTCAGGAGAA CACTCTGAATTGGTTCAAGAAGAAAATTTGAGCCCAGGCACCCAAACACCTTCAAATGATAAAACAAGCA TGTTGCAAGAATACTCCAAATACCTCCAACAGGCTTTCGAAAAATCCACTAATGCAGGTTTTACTCTTGG ACACGGTTTCCAATTTGTCAGCTTGTCTTCACCTCTCCACAACCACACTTTGTTTCCAGAAAAACAGATA TACACTACATCTCCTTTGGAGTGTGGTTTTGGCCAGTCTGTTACCTCAGTGCTGCCATCTTCGTTGCCAA AGCCTCCTTTTGGGATGTTGTTTGGATCTCAACCAGGTCTTTATTTATCTGCTTTGGATGCTACACATCA GCAGTTGACACCTTCCCAGGAGCTGGATGATCTGATAGATTCTCAGAAGAACTTAGAGACATCATCAGCC TTCCAGTCCTCATCTCAGAAATTGACTAGCCAGAAGGAACAACAGAAAAATTTAGAGTCCTCAACGAGCT TTCAGATTCCATCTCAGGAGTTAGCTAGCCAGATTGATCCTCAGAAAGACATAGAGCCTAGAACAACGTA CCAGATTGAGAACTTTGCACAAGCATTCGGTTCTCAGTTTAAGTCGGGCAGCAGGGTGCCAATGACCTTT ATCACTAACTCTAATGGAGAAGTGGACCATAGAGTAAGGACTTCAGTGTCAGATTTCTCAGGGTATACAA ATATGATGTCTGATGTAAGTGAGCCATGTAGTACAAGAGTAAAGACACCCACCAGCCAGAGTTACAGGTA AGGTCTCGAGAGTGACCAGGCTGGGAGTCTAATGTAATTTTGTTTTATTTTGAGAACACTGCCATTGGAA TGTTTCTACACGATCCTATTAAGAATAATGTAATGCCCTTTCAATGCAACTTTTCATATTTAGTTTATTT TGTTAGTGTGATTTTAGCTCTGTTTGTATTATGATTTTTAATCAAAATCAATAGATTAAAAATAGTTTGA CATTCAAAGTGACAATGTTTAGCAATCAAATTTACATGTATAGATTGTCAGGGAATAGCCCAAAAGTTTT AAATGCAAAAACAAAAAAAATAAAACAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC062153 |
| Insert Size | 2682 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC062153, AAH62153 |
| RefSeq Size | 3155 bp |
| RefSeq ORF | 2682 bp |
| Locus ID | 226442 |
| Gene Summary | Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.[UniProtKB/Swiss-Prot Function] |
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