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Ythdf1 (BC061479) Mouse Untagged Clone
CAT＃: MC205938
Ythdf1 (untagged) - Mouse YTH domain family 1 (cDNA clone MGC:70052 IMAGE:6334535), (10ug)
CNY 4,168.00
货期*
现货
规格
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经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2210410K23Rik; 8030473O16 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC061479
CGGCGCCCCCTCAGCCTGTCCGCTACCTTCCGCTGCTTCCCGCTGTTCCGGCCCGCGTGCCTACAGCTGC CGCCGCGCCCGGCCGCACCCGCGGAGTTGGCGGCGGGGCACGGGGCCGCGCGCGGCGTGACAGGCCCGGC CTCGTGGGAGGCCCGAGCCGGCGGCGCCCGGGTCCCGCGCCGCGCTGTTCATGAAGCATGTCGGCCACCA GCGTGGACCCCCAGAGAACAAAAGGACAAGATAATAAAGTACAAAATGGTTCTTTGCATCAGAAGGATGC AGTTCATGACAATGACTTTGAGCCCTACCTTTCTGGACAGTCCAATCCGAGTAACAGTTACCCCTCGATG AGTGATCCTTACCTGTCCAGTTACTATCCACCATCCATTGGATTTCCTTACTCCCTCAGCGAGGCACCAT GGTCCACTGCAGGGGACCCTCCCATCCCGTATCTCACTACCTATGGACAACTTAGTAATGGAGACCATCA CTTCATGCATGATGCTGTTTTTGGGCAGCCTGGGGGTCTGGGGAACAACATTTACCAGCACAGGTTTAAT TTTTTCCCTGAAAACCCTGCATTCTCAGCATGGGGGACAAGTGGTTCTCAGGGGCAGCAGACTCAGAGCT CAGCCTATGGGAGCAGTTACACTTACCCACCTAGCTCCCTTGGTGGCACAGTTGTTGATGGGCAGACAGG TTTTCACAGTGACTCCCTCAACAAGGCCCCTGGGATGAACAGTCTGGAGCAGGGCATGGTTGGCCTGAAG ATTGGGGATGTTACCACCTCTGCAGTTAAGACGGTGGGTTCAGTTGTCAACAGTGTGGCACTGACTGGTG TCCTTTCTGGCAATGGTGGGACAAATGTAAACATGCCAGTTTCAAAACCAACTTCATGGGCAGCCATTGC TAGCAAGCCTGCAAAACCACAGCCTAAGATGAAAACAAAGAGTGGGCCGATAGTTGGGGGTGCACTGCCT CCTCCCCCTATCAAGCATAACATGGACATTGGTACTTGGGATAACAAGGGCCCTGCTCCAAAGGCCTCCG CCCCCCAGCAGACACCATCCCCCCAGGCTGCCCCACAGCCCCAGCAGGTAGCTCAACCTCTCCCTGTTCA GCCCCCACCTTTGGTCCAGCCACAGTATCAGAGCCCTCAGCAGCCACTTCAACCCCGCTGGGTGGCTCCT CGAAACAGAAATGCAGCATTTGGGCAGAGTGGAGGGGCCAACAGTGACAGTAACTCTGTTGGAAATGCCC AACCTACTTCTGCCCCGAGTGTAGAATCCCACCCTGTCCTGGAGAAACTGAAAGCTGCCCACAGCTATAA CCCTAAAGAGTTCGACTGGAATCTTAAGAGTGGGCGGGTGTTCATCATCAAGAGCTATTCTGAGGACGAC ATCCACCGCTCCATCAAGTACTCCATCTGGTGTAGTACTGAACACGGCAACAAGCGCCTGGACGGCGCCT TCCGCTCCATGAGCAGCAAGGGGCCTGTTTATCTCCTCTTCAGTGTCAATGGGAGTGGACATTTCTGTGG GGTGGCAGAGATGAAGTCCCCTGTGGACTACGGCACCAGCGCTGGGGTCTGGTCTCAGGACAAGTGGAAG GGAAAGTTTGATGTGAAGTGGATTTTTGTGAAGGATGTGCCCAACAACCAGCTGCGGCACATCAGACTGG AGAATAACGACAACAAACCTGTCACAAACTCCCGTGATACACAGGAGGTGCCCTTAGAAAAAGCAAAACA AGTGCTGAAGATTATCGCTTCCTATAAGCACACAACCTCTATCTTTGACGACTTTTCTCATTATGAGAAG CGCCAGGAGGAAGAGGAGGTGGTGCGTAAGGAAAGACAGAATCGAAACAAACAATAAGAACAAGCCAGTT TGTTTTTGGTTAATGGTTGACTTTGAAAACAGAGTTTTAAAGCTGTATGCTTGGTGCTGTCTCCGAGTCA GCTCCAGTGTCGTCCTCGTGCGGGGTTGATTGTTGCATCTTTATCTTTGTAGTTCATTTTTGCCAGATGG ATCTGCATTCATTTGTATTTTTCTATGTATTATAATATTGTAGAACTCACTAATAAAGGAGTATTTTGTT TGTCAGCTTATCAGTCAGATTGACCTAATGCAAAATATAAATATTCTTCAAAACAAAACACCTAATACAT CCCAAAGATATTTTTATTTTGGCAGAAATTATGTTCTTATTCTTATATCTAGCCATTTAATATCCCTATA GGATACTTCATATTTCTTGTCCTCTCCTGCTTTACAGCAAACAAAAAGCCCATTCAGCATGTGCTGTTGA TGTCTCTTCACAGTACTGAAGTGGCTTGCCATTTTACTATCTGTGGTATCCATCATATTGCTGACCTGCC CTTTTGAGTTTCCTACTAGACAAATAACTTTTAGTTTTAAGTGTGCTCACAGCTGTCTATGGGCAAGCTG TCCATAGTTTAGTGCCCACACAGAGCGGTGCTCCAGTTCTCCTTGCCACCGTCTGCCACTGCATGAGGTT TGGGCTCTGTGGCAAGGAACTAGAAGGTGTTTTAAGTCTGGTTCAGCTTTGCGACATTCTTAGTTGTTCG TAGATCATTTTTAGTTGTCCTCTTTCTTCTCTAGAGAATCCTGATGCTGTCTTAAGTTTTTATACCAGTA ACGCTAAGCTTTAATTGTAGGAATCTGATAGTACAGGCAGTGTGATGGATGCTGCTGCCGACTGTAAATT TCACTGTGTGTCTATTTTTTTTTTCTGTTGAATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC061479 |
| Insert Size | 1680 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC061479, AAH61479 |
| RefSeq Size | 2915 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 228994 |
| Gene Summary | Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:30401835). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:30401835). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (By similarity). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory (PubMed:30401835). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation (PubMed:30843071). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (PubMed:30728504). Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells (PubMed:30728504). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (PubMed:30728504).[UniProtKB/Swiss-Prot Function] |
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