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Grhl2 (NM_026496) Mouse Untagged Clone
CAT＃: MC205946
Grhl2 (untagged) - Mouse grainyhead-like 2 (Drosophila) (Grhl2), (10ug)
CNY 4,664.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0610015A08Rik; BOM; clft3; Tcfcp2l3 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC055035
GGACGAAAGCAGAATTACCTGTAGCTCTTGTTCTGCCATCTCGGGCGCTCTCACACACCTTCACCTGCAC AGACTTGAAAGTCCAGTTTCACCAGAGGCTGAGGCTCCAGGAAAAGGGGAGCGAGTTCATTGGATCAAAC ATGTCACAAGAGTCGGACAATAATAAAAGACTAGTGGCCTTAGTGCCCATGCCCAGTGACCCTCCCTTCA ACACCCGAAGAGCCTACACAAGTGAGGATGAGGCCTGGAAGTCATATCTGGAGAACCCCCTGACTGCGGC CACCAAGGCGATGATGAGCATCAACGGGGACGAGGACAGTGCTGCCGCCCTGGGCCTGCTCTATGACTAC TACAAGGTTCCTCGAGACAAGAGACTTCTGTCTGTGAGCAAAGCAAGTGACAGCCAAGAAGACCAGGATA AAAGAAACTGCCTTGGCACCAGTGAAGCCCAGATCAATTTGAGCGGAGGCGAGAACAGAGTGCAGGTTCT GAAGACTGTCCCGGTGAACCTCTGTCTAAGTCAAGACCACATGGAGAATTCGAAGCGCGAGCAGTACAGT GTATCCATCACCGAGAGCTCTGCCGTCATCCCCGTGTCAGGCATCACCGTGGTGAAAGCCGAGGATTTCA CACCGGTGTTCATGGCGCCCCCGGTGCACTATCCCCGCGCGGACAGTGAGGAGCAGCGCGTGGTTATCTT TGAACAGACTCAGTACGACCTGCCCTCCATAGCCAGCCACAGCTCCTATCTCAAGGACGACCAGCGCAGC ACGCCGGACAGCACCTACAGCGAGAGCTTTAAGGACGGCGCCTCGGAGAAATTTCGGAGTACTTCTGTTG GTGCTGACGAGTATACATATGACCAGACGGGAAGTGGTACATTTCAGTACACCCTGGAAGCCACCAAATC TCTCCGTCAGAAGCAGGGGGAGGGCCCCATGACCTACCTCAACAAAGGACAATTCTATGCCATAACACTC AGTGAGACTGGAGACAACAAATGCTTCCGACACCCCATCAGCAAAGTCAGGAGTGTGGTGATGGTGGTCT TTAGTGAAGACAAAAACCGAGATGAGCAGCTGAAATACTGGAAGTACTGGCACTCCCGGCAGCACACTGC CAAGCAGAGGGTCCTTGACATTGCTGATTACAAGGAGAGCTTCAACACCATCGGGAACATTGAAGAGATC GCATACAATGCTGTTTCCTTCACCTGGGATGTGAACGAGGAGGCAAAGATTTTTATCACCGTGAATTGCC TGAGTACAGATTTCTCCTCCCAAAAGGGTGTAAAAGGACTTCCCCTGATGATTCAGATCGACACGTACAG CTACAACAACCGCAGCAATAAACCCATCCACAGAGCATACTGCCAGATCAAGGTCTTCTGTGACAAGGGA GCAGAAAGAAAAATCCGGGATGAAGAGAGAAAGCAGAACAGGAAGAAAGGGAAGGGCCAGGCCTCTCAAG CCCAGTGCAACAACTCCTCTGATGGGAAGATGGCCGCCATACCGTTACAGAAGAAGAGTGACATCACGTA CTTCAAAACCATGCCCGACCTGCACTCACAGCCTGTGCTCTTCATACCAGATGTTCACTTTGCAAACCTA CAGAGGACCGGACAGGTTTATTACAACACAGACGATGAGCGAGAAGGCAGCAGCGTCCTTGTTAAGCGGA TGTTCAGGCCCATGGAAGAGGAGTTTGGTCCAACACCGTCTAAGCAGATCAAAGAAGAAAACGTAAAACG AGTGCTTTTATATGTGAGGAAGGAGAACGATGACGTCTTCGATGCTCTGATGCTGAAATCACCCACGGTG AAGGGTCTGATGGAAGCGCTGTCTGAGAAGTATGGGCTGCCAGTGGAGAAAATCACAAAGCTTTATAAGA AGAGCAAAAAGGGCATCCTGGTCAACATGGATGACAACATCATTGAGCACTATTCAAATGAGGACACCTT CATCCTCAACATGGAGAGCATGGTGGAAGGCTTCAAGATCACGCTGATGGAGATCTGAGCCCTGGGTGTC CCCTCGATAGGAGCTTTTGGTATACTCCTTCCTGGGAGAGATGGGATCTCTGCCGCCCCAGGACCTGGAG ACCCACCCATCTCACTCACCTCTCAAGACTGTTACAAGACTGCTGGGAAGGGGGCAGGGCCCAAGGCCCA GTAATGGACTTCCTTCAACTCTTCCACTTGCTCCCTATGGAGCTGAAGCCTGAGCCCCTCAGCAAATTTC TTTGAGCCTGGTAGGCCCTTATTTATTGCCCATTTCCCTCCAAGAGCCCTCCAGGACTCTGCAAGTTACT GATAGCTCCATGTGAGACTTCCAGAGTTCCCCCTTCAAGGGAGATAGCAAGTCCACTCATCTCGAACCTG GGGAACTGGTGTGTTTTTTTTTTTCCTTACCTCTCCACATTTCTTGAGTGGTTGGGCCAGATGAGCTGCT TCTTGGTACAGTCTTCATGGATGGGGTAAGAAGGACACCAGTTTGCTGGCCCCAAACCTTCGGATTGCTC TGAGGAAGGTCATTTTGCTAAATACCTCCAGGAGACTCTCAGCAAATAGCCACTGGGCCTTGTACAGGAA GATATCGTGCTACCATGTCAGTAATAAGGAACCCGAGTATGCCTGATGACCATGTACATAACTTATACAA AATATTGCAATAATATATTTTACCTATGGTTGGTGGGCATGTTTACTGCCATTGGCCTGGAGGAGACATA CCTGGAGAATCAGAATGTTCTACTCTATCCAGAGGTGGTTAGGAGAGTGGTCTTTGGGATGTCATATGAT GGTGGTGAATGCCAGGATGGACTCTCCATTGGGCCATGCCTGAGGACTTGTTTCCCCAGAGCCCTCTTTC CTCAAGCATGACTCTCCCTGGAGGCCATTGTGGGTTTTTTGGTGATATATTGTCTCAGCACGCCCTTTGA GCTGTCTGGGAAGGGCCGTACTGTTGGTGCCTTCTCTAGCTTTGGTGTGGGGCATCTCTTGAGCCTTGTG CAAAGTCTCTGCAGAGGTTTGCCTCGGGGCCCTGTCACTGAGACCTGCTGCAGAGCTGCTTGGCCGACGT GGGGATCCTGGACACTGTAGTGCCTCATAGTTTACAAGCACGTCCTTCATCTCAAGTGGCCCCTTTAAAA ACCCACCTGCAGCTTTGTGAACTGAGCCAGCGGCCTTGCTTCTGATGGGAAGAAGTGAGGATGTCCACCC TGGCTGTGGGAAGCGTGGAAGCCCTTGCACTTCTGAAACCAACTCTGGGAGGTGTGCTGGAGAGCTAAGG CTGTCAAGAGAAAATGGCTTACCTGAACAGTCACGCAAGCATGCCCCATGGGCCTCTGTTCTCTTTGAGA GAACAGGTCAATAGTTCTGTTTTAAGGATTGGTATGTGCCCCTGTGGGCCGAGCACTAGTTTGGATAGCC AGGCACATCCCATCCGAAAGCCTGGATAATGGAACAGCTTCTATGGAAAGGTCTAAGTTTGTCTGCGAAG CACTTTGATGTTTTACCTGGAGGCTCTGCTCTCCGGCCCCTGTCTTCTTGCTCTACCTGTCCCGAGGCCC AGAATAGCTGAGGCTGACTCCAGCCAAGCTCTTGCTCTTCAAAGCTGCAGAGAAATTGAGTGGGTTGGAG AGTAGGTGCCGTAGCCAAGAGGATCCCAAAGGCTAGCAGGGGCACCTTTCTGAGAGCCCACTGGACTCCT TATCCCTCTTTGTGTTGCCAAAGGTTTGCATCCTGAGCTATGCTCTATTCCAGCCCCGCACTCCAAATGC CGCTCCTTGAAACTAGCAGTCTAGGGACACTCCAGGGTACGAGTGGGCCTCTGGTCTAAGATGGACAAAC TCTGCTGGAAAGATATGCTCTATGCGCTCCCTGTGCTATAGCACTTGGGGCTAGCTGCAAACGTGATTGG CAGACCTTAGAGGTGGGCTCAGGATTCCAGAGTAGGGAGACAGATCTGTAGCAACCTGACAGGCTACCAG CTCTGGAAAATTGCTGGTCTGCTCTAGGGCAGAAGACAAACCAGAGCCAGCGTTCCCTTTGTCCTGAGTC CCTACAATCCATGAGATAGCCATTGCCAGAACAAGGGATAGGAAGCCACAAAGCATCAGGACAGTGTCAG GTCCAGGATGTCTGCTGGTGGACTTCCATGTCGCTGTGCTGTCTGAGCTCTGCTGTGCCTCGTTTCCTAA ACAAAAACACTCCAGACACATAGAAAATGCTTGCCTTGCCCTGAACTAATCTCACCCAAACAAAGAATTT TTTTTTTTTTTTTGGTAATCTATAAAAGATGAGGGACACATGAAGCAAACATTTCATTCTATGTCCCCAA TTGTCAAAACTTCCTCAGTGTTTAGAAATTGAACCCAATAGAAAAATACTGAGTGAGCTGTCATCTTGAG GTTGGTTCCTAAAAGACTAAGGCACATCCCAATGTAGAAACCTGGGAAGTTTGAAATGAATTAAAACATT TTTCCCCTCATATTTTAATAGTCATAAAAATGCAACAGGAAATAAGGGTTTGTGCCAAATTCTAAGAATG GCCCTTTCTGAAGGACAAGCAGGGGAGACTCGTGTGTACAATCTGTTCCCACATTAAAAAAAAAAGAGTA AATGTAACTTAATAGTTTTGTAAATGGGAGAGGGGGAATCTATAAACTATAAATATGTTATTTTATTTTT TGTACATTTTTAAGAAGAAAAAATAAATATTCACAACATGAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_026496 |
| Insert Size | 1878 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055035, AAH55035 |
| RefSeq Size | 4751 bp |
| RefSeq ORF | 1878 bp |
| Locus ID | 252973 |
| UniProt ID | Q8K5C0 |
| Gene Summary | Transcription factor playing an important role in primary neurulation and in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed:22696678). During embryogenesis, plays unique and cooperative roles with GRHL3 in establishing distinct zones of primary neurulation. Essential for closure 3 (rostral end of the forebrain), functions cooperatively with GRHL3 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (PubMed:20654612). Regulates epithelial morphogenesis acting as a target gene-associated transcriptional activator of apical junctional complex components. Up-regulates of CLDN3 and CLDN4, as well as of RAB25, which increases the CLDN4 protein and its localization at tight junctions (PubMed:22696678). Comprises an essential component of the transcriptional machinery that establishes appropriate expression levels of CLDN4 and CDH1 in different types of epithelia (PubMed:20978075). Exhibits functional redundancy with GRHL3 in epidermal morphogenetic events such as eyelid fusion and epidermal wound repair (PubMed:21081122). In lung, forms a regulatory loop with NKX2-1 that coordinates lung epithelial cell morphogenesis and differentiation (PubMed:22955271). In keratinocytes, plays a role in telomerase activation during cellular proliferation, regulates TERT expression by binding to TERT promoter region and inhibiting DNA methylation at the 5'-CpG island, possibly by interfering with DNMT1 enzyme activity. In addition, impairs keratinocyte differentiation and epidermal function by inhibiting the expression of genes clustered at the epidermal differentiation complex (EDC) as well as GRHL1 and GRHL3 through epigenetic mechanisms (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209534 | Grhl2 (tGFP-tagged) - Mouse grainyhead-like 2 (Drosophila) (Grhl2) |
CNY 5,040.00 |
|
| MR209534 | Grhl2 (Myc-DDK-tagged) - Mouse grainyhead-like 2 (Drosophila) (Grhl2) |
CNY 4,656.00 |
|
| MR209534L3 | Lenti ORF clone of Grhl2 (Myc-DDK-tagged) - Mouse grainyhead-like 2 (Drosophila) (Grhl2) |
CNY 6,460.00 |
|
| MR209534L4 | Lenti ORF clone of Grhl2 (mGFP-tagged) - Mouse grainyhead-like 2 (Drosophila) (Grhl2) |
CNY 6,460.00 |
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