Snai2 (BC062164) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Slug, Snail2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC062164 CAGGGAGCCGGGTGACTTCAGAGGCGCCTGCCTGTCCCCCGCCGCACCTGAGCCACCGCGATGCTATAGG ACCGCCGCCTGGACCGTTATCCGCCCGCGCCCGCCCGCAGCCACCATGCCGCGCTCCTTCCTGGTCAAGA AACATTTCAACGCCTCCAAGAAGCCCAACTACAGCGAACTGGACACACACACAGTTATTATTTCCCCATA TCTCTATGAAAGTTACCCTATACCTGTCATACCAAAACCAGAGATCCTCACCTCGGGAGCATACAGCCCT ATTACTGTATGGACATCGTCGGCAGCTCCACTCCACTCTCCTTTACCCAGTGGCCTTTCTCCTCTTACTG GATACTCCTCATCCTTGGGGCGTGTAAGTCCCCCGCCTTCCTCTGACACTTCATCCAAGGATCACAGTGG TTCAGAAAGTCCCATTAGTGACGAAGAGGAGAGACTGCAGCCCAAGCTTTCAGACCCCCATGCCATCGAA GCTGAGAAGTTTCAGTGCAATTTATGCAATAAGACCTATTCTACGTTCTCTGGGCTGGCCAAACACAAGC AGCTGCACTGTGATGCCCAGTCTAGGAAATCGTTCAGCTGCAAGTACTGTGACAAGGAATATGTGAGCCT GGGTGCCCTGAAGATGCACATTCGAACCCACACATTGCCTTGTGTCTGCAAGATCTGTGGCAAGGCTTTC TCCAGACCCTGGCTGCTTCAAGGACACATTAGAACTCACACTGGGGAAAAGCCTTTCTCTTGCCCTCACT GCAATAGGGCTTTTGCAGACAGATCAAACCTGAGGGCACATCTGCAGACCCACTCTGATGTAAAGAAATA CCAGTGCAAAAACTGCTCCAAAACCTTCTCCAGAATGTCGCTTCTGCATAAACATGAGGAGTCTGGCTGC TGTGTGGCACACTGAGTGGCGCAACCAGTGTTTACTCAAACAGAATGCATTTCTTCACTCCAATGACAAA TGACAAATGAAAGTCCAAAGACATTTTCTCATGTGCTTACCAACCAAATAGTATGTATAAAACCACAAAA GAGTCACACACACACACACACACACACACACACACACACACACAGAGAGAGAGAGAGAGAGAGAGAGAGA GACAGACAGACAGACAGACAGATACACACACACTACAGAACAGAATCTATGTACTTAAAGTTAATTCGTT CTATGTGAAGTTTAAAATTATATTTACTGACAGCTAGATTGAAAGGATAAAAGATAAGAATCTTTCTCTT TAAAGATGAAGTGAAAAGCACATTGCATCTTTTCTTACTAAGAAAGAATACAGAGATTTACACTGCTGCC AAACCATTTCAACCAAAGGAACAGTATTTCTTCTTAATAGAATTGTAATAGTGTTTCCAAGAGGAAGAGA GTCTGCCAGACACTATCTCAGGTGCCTTATAAAGTACTCCAAGTTTACTTCCTTAAATGTATGATGCCTG GTTGTCATCAGTGAATGACAGCCTTTTCTGGATTACCTACAATGTTTTAAAACTATATTGTTAAGAGAAA AAAAACCAAAAACAAGAAAAAGAACAGAACACAAGAGAATGTATTAAAGTATTCTTGTTTTATTTTTGCC ATGTGTGCCTTGGAAGAGGAGGGAAAGACAAACTTCAAACATTCCTGGTGCGTGTCCCATGTCTTTCTTT TTAAAAAAGAATCTTAATGTTTTATAATACAAAGTAATGAAAATGTGCAAAAGAATTTCTTAGACATTCA GTAATGTACTTAGACTTTTGAAAATTCATGTGATGGATGCAGTAATACAATGCCCCTCCAAGTGCCTGTC TTAATGACTTGTGTAGTTGATGAACTGATGTAAATTTGTGTTTATTTTTATACAACTGAATGAACTCTGT ATGAAAGTGAGGTACGGTTAATAGCCACGCCTATATTCAACCAGAATACTTGTGAAATCAATGTCCTTTT TTAAAAAGTAACTTTCAAGGTCTCTTTTTTACAATAAACATTTTTGAGTAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC062164
Insert Size	810 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062164, AAH62164
RefSeq Size	2084 bp
RefSeq ORF	810 bp
Locus ID	20583
Gene Summary	Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis (By similarity).[UniProtKB/Swiss-Prot Function]

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