Akt3 (BC066861) Mouse Untagged Clone

CAT#: MC205969

Akt3 (untagged) - Mouse thymoma viral proto-oncogene 3 (cDNA clone MGC:76721 IMAGE:30089997), (10ug)



CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
AKT3 Antibody - C-terminal
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI851531; D930002M15Rik; Nmf350
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC066861
GCAAAGCACCATTTCTCCAAGTTGGGGGGCTCAGAGGGGAGCCATCATGAGCGATGTTACCATTGTGAAA GAAGGTTGGGTTCAGAAGAGGGGAGAATATATAAAAAACTGGAGGCCAAGATACTTCCTTTTGAAGACAG ATGGCTCATTCATAGGCTATAAGGAGAAACCTCAAGATGTGGACTTACCTTATCCCCTCAACAACTTCTC AGTGGCAAAATGTCAGTTAATGAAAACAGAACGACCAAAGCCAAATACATTTATTATCAGATGTCTTCAG TGGACCACTGTTATAGAGAGAACATTTCATGTAGATACACCAGAGGAAAGAGAAGAGTGGACGGAAGCTA TCCAAGCCGTAGCCGACCGATTGCAGAGGCAAGAGGAGGAGAGGATGAATTGTAGCCCAGCCTCACAGAT TGATAATATAGGAGAAGAAGAGATGGATGCGTCTACAACCCATCATAAAAGAAAGACGATGAATGATTTT GACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAGTTATTTTGGTTCGAGAGAAGGCAAGTGGAA AATACTATGCTATGAAGATTCTGAAGAAAGAAGTCATTATTGCAAAGGATGAAGTGGCACACACTCTTAC TGAAAGCAGAGTACTAAAGAACACCAGACATCCATTTTTAACATCCTTGAAATATTCCTTCCAGACAAAA GACCGTTTGTGTTTTGTGATGGAATATGTTAATGGCGGAGAGCTGTTTTTCCATTTGTCGAGAGAGCGAG TGTTCTCTGAGGACCGCACACGTTTCTATGGTGCAGAAATTGTCTCTGCTTTGGACTATCTACATTCTGG AAAGATTGTGTACCGTGATCTCAAGTTGGAGAATTTGATGCTAGATAAGGATGGCCATATAAAAATTACG GATTTTGGGCTTTGCAAAGAAGGGATCACAGATGCAGCTACCATGAAGACATTCTGTGGCACACCAGAGT ACCTGGCACCAGAGGTATTAGAAGATAATGACTATGGCCGAGCCGTGGACTGGTGGGGCTTAGGTGTTGT CATGTATGAAATGATGTGTGGAAGGTTGCCTTTCTACAACCAGGATCATGAGAAACTCTTTGAATTAATA CTAATGGAAGACATTAAATTCCCCCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCTTGA TAAAGGATCCAAATAAACGCCTTGGTGGAGGGCCAGATGATGCAAAAGAAATCATGAGGCATAGTTTTTT TTCTGGAGTAAACTGGCAAGATGTATATGACAAAAAGCTTGTACCTCCTTTTAAGCCTCAAGTAACATCT GAAACAGACACCCGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAGT ATGACGACGACGGCATGGACGGCATGGACAGCGAGCGGCGGCCACACTTCCCTCAGTTCTCCTACTCTGC AAGCGGACGGGAATAAGTTCCTTTCAGTCTGTTTCTACACTGTCATCTTAGACTTTGCCTGAGACTGATT CCTGGACATCTCTACCAGTCCTCGCTCTTACAGTTAGCAGGGGCACCTTCTGACATCCCTGACCAGCCAA GGGTCTTCACCCTCACCACCTTTCACTCACATGAAACCATATACACAGACACTCCAGTTTTGTTTTTGCA TGAAATTGTATCTCAGTCTAAGGTCTCATGCTGTTGCTGCTACTGTCTTACTATTATAGCAACCTTCAGA AGTAATTTCACAATCTTTGGGAGTCATGAGCCCATTGTTCATTTGTGCATCAAGTGTCATCTTTTGGTTT TTGGTTTTCCCTAGCAGTGAAGGCTAAATGAGATACACTGATTCTAGGTACATTGTTAACTTTCTAGGAG ATAAATCAAGAACTAATTAGACTAAGAAGATTTAGTTTATATTTCTGAACAAGCAATTGTTGAAGGGTGG TGGTGTGTGTATGTAAGAGTTTAGCAGTGTATGTCTGACCAGCACATGAAGTGTGTATCACTGACATTTG GCTGGAAAATCTTAGGAAACATTGAGAGGACTGAAGGGCCAATGAGATACACATTTTTTTTTTTTTAAAT CCTGCTGGCTATTGTGGAGGAAGCCCAGTGCCACCCATGGATGCCCAGGTTTCAAGGGCAAATGGACATT GTTCAACCAAGTACTGACGGTGCAGAGTCCCCTAGAGAGTCTTAGGATCTACTTTGTACCAGAGAGTATT TTCCCCTGGGCTTGGCAACCTTTTTCCCTTCTTTATATTTATCACCTCTGATGGCTGAAGAATGTAGACA GTATAATGATCCTGCTGTCGCCAAAATCTATACACCAAGGTGAACAGGTGTTTGCCTTACCAGTTTTGGA GTTTTTACTTTCAGTTATAGGTAAATTAGCTTTTTCTCAGATGTTAAAACCTTGAATGTCCTTTATGGTT TTGTTTATATTACAGTAGTATTTATTTTTTAGTGGTAAGAATTGTGTTGTCATGTTAGCAAGCGCAGCTC CAATTCACAGATCATTGCCTGCGTTTTCTTTTGACCCATGTGCAAGGAATGTACACACCCATTAGAATCA TGCACTTTTTCTCCTATGTTAAAAACAATGGTAAGGCTCTGGGACTGTATCTGTTGGTTAGAATCTGGCT AGAATCTCTGGGAGAAGAGATGCTGTGATTTAACCCCTTGGAGCTGAAATGAGAGCATCCCCAACTACCC AGTGCCAAGGGATGGATGTGAGAGGATAGTCTGGCAGAAAGTAATGGAGAAAGCTGAGACCCAAATGATG ATGAGAAATGAACTCTACTCGGCAAAATGAACACATCACAAGTATTCACACAGTAGGAAGTAAAGGATAA GAAGCTGATGTGTTTATTCTTATCAAGGTACATTCATTACTGAATCAAGCCATCAGGGAAAACAACAAAA CAAAACACCTCTAGCTTTTATTTTTCTGTACATATTCATGTCTCCCAACCCAAACATTTCTCACTGAGAG GGGACATGTATGCAAACCTCATCTTTCTCCTTCATTAATGATGATCTTCAGATTAACCCTTTGGTGCTAG GAGCTGACACTTTCCAAAGCAGACTATGACGTCCAAGGATCAAAGCCTCTCTTGCAGCAAGCAAGAGCAA CTGCTGCTCCAAGGCGTGATCATGGAAGTGGAAGACCACAGTGTGACACAGTGGTACTGCATTGTCTTTT TCAGTAGGAAGTCATGTTCTCATGCAAGTGTCTTGGCCTCTAGGCTCAGACTTAGAGAAGCAGAGCCGCT GCTGGCAGTGGCTGGCCGCTGGGACTGCTGGGGTGTAAAGGGCATTTTCCTAAGGCAGCTAAGACAGACT CAGACATAGATTTTTGTCCTAATTTTTCATGTTTCTACCTGGGTGAGTTTACCCTCAGTGGGAAGTAGGA AGTTAAACAGGTGTATTCTTACATCTGCTGTCTTCTGTTTTCCTACATAGAAGTATGTTGAATGTTGCAC ATTGACAAGGAAGTAGATTAGATGGTAAGTCCTTTTAAGTTACCCTGCATCCATTCTGTAAGAAAGGAGG AGGAGAGGAAGAAAAGCAAGCTGAGCCTGGCCGATGTTCAGAACATTTACTCCCTCAGAGGGAGCAGACA CTGTACACTAAGCACACAGCTTCTTTTGTTTTGTCTCTCCTTCCAAGTCTTAAGGTAATTTCATGACCTT GGCCAAGGATACTTTGTGAAGATTAATGAGGGGACATTGACAATGCCTCAGGCTGGCCACTCCTCACACT GGCCCTTGGTCCCCAGCCATTCAAGGGGTTACTCATCCCATCCCTGACTACCTGAGTGTCACTACAGGTG GGTTCTTGGCTTTGAGTTCAAGAATTCTTTCCTTACACCCTCCACCAGATGTCAGTGGAAAGGCAGAAGT AGAACCGAGGTGTGGGAGGAGATTCTAAAAGCTTGTGTTGCCCAGGCCTCTAGCTTAGGGTTCTGGCAAT TGAGACGGCCTGAAACCTGACAGCATCATGTCCGGGCAGCTCTGGCTTTGTGAGTCCAGCTCCTACCAAC TCCACCTTGACCTCTTGTCTCCATGCAGGGCTGTCTGGGAGGAAACTGGCCACTTCTGCTCAGACTGCTG CCAGCGCTCTCACAGCTTTGCTCTCTACACTAATGACATAGATTATTCCAGTATTGTTCCCATTTCCCAC CTGACCTCCAGCTTCTCGGAGCTGACTTCTTGCAGGGGCCACATGCTTCTTTCCCTCACTAACTGCAGGG TCTCCACCACACCTCAGTGTACACACTTTGCTGCTACCGTCTGTACTGTCTACATCACGGTTCCCTTAGC TTGCTCCTGGTAGTGCATTACAGGCAAGCATGAAATGTAAAGTATTTATTTAAATAAAAAGGAAACCTCT GAATTGGTCATCGAGTCACCTCCCTGTAGATTTGTAGTCTGTGACATATTTCGACTTTCTAGTCCTGCTA GATCCATATAAGATCTGGTCATCTGGTTAAGGGTTTGAAGCAGACATAGCTATGAACCCGGATGGTATCA TCACTGTCACGGCTGGCCACACTACAGATGTTTATGTCCTTACGTTTATGACCCACAGGGTGTGAAGTAA CTTCCAAGAACCTGATTTTGTACACTGTCCACTCTCAGCATCTCACACTCTCTGATAGGGACACACATTA CTTCCTTCGTACAGACAGCTTAAATAAAGCCCTATGTCAATCTGCAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN BC066861
Insert Size 1440 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC066861, AAH66861
RefSeq Size 4751 bp
RefSeq ORF 1440 bp
Locus ID 23797
Gene Summary AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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