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Ddx11 (BC079656) Mouse Untagged Clone
CAT＃: MC206095
Ddx11 (untagged) - Mouse DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) (cDNA clone, (10ug)
CNY 6,456.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CHL1, KRG2, CHLR1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC079656
GGGGCGTGGCCACGTGGAGGGTTTTCTGCGTGCGCGCATGCTCCTTCAGTGAAACTGCAGTTTAAATTCA GCCGCGGCGGCCAGGCTCGCTTGCCGGCCTCAGTGAGGCGGTCCTGCGGTGGCGATCGAGTTCGGTGCTG TGTGGGAGAAGCGCTGCGACCGAAGGCAGAGAGGGCAATGGCTGACGAAAACCAGGAGATTGGTGGCATC CATTTTCCTTTTCCCTTCCCGCCCTATCCTATCCAGAAAGACTTCATGGCAGAGCTCTACAAGGTTTTGG AAGGTGGCAAGATTGGGATATTTGAAAGTCCAACTGGCACGGGAAAGTCCTTAAGTCTGATTTGTGGGGC CCTTTCCTGGCTGCGTGACTTTGAAAAGAAGAAATTGCAAGCAGAGGCTCTTCTCCTTGCACCTGGATCC GGCCCTCCAAGTAGTGAGAAGAACTCGCTCCTGACTTCCTCATCTTGCCAAGAACCTACGGACACCCCGA GGCCTGCTGGAGAGCCTGACTGGGTCACCGAATTTGTGCAGAAGAAAGAAGAAAGGGACCTGGTGGAGAG GCTAAGGGAGGAGCAGAAACATGAGGAAGAGGAGACTGAGGCTCTCCTGCGCCTCAGCAGGGAGATGCTG GATGCAGGGACAGGACCAGAGCAGCTGGAGCAGCTGGAGTGTGGGGAGGAGCACCTGGTTCTGGCAGAAT ATGAGAGTGATGAGGAGAGAAGAGGCAGCAGGGTGGACGAGGCTGAGGATGACTTGGAGGAAGAACATAT AACCAAGATTTATTACTGCAGTCGGACACACTCACAGCTGGCCCAGTTTGTGCGGGAAGTACTGAAGAGC CCTTTTGGCAAGGAGACCCGGCTAGTCTCACTTGGCTCTAGGCAGACCCTTTGTGTGAATGAAGATGTGA AAAACCTGGGTTCTGTGCAACTTATGAATGACCGCTGTGTGGACATGCAAAGAAGCAAACGCGAAAAGAA CGGGACTGGGGAAGACAAGCCAAAGAGAAAGAGACAGAAGATCCAGACCTCTTGCCCCTTCTACAACCAC GAGCAGATGGAGCTTCTCCGGGATGAGATTCTGCTGGAAGTGAAGGACATGGAACAACTTGTGGCCCTTG GGAAGGAGGCCCGGGCCTGCCCCTATTACGGAAGCCGCTTTGCCATCCCTGCAGCCCAGCTAGTGGTGCT ACCCTACCCAATGTTGCTGCATGCAGCTACCCGACAGGCTGCGGGAATCCGGCTACAAGGTCAGGTGGTC ATTATTGACGAGGCTCACAACCTGATCGACACCATCACCAACATCCACAGCACAGAGGTCAATGGTTCCC AGCTCTGCCAGGCCCATTCCCAGTTGCTCCAGTACATGGAAAGATACAGGAAGCGTTTAAAGGCCAAGAA CTTGATGTACATCAAGCAGATCCTGTACTTGCTGGAGAAGTTTGTGGCTGTTTTGGGAGGTAATGTTAAG CAAAATCCTACTACACAGAGCCTGTCTCAAACAGGGTCTGAGCTGAAGAGCATCAACGACTTTCTCTTTC AGAGCCAGGTGGACAACATCAACCTCTTCAAGGTGCAGCGGTACTTGGAGAAGAGCATGCTCAGCAGGAA GCTCTTTGGCTTCACAGAATGTTTCGGAGTGGTTCTTCCATCCCTCTCAGACTCTCAGGAAAACCGAGGT CTGGCTGGCTTCCAGCAGTTCCTGAAGAGCCTGCAGTCAGGGCCTACTGAGGACTCCCCGGAGGAGGGCC AGGCTGTAGCCCTGCGGCCTGCCTCTCCACTGATGCACATCGAGGCCTTCCTGGCAGCTCTCACCACAGC CAATCAGGATGGCAGGGTTATTGTGAACCGCCAAGGCAGTGTGGGTCAGAGCAGCCTTAAATTCTTACTC TTGAACCCAGCTGTGCACTTTGCCCAGGTGGTGAAGGAATGCCGAGCAGTGGTCATTGCAGGTGGCACCA TGCAGCCGATGTCTGACTTTCGGGAGCAGCTGCTGGCATGTTCTGGAGTAGAAGCGGGGCGAGTGGTGGA GTTTTCCTGTGGTCACGTGATCCCTCCAGACAACATCTTGCCCCTTATTATATGCAGTGGGCCCTCTAAC CAGCAGCTGGAATTCACCTACCAGAGAAGAGAGCTGCCTCAGATGGTGGAGGAGACAGGCCGCATTCTCT GTAACTTGTGCAATGTGGTCCCTGGAGGTGTGGTATGCTTTCTCCCTTCTTATGAGTACCTGCGCCAGGT ACATGCTCACTGGGACAAGACTGGCCTGCTGACACGTTTGTCTGTCAGGAAAAAGATATTTCAAGAGCCC AAAAGAGCGAGCCAGGTGGAGCAGGTGCTGATGGCGTATTCCAAGTGCATTATGAGCTGCAGTCACTCAG AAGGCCATCTGACAGGGGCCTTGCTCCTCTCTGTGGTTGGAGGGAAGATGAGTGAAGGGATCAATTTCTC TGATGACCTGGGCCGGTGTGTGGTGATGGTGGGGATGCCATATCCCAACATTAAGTCTCCAGAGCTGCAA GAGAAGATGGCCTACTTGAATCAGACACTTCCTAGGACACAAGGTCAGCCCCTGCCAGGGACGGTATTGA TAGAGAATCTGTGTATGAAGGCTATAAACCAATCCATAGGCAGGGCCATTAGGCACCAGAGGGACTTTGC CAGCATTGTGCTCCTGGATCACCGGTATGCGCGTCCTTCTATTCTGGCGAAGCTGCCAGCCTGGATACGA GACCGAGTGGAGGTCAAAGCTACCTTTGGGCCTGCATTTGCTGCTGTGCGGAAGTTTCATCGAGAGAAGT CACACCCAAGTCTGGTATAAGCGTCTCATACCCTGCTTGACCTGGCCTTTGTATTTGGCCTAAGAAGATA GAAACCATATGCAGACTGCCAAGTGTACCCCAGCCTTCCAGAGGCAGTGGACTCTAGTGTTCAACTTGCA TAGAAAAGCCAGCAGAACATCCTGAAGGAACCTGGCCCGAGACACCTTATTCCCTGCTCAGGTTCAACTT TCCGCCTGGCTTCAGGAGGCTGTGCTAGCAGCAGCTTTCTGCCTGTTCTGAGGTGAGACCCACCTGCCTC TGCCCTTTCTGGAGACAGTTAGCTTTTCTTGGCCTTTGACTCCCATCTCAGGCACCGATGTCCCCGTTTC AAGCATCATTGCCTGACCCACTGCAGTATGACACACACACCATCTGTGCAGAAGAGAGTTTTGAGAAAAA ACTAGGACATACATAATATTGAAAGAGCTGACATGAATTTTGACTTTTCTGAAGGACCATCACAAAGACT TGGACCCTGGCCATAGACCTTTCTGTGTCCCACAATTTTTTCTCCTTCTACTTTTCCTCATAAACTAAGA GGCATTGATCCTGTCCATGGCTGTTCAGGACTCTGTGCTCAGAACATCTACCTGAAGACATGTCATTGGG CAAAAGTGTCTCCGTGTTTTTCATTTTTTTCTGCTGTCCCATGAAGGAGAACCCCAGTTAAGAGTCCAGC TCCTTTATATTCAACTGTGGCTGTCCCTTGTGTGAGCTTCAGCAACCTCAGGGGCAGTGCCCTTGCCATT TAGGCAATACACTGGTTGCATACTGCTTGCAAACTGAGAGGAGCCATAGAAGAGGACTTCTTTTTCACAG TTCTTGTGCCGCTTACAGCTCAAAAGAATTCCATTCTTGAATCTTCCTCAGATTGAAATGGTAAGGCTTC TCCAAGCAGTGGTGGCCCACGCCTTTAATCCCAGCACTTGGGAAGCAGAGGCAGGTGAATTTCTGAGTTC GAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGGCAGCCAAGGCTATACAGAGAAACCCTGTCTTGAAAA CAAACAAACAAACAAACAAATGGTAAGGCCTCATACCTGAGGGATAGTGTTTAGAGACAGGACTCCTGTA GGGAGGTAGGGACAGATTTTTTTGTCACTAGTGTAGGGCTTTAGCATACACTAAAGTGCAGCTAGAAGTC CCCATCATAGGAGGACCCAGAGAATGGCAGCCTCTGTAAACCTTGAAGGGTTGTCACTGGGAATCCACTC ATCTGTTACCTGTTATAGCAGAGTTCCTAGGCTTACATCCTAGGAATGTCATCCATGTGTCATGGCCGAT TAAAAGAACAACTGAACCTAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | BC079656 |
| Insert Size | 2643 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC079656, AAH79656 |
| RefSeq Size | 4167 bp |
| RefSeq ORF | 2643 bp |
| Locus ID | 320209 |
| Gene Summary | DNA-dependent ATPase and ATP-dependent DNA helicase that participates in various functions in genomic stability, including DNA replication, DNA repair and heterochromatin organization as well as in ribosomal RNA synthesis. Its double-stranded DNA helicase activity requires either a minimal 5'-single-stranded tail length of approximately 15 nt (flap substrates) or 10 nt length single-stranded gapped DNA substrates of a partial duplex DNA structure for helicase loading and translocation along DNA in a 5' to 3' direction. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended up to 500 bp by the replication protein A (RPA) or the cohesion CTF18-replication factor C (Ctf18-RFC) complex activities. Shows also ATPase- and helicase activities on substrates that mimic key DNA intermediates of replication, repair and homologous recombination reactions, including forked duplex, anti-parallel G-quadruplex and three-stranded D-loop DNA molecules. Plays a role in DNA double-strand break (DSB) repair at the DNA replication fork during DNA replication recovery from DNA damage. Recruited with TIMELESS factor upon DNA-replication stress response at DNA replication fork to preserve replication fork progression, and hence ensure DNA replication fidelity (By similarity). Cooperates also with TIMELESS factor during DNA replication to regulate proper sister chromatid cohesion and mitotic chromosome segregation (PubMed:17611414). Stimulates 5'-single-stranded DNA flap endonuclease activity of FEN1 in an ATP- and helicase-independent manner; and hence it may contribute in Okazaki fragment processing at DNA replication fork during lagging strand DNA synthesis. Its ability to function at DNA replication fork is modulated by its binding to long non-coding RNA (lncRNA) cohesion regulator non-coding RNA DDX11-AS1/CONCR, which is able to increase both DDX11 ATPase activity and binding to DNA replicating regions (By similarity). Plays also a role in heterochromatin organization (PubMed:21854770). Involved in rRNA transcription activation through binding to active hypomethylated rDNA gene loci by recruiting UBTF and the RNA polymerase Pol I transcriptional machinery (By similarity). Plays a role in embryonic development and prevention of aneuploidy (PubMed:17611414). Involved in melanoma cell proliferation and survival. Associates with chromatin at DNA replication fork regions. Binds to single- and double-stranded DNAs (By similarity).[UniProtKB/Swiss-Prot Function] |
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