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Plod3 (BC043047) Mouse Untagged Clone
CAT＃: MC206114
Plod3 (untagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (cDNA clone MGC:57947 IMAGE:5703967), (10ug)
CNY 5,432.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI414586; LH3 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC043047
CAAACCTAATTCCGGTCGCATTGTGGTTACCCAAAAAAAAAAAAATCTGAGAGCCGCCTGCAAATGGTCA CTTTTTAAAAACTGGCGTGGGTGGTCTTTAGGGGAGCGCTCTTGTCCCTGTGCCCTTTGCATCCCCGGTG CGTGTCCCCGCGCTGCGGGACAGGATAGGCAGGTTCGGTGGCTTTGTTGAACCCAGACCCACGAGGATGT CCGCGCGATCCCGCCTCCGGCTTCCCGGCCCCTCCGGGCCCGTCGCCCCCGCCGCGCGCCACCCCAGGCC TTGCTGTCCTGTGTTCCTCCGGGTCAGACTTGTGGTCAAGACCCGCCCCCGCGTCGCCTGGACCCTCCTC AAAAGGTCACTTCCCCTCCCGGGGGGATTCGGTTGCTCCGGTGTGAGTCAGGCAGTGCCCAGGACCGGAC CCTCGGCTCCTCCCCACCCTCCAGCCCGCGCCCGCGGGACAATCCTTGCCTTGTCTCGCCGTCTGCATCT CGGGTTTTCGGTGGCCTCGGGTTACGCCTTTTTTCCAGGGCGGAGTCCCGAGCACAGCTCCTCCTCTCAG CGGGCTCGGTTTCCCCTTTTCCCAGAATCTTCCCGGGGCGTCGGAATCCAGGCGCTCAGCTCAGCGCCGG TGCGGGCCTGGGTGTAGCCTAAGCGTGCTGCTAGCCATGGCTGCGGCAGGCCCGGAACCCCGGCTTTTGC TCCTGCTCCTGCTGCTGCTGCCGCCGCTGCCCCCCGTAACTTCTGCCTCCGATCGACCCCGGGGCGCCAA TGCTGTCAACCCAGACAAATTGCTGGTGATCACTGTGGCTACGGCAGAGACAGAGGGGTACCGGCGTTTT CTGCAGTCTGCGGAGTTCTTTAACTACACTGTACGGACCCTGGGCCTGGGACAGGAGTGGCGAGGGGGTG ATGTGGCTCGAACAGTTGGTGGAGGCCAGAAGGTCAGGTGGCTCAAGAAGGAAATGGAGAAATATGCAGA TCAGAAAGACATGATCATCATGTTTGTGGACAGCTACGACGTGATTCTGGCAAGCAGCCCGACAGAGCTG CTGAAGAAGTTTGTTCAGAGTGGCAGTCATCTGCTGTTCTCTGCTGAGAGCTTCTGCTGGCCTGAGTGGG GGCTGGCAGAGCAGTACCCTGAGGTGGGCATGGGGAAGCGCTTCCTCAACTCTGGTGGATTCATCGGCTT TGCTCCCACCATCCATCAGATTGTCCGCCAGTGGAACTACAAAGACGACGATGATGATCAATTGTTCTAC ACTCAACTGTACCTGGACCCAGGGCTGCGGGAGAAACTCAAACTGAGTCTGGACCATAAATCCCGGATCT TTCAGAACCTCAATGGAGCCTTAGATGAAGTGATCTTAAAGTTTGACCAGAATCGTGTGCGCATCCGGAA TGTGGCCTACGACACACTTCCTGTTGTGGTCCATGGAAATGGTCCCACTAAGCTCCAGCTCAACTACCTG GGGAACTATGTCCCCAATGGCTGGACTCCCCAGGGAGGCTGTGGATTCTGCAACCAGACCCTGAGGACAC TCCCGGGGGGGCAGCCTCCCCCCCGGGTGCTTCTGGCCGTGTTTGTGGAGCAGCCTACTCCCTTCCTGCC TCGGTTCCTGCAGCGCCTACTGCTCCTGGATTACCCCCCGGACAGGATCTCTCTCTTCCTTCACAACAGC GAGGTGTACCACGAGCCTCATATTGCAGATGCCTGGCCACAGCTCCAGGACCATTTTTCAGCTGTAAAGC TGGTGGGGCCAGAGGAGGCCCTGAGCGCAGGGGAGGCCAGGGACATGGCCATGGACAGCTGTCGGCAGAA CCCTGAGTGTGAGTTCTACTTTAGCCTGGATGCTGATGCCGTCCTTACGAACCCGGAGACCTTGCGTGTC TTGATTGAACAAAACAGGAAGGTAATAGCTCCCATGCTTTCCCGCCATGGCAAGCTCTGGTCCAACTTCT GGGGTGCCCTGAGCCCCAATGAGTACTACGCCCGCTCCGAAGACTACGTGGAACTGGTGCAGCGGAAGCG AGTGGGCGTGTGGAATGTACCCTACATATCTCAGGCATATGTGATCCGCGGGGAGACCCTGAGGACTGAA CTGCCCCAAAAGGAGGTATTCTCCAGCAGCGACACAGACCCAGATATGGCTTTCTGCAAGAGCGTCCGGG ACAAGGGCATCTTCCTCCACCTCAGCAACCAGCACGAGTTTGGCCGGCTGCTGGCTACTTCTCGCTATGA CACAGACCACCTACACCCAGACCTCTGGCAGATCTTTGACAACCCTGTGGACTGGAGAGAACAGTACATT CACGAGAATTACAGCCGGGCCCTGGATGGGGAAGGGCTAGTGGAACAGCCATGCCCAGATGTGTACTGGT TCCCACTGCTGACCGAGCAGATGTGTGATGAGCTGGTGGAGGAAATGGAACACTATGGCCAGTGGTCTGG AGGCCGGCACGAGGATTCCAGGTTGGCTGGAGGATACGAGAATGTTCCCACCGTTGATATCCACATGAAA CAGGTGGGATACGAGGACCAGTGGCTTCAGCTGCTGCGGACATATGTGGGGCCCATGACGGAGTACCTGT TCCCTGGCTACCACACCAAGACACGGGCAGTGATGAACTTCGTGGTCCGCTACCGGCCAGATGAGCAGCC CTCGCTTCGGCCACACCATGACTCGTCCACCTTCACTCTCAATGTCGCCCTCAACCACAAGGGTGTAGAT TATGAGGGAGGCGGCTGCCGCTTCCTGCGTTACGACTGCAGAATCTCCTCTCCGAGGAAAGGCTGGGCCC TCCTGCACCCTGGCCGCCTCACACACTACCATGAGGGGCTGCCCACCACCCGGGGTACTCGATACATCAT GGTGTCCTTTGTTGACCCCTGACACTCAACCAGTCTGCCAAACCTTCTCTGCCACTGTGCCTTGTTGGAC AACCTGGGTCGCCATTCTCAGAGAGAGGGGACGCTGCCCGTCTCATCTCCCGAGAGTGTCTGTGTGCCTG GGATGGAATGTGCTGGTCCCAACAGTCCTCAGGAGGTCCCTGGAGTCTTCCACTTGCCCACTGACCCCAT GGACTCAGGGAAAGTGGACTTCTGAGGGTCCCCTGCCTGATAAATTATTAAGCTTCCTCAGCCTCACTTT CAATAAAAGGATGTTTGTAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | BC043047 |
| Insert Size | 2226 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC043047, AAH43047 |
| RefSeq Size | 3186 bp |
| RefSeq ORF | 2226 bp |
| Locus ID | 26433 |
| Gene Summary | Multifunctional enzyme that catalyzes a series of post-translational modifications on Lys residues in procollagen (PubMed:16447251). Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:16447251). Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine (By similarity). Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:16447251, PubMed:16467571, PubMed:21220425). Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:25419660). Catalyzes hydroxylation and glycosylation of Lys residues in the ADIPOQ collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:23209641). Essential for normal biosynthesis and secretion of type IV collagens (PubMed:15377789, PubMed:16467571, PubMed:17873278). Essential for normal formation of basement membranes (PubMed:15377789, PubMed:16467571).[UniProtKB/Swiss-Prot Function] |
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