Qars (BC079854) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110018N24Rik; 1200016L19Rik; C80286; GLNRS
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC079854 GGTGTGCCTGCTATGGCTACTCCAGATTCGCTAGCGCTGTTCACCGGCCTTGGCCTTAGCGAGAACAAGG CCCGCGAGACGCTCAAGAACGAGGCTCTGAGCACTCAGCTGCGCGAGGCGGCGACCCAGGCACACCAGAT TCTGGGTTCTACCATCGACAAGGCTACTGGGGTCCTCCTATATGACTTGGTCTCCCGACTCAGGGATACT CGGCGTCGTTCTTTCCTTGTGAGCTATATAGCCAATAAGAAGATTCACACGGGACTCCAGCTGAGCGCTG CTCTTGAATATGTTCGGAGTCATCCCCAGGATCCCATTGATACCAAGGACTTCGAGCAGGAGTGTGGCGT CGGTGTGGTGGTGACACCGGAGCAGATTGAGGAAGCTGTGGAGTCCACCATAAATAAGCATCAGTTGCAG CTCCTAGCGGAACGGTACCGTTTCAACATGGGGCTGCTAATGGGAGAGGCTCGGGCTGCGCTCAGATGGG CAGACGGCAAAATGATCAAGAACGAGGTGGATATGCAGGTGCTTCACCTTTTGGGGCCCAAGATGGAAGC TGATCTGGTGAAGAAGCCCAAGGTGGCAAAGGCACGGCTGGAAGAAACAGACCGGAAGACAGCAAAAGAT GTGGTGGAGAAAGGTGAAGTGGCTGGCCAGACCCTGTCTCTGATGGAGCAGCTCCGGGGGGAGGCACTTA AGTTTCATAAGCCAGGTGAGAACTACAAGACGCCAGGCTATGTGATCACTCCATATACCATGGATCTGCT GAAGCAGCACCTGGAGATCACTGGGGGACAGGTACGTACGCGGTTCCCTCCAGAGCCCAATGGAATCCTG CATATTGGACACGCCAAAGCCATCAATTTCAACTTTGGTTATGCCAAGGCCAACAATGGTATCTGTTTTC TGCGCTTTGATGACACCAACCCTGAGAAAGAAGAAGCAAAATTCTTCACTGCTATTTATGACATGGTGAC CTGGCTGGGTTATACACCTTACAAAGTGACATATGCTTCTGACTATTTTGACCAGCTGTATGCCTGGGCC GTGGAACTCATCCATGGGGGTCTTGCTTATGTGTGTCACCAGAGAGTGGAAGAGCTCAAAGGCCATAACC CTTTACCTTCTCCATGGAGGGACAGGCCTAAGGAAGAATCATTGCTGCTCTTTGAGGCAATGCGCAAGGG CAAATTTGCAGAAGGAGAGGCCACACTTCGAATGAAGTTGGTGATGGAAGATGGCAAGATGGACCCCGTG GCCTATCGAGTCAAGTACACGCCACACCATCGCACAGGGGACAAATGGTGCATCTACCCCACCTATGACT ACACACATTGTCTGTGTGACTCCATTGAGCACATTACCCACTCGTTGTGTACCAAGGAGTTCCAGGCTCG ACGGTCTTCCTACTTTTGGTTATGTAACGCTCTGAAAGTCTATTGTCCTGTTCAGTGGGAATATGGTCGC CTCAATTTGCACTATGCTGTTGTCTCAAAGCGCAAGATTCTCCAGCTTGTAGCAGCTGGTGCTGTTCGGG ACTGGGACGATCCACGGCTCTTCACACTCACTGCCCTACGACGACGGGGTTTTCCACCAGAGGCTATCAA CAACTTCTGTGCTCGGGTTGGGGTCACAGTGGCACAGACCACAATGGAACCTCATCTTCTGGAAGCCTGT GTGCGTGATGTGCTGAATGATGCAGCCCCACGTGCCATGGCTGTTCTAGAGCCACTACAAGTTGTCATCA CCAACTTTCCTGCTCCCAAGCCCTTGGACATCCGAGTGCCAAATTTCCCAGCTGATGAGACCAAGGGTTT CCACCAGGTTCCTTTTGCTTCCACTGTCTTCATTGAGAGAAGCGACTTTAAGGAGGAGTCAGAACCAGGC TATAAGCGCCTAGCTTCGGGCCAGCCTGTGGGCCTGAGACACACTGGTTATGTCATTGAACTGCAGAATA TTGTCAGGGGCTCCAGTGGCTGTGTGGAACGCTTGGAGGTGACCTGTAGACGAGCTGATGCTGGAGAGAA GCCCAAGGCCTTTATTCACTGGGTATCACAGCCTTTGGTGTGTGAGATTCGCCTCTATGAGTGCCTATTC CAGCACAAGAACCCCGAAGACCCTGTTGAAGTGCCTGGTGGATTCCTAAGTGACCTGAACCCGGCGTCAC TACAAGTGGTAGAAGGAGCATTAGTGGACTGCTCTGTGGCTTTGGCAAAGCCCTTTGACAAGTTCCAGTT TGAGCGCCTTGGGTACTTCTCTGTGGATCCAGACAGCCATCAAGGACAGATTGTCTTCAACCGAACTGTC ACACTAAAAGAAGACCCAGGCAAAATATGAACTGAATACATGTGGCCCTACCTCATCCTGGAGGCTGGGG GTGCCCTTCAGCACTCCACACTCCGTTTCAATAAAGAATAACTAAATCCCTAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC079854
Insert Size	2328 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC079854, AAH79854
RefSeq Size	2460 bp
RefSeq ORF	2328 bp
Locus ID	97541
Gene Summary	Glutamine--tRNA ligase. Plays a critical role in brain development.[UniProtKB/Swiss-Prot Function]

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