Calm3 (BC050926) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CaMA; R75142
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC050926 TGCGGGCAAAGAGTGTGGAGGCGCGGACGCGCGGCGGAGCTCGAGCTGCTGCAGCTGCTGCCGCCGCCAG AGTAACCTCGACCCCCGAGCTCCGGATACCGGTGCCTCGCCATGGCTGACCAGCTGACTGAGGAACAGAT TGCAGAGTTCAAGGAAGCCTTCTCCCTCTTCGACAAGGATGGAGATGGCACCATTACCACCAAGGAGCTG GGGACTGTGATGAGATCGCTGGGACAGAACCCCACTGAGGCGGAGCTGCAGGACATGATTAATGAGGTGG ATGCTGATGGCAATGGGACCATTGACTTCCCAGAGTTCTTGACCATGATGGCCAGAAAGATGAAGGATAC AGACAGCGAGGAGGAGATACGAGAGGCCTTCCGTGTCTTTGACAAGGATGGGAATGGCTATATTAGCGCT GCCGAGCTGCGTCACGTCATGACGAACCTGGGGGAGAAGCTGACAGATGAGGAAGTGGATGAGATGATCC GAGAGGCCGACATTGATGGAGATGGCCAGGTCAATTATGAAGAGTTTGTACAGATGATGACTGCGAAGTG AAGGCCCGGGCAGCTGGCCATGCCCGTTCTCCTGATCTCTTCTCGCGCTCTCCTCTCTTCAACACTCCCC TGCGTACCCGGTTCTAGCAAACAGCAATTGATTGACTGAGAATCTGATAAAGCACCAAAAGATTTGTCCC AAGCTGCATGACTGCTCTCTCTCCTTCCTCCTGACTGTCCCTCCACGCCCTCACCGCTTCCTTTGGCCTT CCCCTTCCATTCCCAGTCTCCAGGCCTGATGCATTCACAAGGTGAACCCCATTGCCCTGCCCTGGGGAGC CTCTGCCCTCCTCCTCCAGCCCGGATGGCTCTCCTCCGGTTTTGGTTTGTTTCCTTTTGTTTGCCCTTCT TTTGGGTGCCGGGGTGGCCACCATTCCTGTCCCTGCTCAGTGGGGAGGGGAGCAAGGCCTTCTGCCAGGT GGAGGAGCATGTCCCCTGCCACTGTCGCATGCACCCCAGCCCTGTGACTCTGTGTGCAAGCCCAGCAGCC TGTGGGGCGGGGTGCCAAGAGGGGATGACGGGACGTTCCAGGACCGAGGGAGTTGAGGATGTGACCCAGG ACGGGGGGCGGGGGGGGGGGTTGTGTGTGTGGAAGGGTGGGAAGAGGCAGGAAGGCCTTGGAAGCTAACA TGCTTTCGCTACTGGAGGGGGACTGCATAGAGTGGACGGCACTCGGGTCTCGCTGCCGCTGTTCTGAAAC CATCTGGCTGGCTTTCTGAGGTCAGGCTGGGTGGGGGGCTGTCATCGGCCATGCTGCCGATCATACTCGC CCTCCTCATCCCTCCAGCCATGTCCCGCTGTTCTGTAAATACCTGGTGCTAACATCCCATGCCGCTCCCC CGTGGCACCCCCAGCCACCTGCCCAGTTCAGGGCTGGTCCAGGAATGGATGTGGGGGATGGTCGCTGTGT AATGTGCCGGTGATCTTTTTTTCCCCTTCCCCTTTATGCCCCTTAAAACTTTGATTTTTGTCCTAAACAT GCCGGGCCAACTAAAGGGTGGGGAGAGGAGAGATGCGCCCCACCATGCTCACAAGAACCAACCTGCAATA AACACTTCTATGGGCCGCCGCGCAGAGCACGCAGCGGGCACCTCCTGCCCCTCCGTGGTGACTTGGCATC GCTTTCTGCCCTCGCCCTGCCTGCCCCACTCCCCAGCGGAGAGCATGATCCGCACCCTTGCTTCTGACTC TAGCCTCTGGGACACGTCAGTCAACGCGGGCAGTTCGGTCTGGGTTCCTTTCCTTTCTCTGTTCTCATCT GGCCCCCCAGGGTGATCTTGTTGCTTTTTGCTGGGACCTGCCAGCTTTGAGACTCTCCACCCCTTGGCAC CAGCCTTAAGGGAGGGAGGGACAGAGCAAATCAGGAGACCCAGCCTAGAGTTGAGGGCAAGGGCAGGTAG GCGTGAGGCTGTGGACTTTGCGGAATGTTTCGGGGTTTTATTTTGGTTTTTTGTTTTTCCTTTTTTTTTT TTTTTTTTAAACAAACAAACAAACCGGGCAATATTGTGTTCAGTTCAAGCTGTGAAGAAAAATATATATC AATGTTTTCCAATAAAATACAGTGACTACCAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC050926
Insert Size	450 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050926, AAH50926
RefSeq Size	2155 bp
RefSeq ORF	450 bp
Locus ID	12315
Gene Summary	Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2.[UniProtKB/Swiss-Prot Function]

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