Pnrc2 (BC006598) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC11707
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC006598 GGAAAGGCAGTAGGAGGTTGGTCCCCAGACGGAGTTGTGATCCGTGACTCGTTCGTGGGCTGTTAGATCC GTGCATCGTTTCTCCCTGTAGAGGGAAGGAGGCTTGGAGGTCGAGAGGGAGCCGCCGCCGCCGCCGCTCG AAGTCGCTAGGACTTCTCTCAAACTTGTGTGCTGAGGAGACTCGATGTGGACCTCAGCTCCTAGGCTGAA ATCAGCAGACTGGCTCATGAAAACTTGTGTACTGAGACAAAGGAAAGGATCTAGCAGAAAGCAACGTCTC TTATCCTGGGCTTGGCAGCAAGGAAGAGGACAAGTAGTGGAAATCCTTCAATCTGAAAAGCAAACTGAAA GGTGACAAAGAAGCTGAAGATGGGTGGCGGAGAGAGGTATAACATTCCAGACCCTCAATCTAGAAATGCT AGTAAGAACCAAGAACAGCAAAATAGACAGAAGAGCAAGGATCAGAATTCTTCCCAGACGAAGATTGCTC ATAAGAAAAAGGAACGAGGACATGGGTACAATCCAGCAGCAGCAGCATGGCAGGCCATGCAAAATGGGGG AAAGACCAAGAGCCTTTCTAACAACTCCAACTGGAATGCTGGTTTATCAAGTCCTAGCTTGCTTTTTAAG TCTCAAGCTAGTCAGAACTATGCTGGAGCCAAATTTAGTGAACCACCATCACCAAGTGTTCTCCCCAAGC CACCAAGCCACTGGGTTCATGTTTCCTTGAACCCTTCAGATAAGGAAACGATGACATTTCAACTTAAAAC CTTACTTAAGGTACAGGTATAAAGTTAGTAGGTGTTAACTTTAAATTGTGACGCTTACACATGACATCTG ATTTATGTGCTGTACACACTATACAGTGTCGATGCAATTTCAACAATTTAAGTGACTAATTGATTCTACT TGGTAAATTCAAGTTTAGATCTGGGAAATAGTAACCTTAACTTTTTTATTGTTTGATGGGAAAAATTAAG ATTTATGGGTTAACACACAACTTCTGAAGAAGTCAGACAGTGCATTTAGCATATACAAGGTTGCACAGTT CAGTTTCATGGTCTAGGGTCATCCAAACTATCTTGGGCATTTTTCAAAAGATAGCGGCGCAGCACATGTC CCAGTGGTCATTTCTTAAATGCAGTGGATAAGTACCCAGAGATGTACCAAGTCTATTCTAAAAACAGACA GTGCTGACTAGCACCCCACAAAGCTGCTAAAGTGATCCTGGCCTCACCTATATACAAAGCCTGTTTGAAG TAGGGAGTTGTGTTTGTGACATTTAAAGTCATGTAAATACATGTAATAAGCTTGTAACATATGTAATGTT TTCTTAGCCTTTATTCTACAAAAATAAGACTATTTTTAGTGTGAATTTGATGAATGTAAGACCATGAACT GTTTTTTTTTTAAAAATCTTATGGCCTAATTTTGAAGGTCTCCAATTTTTAAAGGTTGCCCTTGTGCTTA AGTGCCAGAGTGAATCAATGTAACTGATGTGTGAAATCTGTTGTATTAAGTTTTTATAATTGAAAAGATT TAAGCTACATTTCCTGTTTTTTTTTAAAGAGATATAAAGATGTAGGACAAAACTTCCTCCCCAACCATCT TAAACCCAAAGACTCATTTTGGCTTTATCAACCCTAAGTCACCCCTGCCTGATTATGTGCAACCCCTCAT TTTCATTGGCAAAGGGAAAAAGTCTCTTGTGTTATTAAACCCAGTAACAATTACTTCTCAATCTTTATGA GCAGAAGAGCTAAATTGTGTTTTCTCAATTAAGTTCAGAGCAAGAGTTCAAGTCAAACATTGAGTATCCT AAAGGACAAGTTCACAAAACTATAATCTGACTGAAAAATAAAATTCATAAATAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC006598
Insert Size	423 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006598, AAH06598
RefSeq Size	1889 bp
RefSeq ORF	423 bp
Locus ID	52830
Gene Summary	Involved in nonsense-mediated mRNA decay (NMD) by acting as a bridge between the mRNA decapping complex and the NMD machinery (By similarity). May act by targeting the NMD machinery to the P-body and recruiting the decapping machinery to aberrant mRNAs (By similarity). Required for UPF1/RENT1 localization to the P-body (By similarity). Plays a role in glucocorticoid receptor-mediated mRNA degradation by interacting with the glucocorticoid receptor NR3C1 in a ligand-dependent manner when it is bound to the 5' UTR of target mRNAs and recruiting the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (By similarity). Also acts as a nuclear receptor coactivator. May play a role in controlling the energy balance between energy storage and energy expenditure (PubMed:17971453).[UniProtKB/Swiss-Prot Function]

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