Isgf3g (BC012968) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	p48, Irf-9
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC012968 CCACGCGTCCGCTTATGAGCAAATGTTGCCTCTGTAGATGCTTGGGAGCTAAAACTCTGCCACAAGGAAC AAGTCATTAAGGGAATTCTCCTAAATAATACATGTCTGAAACACTTCAACTTATCAAGACCAAATGTCTG AGGTTTCCTTTCAGGATGGCCTCAGGCAAAGTACGCTGCACCCGAAAGCTGCGGAGCTGGATCGTGGAGC AGGTGGAGAGTGGCCATTTCCCAGGGGTGTGCTGGGACGATGCAGCCAAGACCATGTTCCGGATTCCCTG GAAGCATGCAGGCAAGCAAGACTTCCGAGAGGACCAGGATGCTGCCATATTCAAGGCTTGGGCACTGTTT AAGGAAAAGCACAAAGATGGGGACATAGGACACCCCGCTGTCTGGAAGACTCGCCTACGCTGTGCCCTCA ACAAGAGTTCCGAATTTGAGGAGGTTCCCGAGAGAGGTCGTATGGATGTTGCTGAACCCTACAAAGTATA TCGAATACTGCCAGCAGGAACCCTCCCTAACCAACCACGGAACCAGAAATCACCATGCAAGCGAAGTATC AGTTGTGTGTCACCTGAGAGGGAAGAAAATATGGAAAATGGGAGGACCAATGGCGTTGTAAACCACTCAG ACAGTGGCAGCAACATAGGCGGTGGTGGCAATGGCAGCAACAGGAGCGACAGCAACAGCAACTGCAACTC TGAGCTAGAGGAGGGAGCTGGCACAACTGAGGCCACCATTAGAGAGGACCCAGTGTTCCTGGAGCATCAA CTTCCTCTGAACTCAGACTACTCGCTGCTGCTCACCTTCATCTATGGTGGCCGAGTGGTGGGTAAGACCC AGGTGCACAGCCTAGACTGTCGGCTCGTGGCTGAGCGCTCAGACTCGGAGAGCAGCATGGAGCAGGTGGA GTTTCCCAAACCCGACCCACTGGAGCCTACCCAGCACCTGCTGAATCAGCTTGACAGAGGCGTCCTGGTG GCCAGCAATTCCAGAGGCCTCTTTGTTCAGCGCCTTTGCCCCATCCCCATCTCCTGGAATGCACCAGAGG CCCCACCCGGGCCTGGTCCTCATCTGCTGCCCAGCAATAAGTGTGTGGAGCTCTTCAAGACCACCTACTT CTGTAGAGATTTGGCCCAGTACTTCCAGGGCCAGGGGCCCCCACCCAAGTTCCAAGCAACCCTACATTTC TGGGAGGAGAGTCCTGGCTCTAGCCATAGCCAAGAGAATCTCATCACAGTGCAGATGGAGCAGGCCTTTG CCCGACATTTACTGGAGAAGATTCCAGAAGAGGAGAAAGCTGCCTTGTTCCTGTTACAGCACACAGAGCA GTCACCCTCTGCTCTGGGACACTGATCGTCGCGTCTCCATGGAAATAGACTTGTTCTCCACACTCTTGGT CGGCCTGCTTCTGTGATGGTTCTTATCACCGTGTGTTATCCAGCATGGCCAAGAGAAAGTGCAAGGGTGA TGTTTCTCCTTACAAATTGAGTTTGCGAGGCAGACTTGTTGAGGATACTTGTAATCTCAGCACTTGGAAG ACAGACCAAATGGTTATGAGCTCAAAGACAACCTGGAAGAGACTTTTATCTCCTTTTTTTTAAAGCTATA TTCTCTCTCAGCTCCAAGGAACTGGAAAGTTCCAAAGGGTGTGATCTCAGAGGGAATTGTATGCTCCTCC CAGTCCCAAACTCTAAATCTAAGCACTTTATATATTTTATCTCCCAGTCCTCACTAGACTGAAATAAAAT TTAGCTGGGTGAGGTATTACATAGTTGGCACATGTGAGACAAGAGGCAAGAAGACTGTGAGTTCAAATTC AAGGCCAGTCTTGGTGACACTGTCTCCAAAAAAAATAAATAAAAAGCTTCCTAAGAGCGGCTGGAGAGAT GGCTCGGTGATTAAGAGCACTGACTGCCCTTCTGAAGATCCTGAGTTCAAATCCCAGCAACCACATGGTT GCTCACACCTGTCTTCAGACACCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTATACTTACATATAATA ATAAATAACTCTTTGGGCCAGAGATCAGCCTCCATCCGCTCACATGTTGACTACTTGGTCCTCAGTGGAA CCACTTGGGATGACTAGAGGTGTGGGGTTGTTGGTTGAGTTGTGTCACTGGCCATGGGCTTTAAGGTTTC CAAAGACCCTTGCCATTCCTAGCTAGCACACTCTCTCTGTGTCTCATGCTTATAAGTCAGATGTAAACTC TCAACCTCAGCTCCATGCTTGCCTGCCTCCTGCTTTGTTCTCTGTTGTGATGGTCATGGACCCTAAACCT TTAGAAATGTGAGCTCCAAATTAAATTTTGCCTTTTCTAAGTTAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC012968
Insert Size	1200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012968, AAH12968
RefSeq Size	2368 bp
RefSeq ORF	1200 bp
Locus ID	16391
Gene Summary	Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.[UniProtKB/Swiss-Prot Function]

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