Ttl (BC018513) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410003M22Rik; 2700049H19Rik; AI848570
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC018513 CGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGGGCGCGGGCGCCCAGGGCAGAC ACTAGGGTAATCCGGAGGCGCGGCCCTGGAATTAGACCGACAGCGGGGCTGAGGCTGAGGCTGGGGCAGT GTGCACGCGGGGCCGCCGTGCCCTGGACTCGTGTAGCGGCTGCGGGGCGCGCTGCCCTTCGCCATGTACA CCTTCGTGGTGCGCGACGAGAATAGCAGCGTCTACGCCGAGGTCTCCCGGCTGCTGCTGGCCACCGGCTA CTGGAAGAGGCTACGGCGAGACAACCCCAGGTTCAACCTGATGCTGGGGGAGAGGAACCGACTGCCCTTC GGGAGACTAGGTCACGAGCCTGGGCTGGCACAGCTGGTGAATTACTACCGGGGGGCGGACAAACTGTGCC GCAAAGCTTCCTTAGTAAAGCTAGTCAAGACCAGTCCGGAACTGTCTGAGTCCTGCTCATGGTTCCCCGA GTCGTATGTGATTTATCCAACTAACCTCAAGACGCCAGTCGCTCCGGCGCAGAATGGCATCCAACTTCCG GTCAGTAACTCCAGGACAGATGAGAGAGAATTCTTCTTGGCTTCTTATAACAGAAAGAAAGAGGACGGGG AGGGCAACGTTTGGATTGCAAAGTCATCAGCTGGTGCCAAAGGTGAGGGCATCCTCATCTCCTCAGAGGC ATCGGAGCTTCTGGATTTCATCGACAGCCAGGGCCAAGTGCACGTGATCCAGAAATACCTCGAGCGCCCT CTGCTCCTCGAGCCCGGCCACCGCAAGTTTGACATTCGAAGCTGGGTCCTGGTTGACCATCAGTATAATA TCTACCTCTATAGAGAGGGTGTGCTTCGAACTGCTTCAGAACCATATCATGTTGATAATTTCCAAGATAA GACCTGCCATCTGACCAATCACTGCATTCAGAAAGAGTACTCAAAGAACTATGGGAAATACGAAGAAGGG AATGAAATGTTCTTCGAAGAGTTCAATCAGTACCTAACCAGCGCTCTGAACATCACCTTAGAAAGTAGCA TTTTGCTGCAAATCAAACACATAATAAGGAGCTGCCTCATGAGTGTGGAGCCCGCCATCAGCACCAAACA CCTCCCTTACCAGAGCTTCCAGCTCCTCGGCTTTGACTTCATGGTGGACGAGGAGCTGAAGGTCTGGCTC ATTGAAGTCAACGGCGCCCCAGCGTGTGCCCAGAAACTCTATGCAGAACTGTGCCAGGGCATCGTGGACA TAGCCATATCCAGTGTCTTCCCACCCCCGGACACGGAGCAGGTGCCGCAGCAGCCGGCTGCGTTCGTCAA GCTGTGAAGCACCGGAAGGCACAGAGCCACCTTGGGAAATGTACGGGGTCCTGCTCCAGAGGAAAGAGAG AGAACTGGACTGCTCCTTGTCAAGGCATGCTGGGGGACAGATGTGCAGGAAGAGTGTGAAGAGATGGCGG CTGTGGAAGAGCAGCTGAGCTGCCCACAGTGCTGTGGAGACCTTGGGAAGCTGGCGCAAGCGACTGAGAG GGAGAGCCCAGCACTTCTCTGAAGGGCTGGGACCAGGGATTTGTTATGGCGTGATCTGATCCTTTGAGCG GTTTCTGGCCTTGGATGGTACTGGGAACAGAGCGCCTTCTCCCCGGGATGCAGCCCAGGGGCCTCTGAGG GGGTGTCTGCTCACCTGCTGCAGCTTTAGTGCCTTAGTCTGTGCATGGTGCAGCCTCACTGGACACAGAC TGGACCTTAGAGCATTTTAGAGGTTGCCACATGTTCCCTGCGACACTATGTCCCGCTGAACTCTGGACCA GCATGCATCACAGTCCGGGCTGTGCCCTGGCCTGTCAGTGAAGGCCTCTTGCTTGTCACCATCCCGGTGG GCTATGAGTGAGCGCCTGCCAGGGGTCCTGTGGACGCCAGTGCATGCTGCTCACTGCATGTTTTAGAAAC AAAAGCCGCAGGCCTCACGAATCTGCAAGCATCAAATAAGCATGAGAGAGAGAAAAACAAAAGCAAAACC AAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC018513
Insert Size	1134 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018513, AAH18513
RefSeq Size	2049 bp
RefSeq ORF	1134 bp
Locus ID	69737
Gene Summary	Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.[UniProtKB/Swiss-Prot Function]

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