Pcgf6 (BC016195) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933407A11Rik; AI604840; MBLR; Rnf134
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC016195 AACTCGCCAGATTTCTGATCCCCGCCATGGACGAGGCTGAGACGGACGCCACGGAAAACAAGCGCGCCTC TGAGGCCAAGCGAGCCTCAGCCATGCCGCCACCGCCACCGCCTCCGCCTCCCATCTCTCCGCCCGCCCTC ATCCCGGCACCCGCGGCCGGCGAGGAGGGCCCGGCGTCCCTGGGCCAGGCGGGCGCTGCCGGCTGCTCTC GCTCTCGGCCCCCGGCGTTGGAGCCCGAGCGCAGCCTGGGCCGCTTGAGAGGCCGCTTCGAGGACTACGA TGAAGAGTTGGAAGAGGAGGAGGAGATGGAGGAGGAAGAAGAGGAGGAGGAAGAGATGAGCCACTTCTCT CTGCGTCTGGAGTCCGGACGGGCAGACTCCGAGGACGAGGAGGAGCGCCTGATAAACCTTGTCGAGCTGA CCCCCTACATCCTGTGTTCGATTTGCAAAGGTTACCTGATAGATGCAACCACCATTACAGAGTGTCTTCA TACCTTTTGTAAAAGCTGCATTGTGAGACATTTTTACTATAGTAACAGATGCCCAAAATGCAACATAGTT GTCCACCAGACTCAGCCTCTTTATAATATAAGGTTGGACCGGCAGTTACAAGACATAGTATACAAATTAG TGATCAATCTAGAGGAAAGAGAAAAAAAGCAAATGCATGACTTCTATAAAGAAAGAGGTCTAGAAGTCCC CAAGCCTGCTGCTCCACAGCCAGTCCCTTCAAGCAAAGGAAAAACCAAGAAGGTCCTAGAGTCAGTGTTT CGTATTCCACCTGAACTTGATATGTCTTTATTACTTGAGTTCATTGGTGCTAATGAAGACACCGGACATT TTAAGCCATTGGAAAAGAAGTTTGTGCGTGTTTCAGGAGAAGCAACTATCGGGCATGTAGAAAAATTTCT TAGAAGAAAAATGGGTCTTGATCCAGCTTGTCAGGTAGATATCATCTGTGGGGATCACTTACTGGAGCGG TACCAAACTCTACGGGAAATCCGACGTGCAATAGGTGATACAGCAATGCAGGACGGTCTGCTTGTTCTTC ATTATGGTCTTGTGGTTTCTCCTTTGAAAATAACTTGAAGATGCAAAGGTATATGAGGAGAGAAACCCAG TCGGAGGCATCTGCATATCTGCATTGCACCAAAGATTCTCACAAAGCACGTAACTACGACTACTTTCCGC TGTTAAGAGCTGGCTTCTGTTGTCACCAGAACCCTAGCATTGTGTGTGTACAACTTGAAACAATAGAATG TATTTGCTACACTAAAGACTATGGAAACAGCACTCATAACTCAGGGGAAAAAACTGAAAACACTGAATTT TAAAATTCCTTGTGATTCCAAATATGAGGAAGTTGCTGGTTGCTTCCCATCTTTGAGGTGGGTGGGTCAG TTTCCCAGAGCTGGGACTCTGCCCTCCAGCTGCATGGGCTGGGTGGTTTCCTTCAGAACAGCTTTGCTTT GAGTTCAGAGCCAGTCTGTTAAACCTCGGGTGCGCTCCGCACCGAAACTACTGGCCACTTACAAGTTCCT GTCCTATTCTGAACTTCAAACAAAATTCCACTGATACCACTAAAGCCCTGTTCCTCAGCTCTGAGGCTGA GACTGTCTCCTGTTAGAAATGGCTCTTCTTAACTAACATCCCTTAAACAAATATGAGGTGCTTCTTTCTG ATGGGACACTGGGTCAGGTGTCACAAAATTCCCTCAGTGTGGCTGTGAATGATGGCACTGCTTCTAACAG TAGCAACAAATTTCAGGTGAATGACCAGAAGTTTACAGAACCCTTATTGTTCCTCAGTCACTTAAATGAT CTGGTTCCATGTTAATGTTTCTGTTTTCTTGCCATTTGGATAAAGAATGAGAGTCTTTGAGAACCTTGCT TTCACAGAGACTTCATTGTAAACATTACTTTGCCTAAAATTAGCATCCTGATGCTTTCTAAAATACATAT TTTATAGACTTCATAGCAAAATTTCCAAATCTGTACAGAAATTTAAAAAATTAGACTTAAATTGGCGATT GTTTTTGTAAAGAATTTATGAACTGTACATAAATCTCTTCTGACATTTAATAAAAATTTATTTTAGCCCA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC016195
Insert Size	1062 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC016195, AAH16195
RefSeq Size	2127 bp
RefSeq ORF	1062 bp
Locus ID	71041
Gene Summary	Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.[UniProtKB/Swiss-Prot Function]

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