Edn1 (BC029547) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ET-1, preproET
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC029547 GCCGGGTCTTATCTCTGGCTGCACGTTGCCTGTGGGTGACTAATCACACAATAACATTGTTTAGGGCTGG AATAAAGTCAGAGCTGTTTACCCCCACTCTATAGGGGTTCAATATAAAAAGGCGGCGGAGAACTGTCCGA GTCAGAAGCGTTCCTGCACCGGCGCTGAGAGCCTGACCCGGTCTGCTCCGCTGTCCTTGCGCGCTGCCTC CCGGCTGCCCGCGACGCTTTCGCCCCAGTGGAAGGGCCACTTGCTGAGGACCGCGCTGAGATCTAAAAAA AAAACAAAAAACAAAAAACAAAAAAACCCAGAGGCGATCAGAGCGACCAGACACCGTCCTCTTCGTTTTG CATTGAGTTCCATTTGCAACCGAGTTTTCTTTTTTTCCTTTTTCCCCACTCTTCTGACCCCTTTGCAGAA TGGATTATTTTCCCGTGATCTTCTCTCTGCTGTTCGTGACTTTCCAAGGAGCTCCAGAAACAGCTGTCTT GGGAGCCGAACTCAGCACCGGAGCTGAGAATGGAGTGCAGAGCCCCCCTCCCAGCACACCCTGGAGACCC CGCAGGTCCAAGCGCTGTTCCTGTTCTTCCTTGATGGACAAGGAGTGTGTCTACTTCTGCCACCTGGACA TCATCTGGGTCAACACTCCCGAGCGCGTCGTACCGTATGGACTGGGAGGTTCTTCCAGGTCCAAGCGTTC CTTGAAAGACTTACTTCCCAATAAGGCCACAGACCAGGCAGTTAGATGTCAGTGCGCTCACCAAAAAGAC AAGAAGTGCTGGAATTTCTGCCAAGCAGGAAAAGAACTCAGGGCCCAAAGTACCATGCAGAAAAGCTTAA AAGACTCCAAGAAAGGAAAACCCTGTTCCAAGTTGGGAAAGAAGTGTATCTATCAGCAGCTGGTGGAAGG AAGGAAACTACGAAGGTTGGAGGCCATCAGCAATAGCATCAAGGCATCTTTTCGTGTTGCAAAGTTGAAA GCTGAGCTCTATAGAGACCAGAAGTTGACGCACAACCGAGCACATTGACTACAGAGCTCCCCCAGTGTTT TGGAAGTCATCCCTTATATAGTGCGAGCCCCATGGCCAGCTCTGCACTCTCCATGCTGGCTGGGATCTTA GCAAGACCATCTGTGTGGCTTCTACAGTTTCTTGTTCAGACGGGCAGGACCAGCATCCTTGATCCAAACA TTCCAAGAAAGGCTGAGGTGTTCCCTAGCCTGTCTGCGTCCGCTGGGAGCGAGTGCCTTTCTGCCTCTTC TTGCCGGTTGGGAATGACAGAGGACTTCTCAGAGAGCAGAGACACGATGCCATTCTAGAGTGGCATCACT CAGAGAGCCAGGAGATTCCACAGCAGGGCGGGGTTTCTGTAGAAAGTCCTTAGGGAGTGTTCGTGTCTGA CTCAGGCGCCTGGCACATTTCAGGGAGAAACTCCAAAGTCCATGCAAAAATTTTTCTGAGGAATGCACAA ATTGAAAACATACTCGAAGGACAAACACTTGAGTTTAAAAAAAAAAAAAAGACAATTTTTAAGTTGTAAA ATGCAAAACTCAAAGCAACTGTTACTACTGTACATTGGGATGTATTTCATGAATATGAGTCTACCTCACC TTTCTGCACTGTCATACACATTCTCACCTTTAATTATTGCCTCCCTGTATTCTCTCCTCTCCGCTATCCG GGTTCCCAGTCCCCTATACTGAATCCTTGCCTCACAGAAATTGAGTCAGAAGTAGCTGAAACATTGCCAT GCTAATCTGCTAGCTCTAATCTATGAGAAAAAAGGATGACAGAATCTGGCCCCTGCCCTTGACCTTGGGA AACACAATGGTTTAGAGTTGTTTGTGTACATGTTGAAAACCTGGTCTGTGCTTTTTAGCAGTAAAAATAT GTTCCCGTATGTATTGTAACTGGCTAATGGAAGAGGTTAGATTGAATCTTGATGTACTTATTTTTTTATA GATATTTATATTCAAACAATTTATTCCTTATATTTACCATGTTAAATATATGTCTGGGCAGGCCATATTG GTCTATGTATTTTTTAAAAATATATATTTCTGAATGAAATTGAGAACATGCTTTGTTTTGCCTGTCAAGG TAATGATTTTAGAAAATAAATATTTTTCCCTACTGTAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC029547
Insert Size	609 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC029547, AAH29547
RefSeq Size	2153 bp
RefSeq ORF	609 bp
Locus ID	13614
Gene Summary	This gene encodes a member of the endothelin family of peptides. The encoded preproprotein undergoes proteolytic processing to generate a peptide before secretion by the vascular endothelial cells. The mature peptide has various biological activities such as vasoconstriction, cell proliferation, stimulation of hormone release and modulation of central nervous activity. Mice lacking the encoded protein exhibit neonatal lethality accompanied with numerous craniofacial and cardiovascular defects due to disruption in cranial and cardiac neural crest cell patterning during early embryogenesis. [provided by RefSeq, Feb 2016]

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