Tmem55a (BC021435) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610319K07Rik; AI315591; AV001360
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC021435 CCACGCGTCCGCCCACGCGTCCGCTCAACCCGCGGCCCGCCGCGCTGTCGCTGCAGTCATGGCTGCTGAC GGGGTGGACGAACGTTCGCCTCTGCTGTCAGCATCCCATTCGGGAAATGTCACTCCCACAGCCCCGCCGT ACTTGCAGGAAAGCAGCCCCAGAGCTGAACTCCCACCTCCGTATACAGCCATCGCCAGTCCAGGAACAAG TGGTATTCCCGTGATCAACTGTCGTGTGTGCCAATCTCTAATCAACCTGGATGGTAAACTTCACCAGCAT GTGGTTAAGTGCACAGTTTGCAATGAAGCTACGCCAATCAAAACCCCCCCAACAGGGAAGAAATATGTTA GATGCCCTTGTAATTGTCTACTCATTTGTAAGGATACATCTCGGCGAATAGGATGTCCGAGACCCAACTG TCGACGCATAATTAACCTTGGCCCCGTAATGCTCATTTCTGAAGAGCAACCAGCCCAACCTGCATTGCCG ATCCAGCCAGAAGGCACAAGGGTAGTGTGCGGGCACTGCGGGAACACATTCCTGTGGATGGAACTGAGGT TCAACACTCTGGCAAAATGCCCACACTGCAAAAAAATCTCCTCGGTAGGTAGCGCCCTTCCCCGGAGACG CTGCTGTGCGTATGTCACCATCGGAATGATATGTATTTTCATTGCAGTTGGATTAACTGTTGGCACACAA GATTTTTCAAGGCGATTTCATGCAACCTATGTGTCTTGGGCAATTGCTTATCTGCTAGGTTTGATTTGCC TTATCCGAGCTTGTTACTGGGGCGCAATCAGAGTCAGTTATCCAGAGCACGGCTTTGCTTAAGTCTCCTC ATGAATCCTTGATGCAGGCGAGAGTGTCCAGTAGTCCTTGAGAAGCTCTGCTGGACGCCTTCAAATCATT TATCCTCCTGGATTCCATCCTGGTTTGTATAGTAGTCTCAAGAATTTGTGAGTCTTTTCTGAACAGACGC TCACTGAAGTACATTATATGCAAGTTCATTTTGCTGCTAGGTTTTCAGACTTGGAAAAATAAAGCTGTGT CTCTGTTCTTTTAAATCATAAAGGTATTTTTGCATTTGTCCTCCCACGTTATATCTAATATCAGTCTTTC ATTATGTTTAAACCAGTTGTTCCATTACTTGTTGCTGTGGGACTATCCCCAAGAAGTGTTTATAAATGTT TCTACAATAGGGTCACATTTATACTTACATTTTAAACAGAATTGCTTGCTTTAAAAATCTAAGCAATATG CATTTTTGCTTGAACTGCTTACTGTAATTTTAACCTAAGAGTATAGATACCTTATTCAGGAAAAACAAAT TGAGTGTACTTTGAAAAACTTGACATTCTTGTCAGAGAATCATTTCTAGATAGTATATGCTTGTTTTTTT TGTTTTTTTTTTTTTTTGTTATTGTTTGTAGAAAGATACAATATTTTGGTAGTAAATTAAAATACCTAGG ATGAACATATATATAGTCCACAGTTGGAGATGTTAGATAAATGTCTTTTCTCTAAGATTACAGGACTTTT TGCCTTTCAGTCTTGTCTTTTTATTTAGCATTTACAAAAATCTGGTCTGGATTTATGGAATTTAATGTTA CTCTGCTGAATGTATTCCTTTATAAACTGGAGGAAGTATTTCAGACAACATGTTTTATAATACTTCATCA TCTCGTCAAAGACATGTTGACACTGGGTTGCAGTCCTTTTCATTCAATCATGGTAAACTTATCTTCTTGA CATCTGTGTACACCCGCTTCTCTGGGAAGAGAACTTAGGACTTTTTTGCTTTAGCTGAAAGTTAGAATTA GAGATTCTCTTACCATTTCTCACCTGGTCAGATAATGACTCGACTTCTTCAAGAGAACGGATCAGAGAGA GAGAGTTTCATCGTCACCTAAAACTTCGCGTGGAATTCATGGACTCAGCTCTTGCACTGCCCAGTTTTGC AGTTTAGAAGGAATTCCTTGAGAGTCAGTGGTCTAAACTCTTTCACATTGTAGAATTAGACAATGAGATG CCCTGAATACATTAGTAGGTGATTCCAAGGGACAAGACTAATGTTCTAAATTATTTATGTTCCTGATTAA TATGAAAATGTACTTAATGCAAAGTCGGAGTCAGCTACACCTCAAAGAGAAAATGATAAATGTATAAATG AAGTAGCTCAGGATTCTATGTACCTTTGAAAATATGCTAATAAAGATTATTATTCAAAAAAAAAAAAAAA AAAAA
Restriction Sites	RsrII-NotI
ACCN	BC021435
Insert Size	774 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021435, AAH21435
RefSeq Size	2245 bp
RefSeq ORF	774 bp
Locus ID	72519
Gene Summary	Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P) (By similarity). Does not hydrolyze phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-bisphosphate, inositol 3,5-bisphosphate, inositol 3,4-bisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate (By similarity). Negatively regulates the phagocytosis of large particles by reducing phagosomal phosphatidylinositol 4,5-bisphosphate accumulation during cup formation (PubMed:29378918).[UniProtKB/Swiss-Prot Function]

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