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Bub1b (BC031577) Mouse Untagged Clone
CAT＃: MC206387
Bub1b (untagged) - Mouse budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae) (cDNA clone MGC:36092 IMAGE:4953223),, (10ug)
CNY 7,336.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BUBR1, MGC36092 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC031577
CGGACGCGTGGGGCATGATGGGGCGTGAGGTGGCTATTCTGAGAAGGAGTCGGATGCGTGGTTTTGTTTA GGAGCACGGGGTGACGATCTTCGCAGCTCCAGAGCCAGCTGAAGGTTGAGGGAGGCTCAGTTAGCACTCG GAGGATAAAGGTCTGCGGAAGGGCGAGAGCAAGTATTGGGAATGCAGGATGGCGGAGGCGAGTGAAGCCA TGTGCCTGGAGGGAGCAGAGTGGGAGCTGAGTAAAGAAAACATACAGCCCTTACGGCACGGGCGGGTCAT GTCCACACTTCAGGGAGCTTTGGCAAAGCAAGAGTCAGCTGGCCACACTGCTCTGCAGCAGCAGAAACGG GCATTTGAATCTGAAATCCGCTTTTACTCTGGAGATGACCCTCTGGATGTGTGGGACAGATATATTAATT GGACAGAACAGAACTACCCTCAAGGGGGGAAGGAGAGTAACATGTCAGCGTTAGTGGAGAGAGCGATAGA AGCACTCCAAGGAGAGACGCGCTATTATAATGACCCCCGCTTTCTCAGTCTCTGGATCAAATTGGGACAT TTGTGCAATGAACCTTTGGATATGTACAGCTATTTACAAAGCCAAGGAATTGGCGTTTCCCTTGCCCAGT TCTATATTTCATGGGCTGAAGAATACGAAGCTAGAGAAAATTTCAAGAAAGCGGACATAATATTTCAGGA AGGGATTGAACGCAAGGCTGAGCCCCTGGACAGACTGCAGTCCCAGCACAGACAGTTCCAGTCTCGAGTG TCGCGACAAGCTTTCTTGGCCCTTGGGAATGAAGAGGAGGAGGCTTTGGAGCCTTCTGAACCACAGAGAA GCTCGCTAGCTGAGCTGAAGAGCAGAGGGAAGAAGATGGCCAGAGCGCCCATCAGCCGTGTCGGAAGTGC TCTGAAAGCTCCAGGTCAGAGCAGAGGATTCCTAAATGCAGTTCCCCAGCCAGTACACGGTAATCGCAGG ATCACCGTTTTTGATGAAAATGCCGATACCGCGTCTAGACCGGAGTTATCTAAGCCTGTAGCCCAGCCAT GGATGGCACCCCCTGTGCCCAGGGCCAAAGAGAACGAACTTCAGCCAGGCCCATGGAGCACAGACAGGCC GGTGGGACGCAGGCCTCATGACAATCCAGCCTCTGTGACGTCGATACCCAGCGTGCTTCCCAGCTTTACG CCGTACGTGGAAGAGAGCGCCCAGCAGACAGTCATGACACCATGCAAGATTGAGCCTAGTATCAACCATG TTCTCAGCACCAGGAAGCCAGGAAGAGAAGAAGGAGACCCCTTGCAGAGAGTTCAGAGTCATCAGCAAGG CTGTGAGGAGAAGAAGGAGAAGATGATGTACTGTAAGGAGAAGATCTATGCCGGAGTTGGGGAGTTCTCC TTTGAGGAGATCCGAGCTGAAGTGTTCCGAAAGAAGCTGAAAGAACGAAGGGAAGCCGAGCTGTTGACCA GTGCAAAGAAGAGGGAGGAGATGCAGAAGCAGATCGAAGAGATGGAGAGGAGGCTGAAGGCAATGCAGGC TGTTCAGCAAGAAGGAGCTGGTGGCCAGCAAGAAGAGAAGATGCCTACAGAGGACCCAGCCAGATTGCAG ATTGCTTCGGGGCCTCAGGAAATGTCGGGAGTTCCTCTGTCCTGTTCCATCTGTCCACTAAGCTCGAATC CTAGGGAAATTTCACCTGCTGAGAACATTTTGCAAGAACAGCCTGATTCTAAAGGTTCCAGTATGCCTTT CTCCATTTTTGATGAGTCTCTTTCAGACAAAAAAGACAAAAGTCCTGCTACAGGTGGTCCACAGGTTCTC AATGCCCAGAGAAGACCCCTTTCAGTTCTCAAAACTACAGAAGTGGGCACCACAAATGAGGATGTGTCTC CCGATATTTGTGATGAACTCACAGAACTTGAGCCTCTGAGTGAAGACGCCATCATCACTGGTTTCAGGAA CGTCACTCTCTGTCCCAACCCTGAGGACACTTGTGACTTTGCTAGAGCAGCTCGTTTGGCATCTACTCCT TTCCATGAGATACTGTCCTCGAAGGGCATCGCTGCTGATCCCGAGGGACTGTTGCAGGAAGAGGATCTGG ATGGGAAGGCCGCCGAGGCTCATCACACTGTTCATCACCAGGCCCTCATCATAAAGAAACTGAGCCCAAT TATTGAAGACAGCCGTGAGGCCACCCACTCATCTGGCTTCTCCAGGTCTTCTTCCTCAGCTCCCAGTACA TCCTCCATCAAAGGCTTTCAGCTTCTGGAAAAGCTGGAGCTGACTAATGACGGGGCAGAAAATGCTATTC AGTCACCCTGGTGTTCACAGTATCGCCTACAACTGTTAAAATCCCTACTAGAGATAAGTGCTTTTGCGGA GTTTTCTGTGGAAGACCGACCGATGCCTGTGCTGGAAATAGGGAAGGAGATTGAGTTAGGTCCTGAGGAT TACGTCATCAAGCAAGAGCACCTAACATGTGACGATTACAGGTTATTCTGGGTGGCACCAAGAAGCTCTG CAGAGCTAACCATGATAAAGGCATCATCTCAGCCTATCCCGTGGGATTTTTATATCAACCTCAAGTTGAA GGAGCGTCTGAATGAGGACTATGACCAGCTTTGCAGCTGCTGTCAGTACCAAGATGGCCATGTTGTTTGG TACCAGTATATAAACTGCTCCACCCTTCAGAATCTTCTCCAACACAGCGAATTTGTTACTCATGAAATAA TAGTGTTGATTATTTACAACCTCTTGACAATCGTGGAGAAGCTACACAGAGCTGAAATAGTGCACGGAGA CTTGAGTCCACGGAGTCTGATCCTACGAAACAGAATCCACGACCCCTATGACTATGTAAATAAGGACGAT CACGCTGTGAGGATCATGGACTTCTCCTACAGTGTTGACCTGAGGGTGCAGCTGGATGCGTTTGCCTATA GTGGCTTTCGGACTGCACAGATCCTGGAAGGACAAAAGATCCTGGCTAACTGTTCTTCTCCCTACCATGT AGATCTGTTGGGTATAGCAGACCTAGCGCACTTACTCCTGTTCAAGGAGCACCTCCATGTCTTCTGGGAT GGACTCCTCTGGAAACTTAGCCAGAGCACCTCTGAGCTAAAAGACAGTGAATTGTGGAATAAATTCTTTG TGCGGATTCTGAATGCCAGTGACAAGTCCACAGTGTCTGTTCTGGGGGAGCTGGCAGCAGAAATGGGTGG GGCTTTTGATGCCACATTCCATAGCCACCTGAACAGAGCCCTGTGGAAGCTGGGGAAGACAATCAGCCCG GAAGCTTTGCTCACTCAGCAAGACAAGCAGCCAGGCGGCTCCCAGAGCCCTGCCTAAGCCATGCGAATGG TGAAGAACTGGTCTGGGGCTGCTGTGATTTGTTTAGAGCACACTTAGACACATCAGCAGTCCTTCACTAG GGGTCATTCTCAGTAGCACAGCTTTCAGCCAGGTGGCTGTGCCCTGGCAGCCTCATCTGGTTTATTCAAA AGGTTTCTGTGCAGACAGCTGCTTTTTAGCCCTCTGGCTTCTGCCTTCTCTCTTTTGTAATTTTATAACA TGTACTCTCCTTTCACTGTGTATTTTATAATAAAATAGTTTTTGTTAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC031577 |
| Insert Size | 3159 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031577, AAH31577 |
| RefSeq Size | 3631 bp |
| RefSeq ORF | 3159 bp |
| Locus ID | 12236 |
| Gene Summary | Essential component of the mitotic checkpoint. Required for normal mitosis progression and tumor suppression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. Essential for tumor suppression. May play a role in regulating aging and fertility (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Prediction of drug candidates for clear cell renal cell carcinoma using a systems biology-based drug repositioning approach
,null,
EBioMedicine
,PubMed ID 35339898
[Bub1b]
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