Poll (BC004767) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110003P06Rik; AV007317
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC004767, the custom clone sequence may differ by one or more nucleotides ATGGACCCTCAGGGCATCGTGAAGGCCTTTCCCAAGCGGAAGAAAAGTCATGCAGATCTATCTTCAAAAG CACTTGCAAAGATTCCCAAAAGGGAGGTGGGAGAAGCTAGAGGATGGCTGAGCTCCCTGAGAGCCCACAT TATGCCCGCTGGCATTGGACGTGCCCGGGCTGAACTCTTTGAGAAGCAGATTATCCACCATGGCGGCCAG GTGTGCTCTGCCCAGGCCCCAGGAGTCACTCACATTGTGGTGGATGAAGACATGGACTATGAGCGGGCTC TCCGGCTCCTCAGGTTGCCCCAGCTACCCCCTGGTGCCCAGCTGGTGAAGTCCACCTGGCTGAGCTTGTG CCTGCAGGAGGGAAGGCTGACAGACACGGAAGGATTCAGCCTTCCCATGCCCAAGAGGTCCTTGGATGAA CCACAGCCCAGCAAGTCAGGCCAAGATGCTTCTGCTCCTGGCACCCAGCGGGATTTACCCAGGACCACCC TTTCTCTTTCCCCTCCTCACACCAGAGCTGTATCTCCTCCCCCAACGGCAGAAAAGCCATCAAGAACCCA AGCCCAGCTCAGCTCAGAGGATGAAACCAGCGATGGAGAAGGGCCCCAGGTTAGCTCAGCAGATCTGCAA GCCTTGATCACTGGGCACTACCCCACTCCCCCTGAGGAAGATGGTGGGCCTGACCCAGCACCAGAAGCTC TGGATAAATGGGTCTGTGCACAGCCCTCGAGCCAGAAGGCAACTAACTACAATCTGCACATCACAGAGAA GCTGGAAGTGCTGGCTAAAGCCTACAGTGTCCAGGGAGACAAGTGGAGGGCCCTGGGCTATGCAAAGGCC ATCAATGCTCTCAAGAGCTTCCACAAGCCTGTCAGCTCCTACCAGGAGGCCTGTAGCATCCCGGGAATTG GCAAGCGGATGGCGGAGAAGGTCATGGAGATCCTGGAGAGTGGGCATCTGCGGAAGCTAGACCACATCAG TGACAGTGTGCCTGTCTTGGAGCTCTTCTCCAACATCTGGGGAGCTGGGACGAAGACTGCCCAGATGTGG TACCATCAGGGCTTCCGAAACCTAGAAGATCTCCAAAGCCTGGGCTCCCTGACCGCTCAGCAGGCCATTG GCTTGAAGCACTACGATGACTTCCTGGACCGCATGCCCAGGGAGGAGGCTGCGGAAATTGAGCAGACGGT CCGGATATCAGCCCAGGCCTTCAACCCTGGGCTGCTGTGTGTGGCTTGTGGCTCTTACCGACGGGGGAAG ATGACGTGCGGGGATGTGGATGTACTCATTACTCACCCCGATGGCCGATCCCACCGGGGCATCTTCAGCT GCCTCCTTGATAGCCTTCGGCAGCAAGGGTTCCTCACAGATGACTTGGTGAGCCAGGAGGAGAACGGCCA ACAGCAGAAATACCTGGGTGTGTGCCGGCTCCCAGGGCCCGGGAAGCGCCACCGGCGACTGGACATCATC GTGGTACCCTACTGTGAGTTTGCCTGTGCCCTGCTCTACTTCACCGGCTCTGCCCACTTCAACCGGTCCA TGAGAGCTCTGGCCAAGACCAAGGGCATGAGCCTGTCGGAGCATGCGCTCAGTGCAGCTGTAGTCCGGAA CAGCCAAGGTGTCAAGGTGGGGCCTGGACAAGTGCTGCCCACCCCCACGGAGAAGGACGTCTTCAAGCAC TTAGGCCTGCCCTACCGGGAGCCAGCTGAACGGGACTGGTGA
Restriction Sites	EcoRI-NotI
ACCN	BC004767
Insert Size	1722 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004767, AAH04767
RefSeq Size	2315 bp
RefSeq ORF	1722 bp
Locus ID	56626
Gene Summary	DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.[UniProtKB/Swiss-Prot Function]

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