Kpna1 (BC006771) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mSRP1, NPI1, IPOA5
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC006771 GCGAGGCCTGGTGAGCGCCGCCGTGGTGCGCGCCGGCTGGTCAAGGTTGTGCGCAGAAGAGGCGCAGCGG GCGGGCCAAGAGCGAGCGTCTCCCTCTTCGTAGTGGCACCGGGACCGGTCGCCTGGAGCCGAAATCATGT CCACACCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAATCCTGATGAGATGCG CAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGGAAGCAGAAAAGAGAAGAGCAGTTATTCAAGAGAAGA AATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAAGTTATGTCTGATGGTGGCTTTCATGAGGCCCAGA TAAATAACATGGAGATGGCACCAGGGGGTGTCATCACCTCTGACATGACTGATATGATATTCTCGAATAG CCCAGAGCAGCAGCTCTCTGCAACACAGAAGTTTAGGAAACTACTTTCAAAAGAACCTAATCCTCCAATT GATGAAGTCATCAACACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAAAATTGTA CACTGCAGTTTGAATCAGCTTGGGTCCTGACAAATATTGCTTCAGGAAACTCTCTTCAGACCCGGAATGT GATTCAGGCAGGAGCTGTGCCTATTTTCATCGAGTTGCTTAGCTCAGAGTTTGAAGATGTCCAGGAGCAG GCAGTTTGGGCTCTTGGCAACATTGCTGGAGATAGTACCATGTGCAGGGACTATGTCTTAAACTGCAATA TCCTTCCTCCTCTTCTGCAGTTATTTTCAAAGCAAAACCGCTTGACCATGACCCGGAATGCAGTATGGGC TTTATCTAATCTCTGTAGAGGGAAGAGCCCACCTCCAGAATTTGCTAAGGTTTCTCCCTGTCTGAATGTC CTTTCTTGGTTACTGTTTGTCAGTGACACTGATGTACTAGCTGATGCTTGCTGGGCTCTCTCATACCTGT CAGATGGACCCAATGATAAAATCCAAGCAGTCATCGATGCAGGAGTCTGCAGGAGGCTTGTGGAACTTCT GATGCATAATGACTATAAAGTAGTTTCTCCTGCTTTGCGGGCTGTGGGAAACATTGTCACAGGGGATGAT ATTCAGACACAGGTAATTTTGAATTGCTCAGCTCTTCAAAGTTTATTGCATCTGCTGAGTAGCCCAAAGG AATCTATCAAAAAAGAAGCCTGTTGGACAATCTCTAATATTACAGCTGGAAATAGGGCACAGATCCAGAC TGTGATAGATGCTAACATGTTCCCAGCTCTTATTAGTATATTGCAAACAGCAGAGTTCCGGACAAGGAAG GAAGCTGCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAGCAGATCAAGTACCTAGTAGAAC TGGGTTGTATCAAGCCACTCTGTGATCTCCTCACTGTCATGGATGCAAAAATTGTACAGGTTGCCCTAAA TGGCTTAGAAAACATCCTGAGGCTTGGAGAACAAGAAGCCAAACGAAACGGCTCAGGCATTAATCCTTAC TGTGCTTTGATTGAGGAAGCATATGGTCTGGATAAAATTGAGTTCTTACAGAGTCATGAAAACCAGGAGA TCTATCAAAAGGCTTTTGATCTTATTGAGCATTACTTTGGGACCGAAGATGAGGACAGCAGCATCGCACC CCAGGTTGATCTTAGCCAGCAGCAGTACATCTTCCAGCAGTGTGAAGCTCCTATGGAAGGTTTCCAGCTT TGAAGCAATCCTCTCCTTCTTGTTCCTGTGTCAGACCAGGCTATCCAGTCAAGTCCTCTTGTGGAGCCCA CAGTCCTCATGGAGCTAATCTCTCAAATGTTTTCCATAATACTGTTTGCGCTCATTTGCTTGCCTTGCGC ACCTGCTCTCTTACACACATCTGGAAAACCTCTGGCTCTCTGTGGTGGAATACCCTTTTATTACAAGGGT AACCAGAACGGCCCACTCTCTTATGGAAAAATCCCTAAGCTTTGGAGATCCGCACTTACATTAGAGTCAT GGGAATATATACACATACTAATGTGGCTCCCTTTTTTGTGGGGGAAAAAAGAGGACTCTTCCTCATTCCG TAAAGTGGGGAAAATGACATTGAAAGATAAAACTAAAACCTTTATCTTGAATTTTACACAACTACAAGGG AGATATTTAGACTTGTGTATACGTCAGAGTACTCTTCTCGAGTTCTTCCACAGTGAATCAACCCTTGGAG TACCTGGTGGCTTTTTCTAGCCACATTTGCATTAATCCTTATTGAGATGGGATGGTTTTCTTTCCTCTAA TGGAGTCATTTTCTTCAGATATTAAAGTTAAACCATCCAATCCCTCACCTTCAGCCTTCAGTGAATGTGC TTCTAGTTGTCAGGAATGCTGAAGAATTAACACTTTGACTTTTAAATGTGATCCTGGCTTGGCAGATTTC CTTAAAGCAGGAAGGAGAGAAGCATATATTAGTTTGTATGGCTTTTGCTTCTCTTTGTTTTTGTTTCTTA GGATTTGGAAGTGGTTCAGTTTTAACGCTGGTATGAAGGCTAAAAGTCCTTAAGTAAAACCATGTTCAAA AATCCAATTAAAGACTAAGAACATAAAACCCATCACTTTGTTTAAACCGGAAAAAAAAAAAAAAAAAAAA AAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC006771
Insert Size	1617 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006771, AAH06771
RefSeq Size	2667 bp
RefSeq ORF	1617 bp
Locus ID	16646
Gene Summary	Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.[UniProtKB/Swiss-Prot Function]

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