Samhd1 (BC012721) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E330031J07Rik; Mg11
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC012721 CCACGCGTCCGCTGCGCGCGAGCCGGTGCCGCGGTACTTAACTTCGCAGCCCCGGGTGTCGGAGGTAGCC ATGCAGAGCGCACCCTTGGAGCAGCCAGCTAAGCGACCCCGCTGCGATGGCAGCCCAAGGACGCCACCGA GCACTCCTCCTGCAACAGCTAATCTGTCTGCAGACGACGACTTCCAAAACACCGACCTCCGAACCTGGGA ACCGGAGGACGTGTGCTCCTTCTTAGAGAATCGTGGTTTCCGAGAGAAGAAAGTGCTGGACATCTTCAGA GACAATAAAATCGCCGGCTCGTTTCTGCCCTTTTTGGATGAGGATCGTCTGGAAGATCTGGGAGTAAGTT CCTTGGAGGAGAGGAAGAAGATGATAGAATGTATCCAGCAGCTGAGTCAGTCTCGGATTGATCTAATGAA GGTATTTAATGATCCCATTCATGGCCACATTGAGTTCCACCCTCTCCTTATCAGAATCATCGACACACCT CAGTTCCAGCGACTTCGCTATATCAAGCAGCTGGGGGGCGGCTACTATGTTTTCCCTGGAGCGTCCCACA ATCGCTTCGAACACAGTCTCGGAGTGGGGTACCTAGCAGGCTGCCTGGTGCGAGCACTTGCCGAAAAACA GCCAGAGCTACAGATCAGTGAGCGAGATATACTCTGTGTTCAGATTGCGGGGCTCTGCCACGACCTAGGT CATGGGCCATTTTCTCATATGTTTGATGGAAGGTTTATCCCACGGGCTCGCCCAGAGAAAAAGTGGAAGC ACGAACAGGGCTCCATTGAGATGTTTGAGCATCTGGTCAATTCTAATGAACTCAAACTTGTCATGAAGAA CTATGGTCTCGTCCCTGAAGAAGACATTACCTTTATCAAGGAACAAATTATGGGACCACCTATAACACCA GTCAAAGATTCCTTGTGGCCGTATAAAGGCCGCCCTGCCACGAAGAGCTTCCTTTACGAGATAGTGTCTA ACAAGAGGAATGGCATCGACGTAGACAAATGGGATTATTTTGCCAGAGACTGTCACCATCTTGGAATCCA AAATAATTTTGATTACAAGCGCTTCATTAAGTTTGCCCGTATCTGTGAAGTGGAGTACAAGGTCAAGGAG GACAAGACCTACATCCGTAAGGTGAAGCACATTTGTTCGAGAGAAAAGGAGGTTGGAAATCTGTATGACA TGTTCCACACTCGCAACTGCTTACACCGAAGAGCTTACCAACACAAGATCAGCAACCTCATCGACATAAT GATTACAGATGCCTTCCTCAAAGCAGACCCCTACGTGGAGATTACAGGGACTGCCGGGAAGAAGTTCCGC ATTTCCACAGCCATTGATGACATGGAAGCCTTCACTAAGTTGACGGATAACATTTTTCTGGAGGTTTTAC ACTCTACTGATCCACAGTTGTCTGAGGCCCAAAGTATTTTAAGGAACATTGAATGCCGCAATCTATACAA GTATTTGGGTGAGACCCAGCCAAAGCGTGAGAAGATTAGGAAGGAAGAATATGAACGGCTTCCCCAAGAA GTTGCTAAAGCCAAACCTGAAAAAGCCCCGGATGTTGAACTGAAGGCCGAAGATTTCATAGTTGATGTTA TCAATGTGGATTATGGGATGGAAGACAAGAATCCAATCGATCGTGTTCACTTCTATTGTAAGAGCAACAG CAAGCAAGCGGTCAGGATCAATAAAGAGCAGGTGTCACAACTGCTGCCAGAGAAATTTGCAGAGCAGCTC ATTCGGGTGTACTGTAAGAAGAAAGACGGGAAGAGCCTGGACGCCGCCGGGAAGCACTTTGTTCAGTGGT GTGCGCTCAGGGACTTCACCAAGCCACAGGATGGTGACATTATAGCTCCACTCATAACACCTCTGAAATG GAATAATAAGACTTCATCTTGCCTCCAAGAAGTATCCAAAGTAAAAACATGTCTAAAATTTTAAAAAAAA AAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC012721
Insert Size	1884 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012721, AAH12721
RefSeq Size	1967 bp
RefSeq ORF	1884 bp
Locus ID	56045
Gene Summary	Isoform 1: Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks (By similarity). Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells (PubMed:23972988, PubMed:23872947, PubMed:26667483, PubMed:29379009). Likewise, suppresses LINE-1 retrotransposon activity (PubMed:26667483). In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools (By similarity). Phosphorylation at Thr-634 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks (By similarity). Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication (By similarity). Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation (By similarity). Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity (By similarity). Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (PubMed:29669924).[UniProtKB/Swiss-Prot Function]

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