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F2rl1 (BC025432) Mouse Untagged Clone
CAT＃: MC206604
F2rl1 (untagged) - Mouse coagulation factor II (thrombin) receptor-like 1 (cDNA clone MGC:29183 IMAGE:5006769), (10ug)
CNY 3,656.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Par2, PAR-2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC025432
GTCCGCCCACGCGTCCGGGGAAGGGACCCTGTGCTCAGAGTAGGGCTCCGAGTTTCGAACCACTGGTGGC GGATTGCCCGCCCGCCCCACGTCCGGGGATGCGAAGTCTCAGCCTGGCGTGGCTGCTGGGAGGTATCACC CTTCTGGCGGCCTCGGTCTCCTGCAGCCGGACCGAGAACCTTGCACCGGGACGCAACAACAGTAAAGGAA GAAGTCTTATTGGCAGATTGGAAACCCAGCCTCCAATCACTGGGAAAGGGGTTCCGGTAGAACCAGGCTT TTCCATCGATGAGTTCTCTGCGTCCATCCTCACCGGGAAGCTGACCACTGTCTTTCTTCCGGTCGTCTAC ATTATTGTGTTTGTGATTGGTTTGCCCAGTAATGGCATGGCCCTCTGGATCTTCCTTTTCCGAACGAAGA AGAAACACCCCGCCGTGATTTACATGGCCAACCTGGCCTTGGCTGACCTCCTCTCTGTCATCTGGTTCCC CCTGGCCATTGCCTACCACCTACATGGCAACAACTGGGTCTATGGGGAGGCCCTGTGCAAGGTGCTCATT GGCTTTTTCTATGGCAACATGTATTGCTCCATCCTCTTCATGACCTGCCTCAGCGTGCAGAGGTACTGGG TGATCGTGAACCCCATGGGGCACCCCAGGAAGAAGGCAAACATCGCCGTTGGCGTCTCCTTGGCAATCTG GCTCCTGATTTTTCTGGTCACCATCCCTTTGTATGTCATGAAGCAGACCATCTACATTCCAGCCTTGAAC ATCACCACCTGCCACGATGTGCTGCCTGAGGAGGTATTGGTGGGGGACATGTTCAATTACTTCCTCTCAC TGGCCATTGGAGTCTTCCTGTTCCCGGCCATCCTTACTGCATCTGCCTACGTGCTCATGATCAAGACGCT CCGCTCTTCTGCTATGGATGAACACTCAGAGAAGAAAAGGCAGAGGGCTATCCGACTCATCATCACCGTG CTGGCCATGTACTTCATCTGCTTTGCTCCTAGCAACCTTCTGCTCGTAGTGCATTATTTCCTAATCAAAA CCCAGAGGCAGAGCCACGTCTACGCCCTCTACCTCGTCGCCCTCTGCCTGTCGACTCTCAACAGCTGCAT AGACCCCTTTGTCTATTACTTTGTCTCAAAAGATTTCAGGGATCACGCCAGGAACGCGCTCCTCTGCCGA AGTGTCCGCACTGTGAATCGCATGCAAATCTCCCTCAGCTCCAACAAGTTCTCCAGGAAGTCCGGCTCCT ACTCTTCAAGCTCAACCAGTGTTAAAACCTCCTACTGAGCTGTACCTGAGGATGTCAAGCCTGCTTGATG ATGATGATGATGATGATGATGATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCACCCGTGTGTGAGAG CGTAGTAGGAATGCACCAACATGCATGAGGCTGTCATTTCCTATCCAAGCTGCTGGTCTCTGCACCAATC ACAAGCATGCAGCTCTCCCCAAGATCGCCAGAAGCCTCCTCCTTTGCATGAGAACAGTCTTCCACTCTGA TGAAAAGCATCAGTATCAGAAACTGAAACAAACTGAGAGGAGCATGTTTTGTGGAAGTGAAGAGAGGATG GAGGGTCAGTGACTTGCAAAAAAAACCCAACCAAACAAAAACGACACCTGGCAAGAAGGCTAAGACTCTC TGAAATGCTTCCTTTTCCATCTGGAGTTCGTCACGGCTTTGTTCAGGACCTGAGGCCCTGGTAGAGCTTC AGTCCAGTTGATTGACTTTACAGACTTGAGAGAGGAATGAATGAGGAGTGAATGCGGCTCCTGGCGGCAT CCTAACCGGCTAACAGTGGCCTTGCTGGACAATAGGATTCAGATGGCTGGAGTTACATTCTCACACCATT TCATCAGAACTATTGGGGATCTTGATCAATGTGCAGGTCCCTTAGCGTCAGTAACCCTGGGAGCTCAGAC ACGATGGGGGTGAGGGTGGGGGTGGGGGTGGGGGTGAGGCTCTACAAACCTTAGTGATGACTGCAGACAC AGAACCATGGAGCTGAGCCTGCTTCTGCTTGCCAGGGCACCACTGTAATGTTGGCAAAGAAAAACCAACA GCAGTGTTTTGAGCCTCTTTTTTTGGTCAGTTTATGATGAATTTGCCTATTGGTTTATTGGGATTTTCAG TTCCTTTATTACTTTGTTGTAATTTTGTGTGTTTATTAGTCAAGAAAAAGAAGATGAGGCTCTTAAAAAT GTAAATAAAATTTTTGGTTTTTTGGTTTTTTAACTTGGGCCAACTACAAATACTGCTTAGGTTTTTTTCT AACTTAATTTTTAACTACATCATGTGAACTTAAGACATTTTCATGATAAAGCATTACTGTAGTGTCAGTT TTCCCTCATCCTCGATCATAGTCCTTCCCATGAAGCAGGGCCCTTCCCCTCCCCCCCCTTTGCCGTTTCC CTCCCCACCAGATAGTCCCCTGTCTGCTTTAACCTACCAGTTAGTATTTTATAAAAACTGATCATTGGAA TATTTATTATCAGTTTTGTTCACTGTTATCAGTTTTGTTCACTAATTTGTCCAATAATGGAATTAACGTC TTCTCATCTGTTTGAGAAAGATCTGAAACAAGGGGCCATTGCAGGAGTACATGGCTCCAGGCTTACTTTA TATACTGCCTGTATTTGTGGCTTTAAAAAAATGACCTTTGTTATATGAATGCTTTATAAATAAATAATGC ATGAACTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | EcoRI-NotI |
| ACCN | BC025432 |
| Insert Size | 1200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC025432, AAH25432 |
| RefSeq Size | 2772 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 14063 |
| Gene Summary | Receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Can also be transactivated by cleaved F2r/Par1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection. Generally is promoting inflammation. Can signal synergistically with Tlr4 and probably Tlr2 in inflammatory responses and modulates Tlr3 signaling. Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X. Regulates endothelial cell barrier integrity during neutrophil extravasation, probably following proteolytic cleavage by PRTN3 (By similarity). Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone; activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as GNAQ, GNA11, GNA14, GNA12 and GNA13, but probably not with G(o) alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. Believed to be a class B receptor which internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to GNAQ and GNA11; the function involves dissociation of Ripk1 and Tradd from Tnfr1. Mediates phosphorylation of nuclear factor NF-kappa-B RELA subunit at 'Ser-536'; the function involves Ikbkb and is predominantly independent of G proteins. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds; the function is independent of GNAQ and GNA11 and involves promotion of cofilin dephosphorylation and actin filament severing. Induces redistribution of Cops5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by Cops5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as proinflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immnune cells; activation enhances phagocytosis of Gram-positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses (By similarity).[UniProtKB/Swiss-Prot Function] |
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