Rasd1 (BC034166) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Dexras1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC034166 GACCAGCTCGCGCAGTCCCGAAGCCAAGCTCTTCCGTCACTCCGGAGTTCTCTGCAGCCCTCTACCTTCT CTCGGCCACGCATCCGCCCCTGGGGCCCCTCTACCCAATGAAACTGGCCGCGATGATCAAGAAGATGTGC CCAAGCGACTCTGAACTGAGTATCCCGGCCAAGAACTGCTACAGGATGGTCATCCTCGGCTCATCCAAAG TGGGCAAGACGGCCATTGTGTCGCGCTTCCTCACGGGCCGTTTCGAGGATGCTTACACCCCTACCATCGA GGACTTCCACCGAAAGTTTTACTCGATCCGCGGCGAAGTCTACCAGTTGGACATACTGGACACATCCGGC AATCATCCGTTTCCCGCCATGCGGCGCCTCTCTATCCTCACAGGAGACGTTTTCATTCTGGTGTTCAGCT TAGACAACCGCGACTCATTCGAAGAGGTGCAAAGGCTCAAACAGCAGATCCTAGACACCAAGTCCTGTCT CAAGAACAAAACCAAAGAGAATGTGGACGTGCCGCTGGTCATTTGCGGTAACAAAGGGGACCGGGACTTC TACCGGGAAGTAGAGCAGCGGGAGATTGAGCAGCTGGTGGGTGACGACCCTCAGCGTTGTGCCTACTTCG AGATCTCAGCCAAGAAGAACAGCAGCTTGGACCAGATGTTCCGTGCGCTCTTTGCCATGGCCAAGCTGCC TAGCGAGATGAGCCCCGACTTGCACCGCAAGGTATCTGTGCAGTACTGCGACGTACTGCACAAGAAGGCT CTGAGGAACAAGAAGCTTCTGCGTGCGGGCAGCGGAGGCGGGGGCGACCACGGCGATGCCTTTGGCATCT TGGCGCCCTTTGCTCGCAGACCCAGCGTGCACAGCGACCTCATGTACATTCGTGAAAAAACCAGTGTCGG CAGCCAGGCTAAGGACAAGGAGCGCTGTGTCATCAGTTAGGAGCCCCCAGGGTCAGCCACACAACCTGAG GACCTTTTTTGTTCAAAAGTCAAATCGGATTCCTGGACTAGCGGTCCGTGCCACAGGAGCGCCAATTGGC CTTCTCCTTCCCTCCCTGAGACCCAGCCCGGGGAGACCAAGCCAGTAAGGGACAGTCACCTGCTGTGAAA GGAAAGAGAACTAGCTAAGACAGGAGCTGTCACCTCCGATTCTGGGATGCCAGGACCCAGCAGAGGGGCG CTTGGCATTTTTCTCAAGAGGTTTGAGAAAAAGCGTGTGAAGTTCATCAGCCTGTGAGACTTAAAGTAAC TGTCCTGCTCGCTTCAGGGCAGGACTAATAATAGGGCATTATCTTGTCTGTGATTGGTGTTGCCATGGCC GCTGTCAGCCACCTCTGTCCTCAGCAAACTGGAAACTTTGGCTGGAGGTGGGCGGGGGGGGGGGGGGGGG TGTTCAATCATAGCCAAGTGACTTGTTTACATGTGTGTGTGCAATTACCCAAAAGAAAAACAAAACACAA AACTTGCACTTTAATAGTTCCAGTGTCAATGTGACATGAACAAAATCTCTACATTTCTATTGTGTGAGGT CTTTATTATTATTTTTTATTTAAAATAAAATAATTTAGAAACGGATAAAAAAAAAAAAAAAAAAAAAAAA AA
Restriction Sites	EcoRI-NotI
ACCN	BC034166
Insert Size	843 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034166, AAH34166
RefSeq Size	1612 bp
RefSeq ORF	843 bp
Locus ID	19416
Gene Summary	This gene encodes a member of the Ras superfamily of small GTPases and is induced by dexamethasone. The encoded protein is an activator of G-protein signaling and acts as a direct nucleotide exchange factor for Gi-Go proteins. This gene may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. [provided by RefSeq, Nov 2015]

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