Mapkapk5 (BC019184) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MK5, PRAK
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC019184 CCACGCGTCCGCCCACGCGTCCGCCGAGCTCCGAGCCCTTTGCTTCCTCGGCTGCCAGGGGACGGGACAG CCGCGCAGCCTCCGCCGCTTCGGGCTGTGGGAGCCCAGCGGAGCATGTCGGAGGACAGCGACATGGAGAA AGCCATCAAGGAGACCTCCATTTTAGAAGAATATAGTATCAATTGGACTCAGAAACTGGGAGCCGGAATT AGTGGTCCAGTTAGAGTCTGTGTGAAGAAATCCACTCAAGAACGGTTTGCACTGAAAATTCTTCTTGATC GTCCAAAAGCTAGAAATGAGGTGCGCCTGCACATGATGTGTGCCACACACCCCAACATAGTTCAGATTAT TGAAGTGTTTGCTAACAGTGTACAGTTCCCTCATGAGTCCAGCCCCAGGGCTCGACTCTTAATTGTAATG GAGATGATGGAAGGGGGAGAGCTATTTCACAGAATCAGCCAGCACCGGCACTTTACAGAGAAGCAAGCCA GCCAAGTAACAAAGCAGATAGCCCTGGCTCTACAGCACTGTCACTTGCTAAACATTGCGCACAGAGACCT CAAGCCTGAAAATCTGCTTTTCAAGGATAACTCTCTGGACGCCCCTGTGAAATTATGTGACTTTGGGTTT GCTAAAGTTGACCAAGGTGATTTGATGACACCCCAGTTTACCCCTTACTATGTAGCACCTCAGGTACTGG AAGCGCAGAGACGGCACCAGAAGGAGAAGTCTGGCATCATACCTACCTCGCCAACACCCTACACTTACAA CAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATAATTTATGTGATGCTGTGCGGATATCCTCCTTTTTAC TCCAAACACCATAGTCGGACTATCCCAAAGGATATGCGGAAAAAGATCATGACAGGAAGTTTCGAGTTCC CAGAAGAAGAGTGGAGCCAGATCTCAGAGATGGCTAAAGATGTTGTGAGGAAGCTTCTGAAGGTCAAACC AGAGGAAAGACTCACAATCGAGGGAGTGTTGGACCATCCCTGGCTCAACTCGACAGAGGCCCTGGATAAT GTGCTACCCTCTGCCCAGCTGATGATGGATAAGGCGGTGGTTGCGGGGATCCAGCAGGCGCACGCCGAGC AGCTGGCAAACATGAGGATCCAGGACCTCAAGGTCAGCCTCAAACCCCTGCACTCTGTCAACAACCCCAT TCTCAGGAAGAGGAAGCTGCTGGGCACCAAGCCAAAGGACGGTATTTATATACACGACCATGAGAATGGA ACTGAGGACTCAAATGTTGCCTTGGAAAAGCTTCGAGATGTCATTGCCCAGTGTATCCTCCCCCAGGCTG GTAAAGGAGAGAATGAAGATGAGAAGCTGAATGAGGTAATGCAGGAGGCCTGGAAGTACAACCGCGAATG CAAGCTCCTGAGGGATGCTCTGCAGAGTTTTAGCTGGAATGGCCGTGGATTCACAGATAAAGTTGACCGA TTGAAGCTGGCAGAGGTGGTAAAGCAGGTGATCGAAGAGCAGACCCTTCCCCACGAGCCCCAGTAGTAAC AGCTTAAAACTGTTTTTAACAATTGGAAAAATTATTCCTTAATGTAAAAAGTAATTTTTATGTAAATTAA TAAATCATAATTTCATTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC019184
Insert Size	1422 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019184, AAH19184
RefSeq Size	1691 bp
RefSeq ORF	1422 bp
Locus ID	17165
Gene Summary	Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3' UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.[UniProtKB/Swiss-Prot Function]

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