Timp4 (BC064046) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	TIMP-4
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC064046 GTTTGTTACGACAGGCAGACCCCACGAGCTCAGCTGGGGCTCTGCAGTGTCATGCCCTGGAGTCCCCTGG CTGCGCTGAGCTGGGCGCTGGTGCTGAGGCTGCTGGCTTTGCTGTGGCCCCCTGGACGGGGTGAGGCGTG CAGCTGTGCGCCTGCGCACCCCCAGCAGCACTTCTGCCACTCGGCTCTAGTGATACGGGCCAAAATATCC AGTGAGAAGGTAGTCCCTGCCAGCAAAGACCCTGCTGACACTCAAAAACTGATCCGGTATGAAATCAAAC AGATAAAGATGTTTAAAGGGTTCGAGAAGGCCAAGGATATTCAGTATGTCTACACGCCATTTGACTCTTC CCTCTGTGGTGTGAAGCTAGAAACCAACAGTCACAAGCAGTATCTTTTGACTGGCCAGATTCTCAGTGAT GGAAAAGTCTTCATCCATCTGTGCAACTACATTGAGCCCTGGGAGGACCTGTCCTTGGTGCAGAGGGAGA GCCTGAATCATCACTACCACCAGAACTGTGGCTGCCAAATCACCACTTGCTATGCAGTGCCATGTACCAT CTCAGCCCCCAATGAGTGTCTCTGGACGGACTGGCTGCTGGAACGGAAGCTCTATGGGTACCAGGCCCAG CACTATGTCTGCATGAAGCATGTTGATGGCATCTGCAGCTGGTACCGAGGCCACCTACACCTCCGGAAGG AGTACGTTGACATCATCCAGCCCTAGTAGGGACCAGGAACCACATCCTTGGAAGCATTCTGAAGACCAAG CCAGTTCTCTGTGGTCCTTTGACCATCACCACCTGCTGTGTTGCTGCCAGATAATGGAAAATGACAAGTG GATGGCCTGGCCAGCAAATGGAGGCTGTGGGGCATGTGACAGCTTGTGTCTGCTGTTCACATCTCCAGCT CACAACTTGTCAAGGGTTGGAAAGGTATCTTATCTGCCTTGCCCTCAGCCCCTCTTCCCTGTCTCCCAAA CCCTGTTAGTCTAGCTTATACCTATTACTGAGAGTTTTTATTCTGGCTTTGTTTAACTGGCTTTTCTTAG CTCATATGATAAGGGAGAAGCTGTGACTGACTGTCATAAAGTTGTACAACCTTGCGATGTGTGCTATGGT AGGTGGGCTGACTGTATCCTGAGTGGGCTGTTTGGCTGAAGTGAAAAGAACACACTAAAGCTCCAGATCT CCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC064046
Insert Size	675 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC064046, AAH64046
RefSeq Size	1221 bp
RefSeq ORF	675 bp
Locus ID	110595
Gene Summary	Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.[UniProtKB/Swiss-Prot Function]

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