Krt20 (BC057172) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	K20, CK20, KRT21
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC057172 CCAAGTCCAGGCTACAGGCGTTGCCTCCCTACATCCCAGATGGATTTCAGTCGTCAAAGTTTTCACCGAA GTCTGAGTTCCTCCTCACAAGGCCCAGCACTCAGCATGAATGGCTCGCTGTATAGGAAGGGGACTGTGCA GCGTCTGGGGGCTGCACCCAGCGTCTATGGTGGGGCTGGAGGCCATGGCACTCGCATCTCTGTCTCCAAA GCCGTAATGAGCTACGGGGGTGATCTCTCCAACGGATCGGACCTGTTTGGGGGCAATGGGAAACTGGCAA TGCAGAACCTGAACGACAGATTGGCAAACTACCTAGAAAAAGTGCGCTCCCTGGAGCAATCCAATTCCAG ACTTGAAGCCCAGATCAAGCAGTGGTACGAAACCAACGCGCCGAGCACCATCCGAGACTATAGCTCCTAC TACGCACAGATTAAAGAGCTGCAAAATCAGGTTAAGGATGCTCAAGTACAAAATGCCCAGTGCGTCCTGC AAATTGACAATGCTAAGCTGGCTGCAGAGGACTTCAGATTGAAGTTTGAGACTGAGAGGGGAATGCATAT AGCTGTGGAAGCTGATCTCCAAGGCCTGAGCAAAGTTTATGATAATCTGACTCTTCAAAAGACAGACTTG GAAATTCAGATTGAAGAACTGAACAAGGACCTGGCCCTCCTCAAAAAGGAACATCAAGAGGAAGTTGAGG TTCTTCGCCGGCAGCTGGGCAACAATGTCAACGTGGAGGTGGATGCTGCTCCAGGCCTGAACCTGGGAGA GATCATGAATGAGATGAGACAGAGATATGAAGTCCTGGCCCAGAAGAACCTGCAAGAGGCCAAAGAACAG TTCGAGAGACAGAGTCAAACTCTGCAGCAACAAGTCACAGTGAACACGGAGGAGCTGAAAGGATTCGAGG TTCAAGTCACGGAGCTGAGACGCACCTACCAGAACCTGGAGATAGAGTTGCAGTCCCACCTCAGCATGAA AGAGTCTCTGGAGCGCAACCTAGAGGACGTCAAGGCTCGTTACGCCAGCCAGTTGGCAGCCATCCAGGAG ATGCTGAGCTCCCTGGAGGCCCAACTGATGCAGATCAGGAGTGACACAGAACGCCAGAACCAAGAACACA ACATCCTTCTCGACATAAAGACCCGGCTTGAGCAGGAGATCGCCACCTACCGCCGCCTTCTGGAGGGAGA AGATATAAAAACTACAGAATACCAGCTGAGCACTTTGGAAATGAAGGATATCAAGAAAACCAGGAAGATT AAGACGGTCGTGGAAGAAGTGGTAGACGGCAAGGTCGTGTCCTCTGAAGTCAAGGAGATAGAAGAGAGTG TCTAACCAGCTGTCAAAGGGAGGCTGCTCCAGCTGTGGCCCGCTGAAGACAACACTGTGTAGTGGCTTTA AAGTGTGTGTGTGGGGGGGGGGGGGGGAGTCTATTTATCAAGCTGTGTAATTAAATATAAATGGTTCACT CCAGGGAGCTGTAAAGGGCAAGATTAAACCCAGCACAATCGTCCTCATTGCAAGGCACATCCATCACAGA CCAGTTCAGGTAGAGTAAAGCATTGGAGGAGTTCAAGCAAAGAGCAAATAAACCGCTCTCCTTTGGGGTC TTGTTTTTCTTGTTTCTGAATTGCTAAAAGCCCCGTTAGTGAACGGGTTCTCCAGGGAGGCAACTTCCCA GTTAATCTCATTGTCTCCTTGCTGCCTTTAAGCCTTTCCTGATGTCTCCCACTGCCTCTCAAAACTCAAC TTAGTCTTTGAATAATTTAAAACAAACTCCAATCACAAATGCTGAGGAGAATAAGAACACAGCACAAGGT GATTGACATGGGGTTCAGTTAAGAGCAAAACTAGCCTGGACGGGAATGTGTCTTCGTCTCATAATAAACT GCTTAACCATTAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC057172
Insert Size	1296 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057172, AAH57172
RefSeq Size	1919 bp
RefSeq ORF	1296 bp
Locus ID	66809
Gene Summary	This gene encodes a member of the keratin protein family and is found within a cluster of cytokeratin genes on chromosome 11. Keratins are cytoskeletal proteins that are preferentially expressed in epithelial cells. The encoded protein may help maintain intermediate filament organization in intestinal epithelium. Phosphorylation of this protein may also influence mucin secretion in the small intestine. [provided by RefSeq, Dec 2015]

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