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Vps35 (BC005469) Mouse Untagged Clone
CAT＃: MC206802
Vps35 (untagged) - Mouse vacuolar protein sorting 35 (cDNA clone MGC:6633 IMAGE:3492842), (10ug)
CNY 2,000.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI647796; Mem3 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC005469
CGGACGCGTGGGCGGACGCGTGGGCGGCGGGGTAGGCGCTGCTGCTTGTGGTCCCCGGAGTCGCCATGCC TACAACACAGCAGTCACCCCAGGATGAGCAGGAAAAACTTTTGGATGAAGCCATCCAGGCTGTGAAGGTT CAGTCATTCCAGATGAAAAGATGCCTGGACAAAAACAAGCTGATGGATGCTCTGAAGCATGCCTCCAATA TGCTTGGAGAGCTCCGGACCTCTATGCTGTCACCAAAGAGTTACTATGAACTTTATATGGCTATTTCTGA TGAACTGCACTACTTGGAGGTCTACCTGACTGATGAATTTGCTAAAGGAAGAAAGGTGGCAGATCTCTAT GAACTTGTACAGTACGCGGGAAACATTATTCCAAGGCTTTATCTCTTGATCACAGTTGGAGTTGTATATG TCAAGTCATTTCCTCAATCCAGGAAAGATATTTTGAAAGATTTGGTAGAAATGTGCCGTGGTGTGCAGCA TCCGCTAAGGGGTTTGTTTCTTCGAAATTACCTTCTTCAGTGTACTAGGAACATTTTACCTGATGAAGGA GAGCCAACAGATGAAGAAACAACTGGTGATATCAGTGATTCCATGGATTTTGTACTACTCAACTTTGCAG AAATGAATAAGCTCTGGGTGCGGATGCAGCATCAAGGACATAGTCGAGATAGAGAAAAAAGAGAACGAGA GAGACAAGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTTAGTCAGTTGGAAGGTGTAAATGTG GAACGTTACAAACAGATTGTTCTAACAGGCATTCTGGAGCAGGTTGTGAATTGTAGGGATGCTCTGGCTC AGGAGTATCTCATGGAGTGCATCATTCAGGTTTTTCCTGATGAGTTTCATCTTCAGACTTTGAATCCTTT TCTTAGAGCTTGTGCTGAATTACACCAAAATGTAAATGTGAAAAACATAATCATTGCTTTAATTGACAGA TTAGCTTTATTTGCTCATCGTGAAGATGGACCCGGAATTCCAGCTGAGATTAAACTTTTTGACATATTTT CACAACAGGTGGCTACAGTGATACAGTCCAGACAAGACATGCCATCAGAGGATGTTGTATCTTTACAAGT CTCTCTCATTAATCTTGCTATGAAATGTTACCCTGATCGTGTGGACTACGTCGATAAAGTTCTAGAAACA ACAGTGGAGATATTCAATAAGCTTAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGAGCTTA CCAGACTTTTGAAGATACCTGTTGATACTTACAACAATATCTTAACAGTATTAAAGTTAAAGCATTTCCA CCCACTTTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGCTGTTATGTGCTTAGTAATGTTCTG GATTATAACACAGAAATCGTCTCTCAGGACCAGGTAGATTCCATAATGAATTTGGTGTCCACATTGATTC AGGATCAGCCAGACCAACCTGTAGAAGACCCTGATCCAGAAGACTTCGCTGATGAACAGAGCCTTGTTGG CCGATTTATTCATCTGCTACGTTCTGATGATCCTGACCAGCAGTATTTGATTTTGAATACGGCACGAAAA CATTTTGGGGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTACCAGT TGGCTTTTCGATACAAAGAGAATTCCCAAATGGATGACAAGTGGGAAAAGAAATGCCAGAAGATATTTTC ATTTGCTCACCAGACTATCAGTGCTTTGATTAAAGCTGAGCTGGCTGAATTACCACTGAGACTTTTTCTT CAAGGAGCATTAGCTGCTGGAGAAATTGGCTTTGAAAATCATGAAACAGTAGCATATGAATTTATGTCCC AGGCATTTTCTCTATATGAAGATGAAATCAGTGATTCTAAAGCACAGCTGGCTGCTATCACCTTGATCAT CGGTACTTTTGAGAGGATGAAATGCTTCAGTGAAGAGAATCATGAACCCTTGAGAACTCAGTGTGCACTT GCTGCATCAAAACTTCTGAAAAAACCAGATCAAGGCCGAGCCGTGAGCACATGTGCACATCTCTTTTGGT CTGGCCGAAACACAGACAAAAATGGGGAAGAGCTTCATGGAGGTAAAAGGGTCATGGAGTGCCTAAAGAA GGCACTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTTCAGCTCTTTATAGAGATTCTGAAC AGGTACATCTATTTCTATGAAAAAGAAAATGATGCGGTAACCATTCAAGTCTTGAACCAACTTATTCAAA AGATTCGAGAAGATCTCCCAAATCTTGAGTCCAGTGAAGAAACAGAACAAATAAACAAGCATTTTCACAA CACATTGGAGCACTTGCGCTCAAGACGGGAATCACCAGAGTCTGAGGGGCCAATCTATGAAGGTCTCATC CTTTAAAAGAAGACAGCTCAGCATACTCCTTTCTACACATCAGAAAGGGCTTAACTAGGTCTCCCTTCCA TAGATTGTGCCTTTCAGAAATGCTGAGGTAGGTTTCCCATTCCTTACCTGTGATGTGTTTACCCAGCACC TCCAGACACCTTCAGGACCTTAACTCACTTCATAATTGTTTAAGTCTGTCTGATCACCCAAGTAGCATGA CTGATCTGCTCTTTAAACTTCTGTGATCCGTGAAAATTGAAAGCAAAACCCACTTATTCTGGCTACTAAT GGAGCTCTCCTTTCTTATTTTTATTTTGATGAATTGTTGATTGTGTATTTTCTTCATTAATGAGGAATAC TAACCCAAACATACTTGTTTCTTCGTTCTCTGGTCCTATGTGAGATTACTTTAGAGGACAGGAGTACTGT GCGTGAAATTCAGCTTGGGCTTTCCCTGAATGCAAGATAAAGCCTATGTGTAATATATTCCCTACAACTA GAATATATTAATGCATGTTGAAGAATTTTAAAGCACCATGGTCAGAAGCAGAAGCTATATTTTGCATATT TGGACTCAGCTATCCATTAAGAACTATGCCATCATCTGGATACATCAAAGTAATTTGATTCTATAAAATA GAAATTTTGTGACTTAATGTAATTTATGTATAACCTCCACTATTGTAAATTTATGTGAAATGCATCACAA CTACAACTTTTTCATCAGTGGAATAATAAAGTTGCTGTTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC005469 |
| Insert Size | 2391 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC005469, AAH05469 |
| RefSeq Size | 3295 bp |
| RefSeq ORF | 2391 bp |
| Locus ID | 65114 |
| Gene Summary | Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of WASHC2 and mediates the association of the CSC with the WASH complex (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210707 | Vps35 (tGFP-tagged) - Mouse vacuolar protein sorting 35 (cDNA clone MGC:6633 IMAGE:3492842) |
CNY 10,344.00 |
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