S1pr3 (NM_010101) Mouse Untagged Clone

CAT#: MC207261

S1pr3 (untagged) - Mouse sphingosine-1-phosphate receptor 3 (S1pr3), (10ug)



  "NM_010101" in other vectors (4)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit polyclonal Anti-Sphingosine 1-Phosphate Receptor 3 (extracellular)
    • 50 ul

CNY 11,000.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI132464; Edg3; LP; Lpb3; S1; S1p3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC207261 representing NM_010101
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAACCACGCATGCGCAGGGCCACCAGCCAGTCTTGGGAAATGACACTCTCCGGGAACATTACGATT
ACGTGGGGAAACTGGCAGGCAGGCTGCGGGACCCCCCTGAGGGTGGCACCCTCATCACCACCATCCTCTT
CTTGGTCACCTGTAGCTTCATCGTCTTGGAGAACCTGATGGTTTTGATTGCCATCTGGAAAAACAATAAA
TTTCACAACCGCATGTACTTTTTCATCGGCAACTTGGCTCTCTGCGACCTGCTGGCCGGCATAGCATACA
AGGTCAATATTCTTATGTCCGGCAGGAAGACGTTCAGTCTGTCTCCAACAGTGTGGTTCCTCAGGGAGGG
CAGTATGTTCGTAGCCCTGGGCGCATCCACCTGCAGCTTACTGGCCATTGCCATTGAGCGACACCTGACC
ATGATCAAGATGAGGCCATATGATGCCAACAAGAAGCACCGCGTGTTCCTTCTGATTGGGATGTGCTGGC
TAATTGCCTTCTCGCTGGGTGCCCTGCCAATCCTGGGCTGGAACTGCCTGGAGAACTTTCCCGACTGCTC
TACCATCTTGCCCCTCTACTCCAAGAAATACATCGCCTTCCTCATCAGTATCTTCACCGCCATTCTGGTG
ACCATCGTCATCTTGTATGCGCGCATCTACTGCCTGGTCAAGTCCAGCAGCCGCAGGGTGGCCAACCACA
ACTCTGAGAGATCCATGGCCCTTCTGCGGACCGTAGTGATTGTGGTGAGTGTGTTCATTGCCTGTTGGTC
CCCACTTTTTATCCTCTTCCTCATCGACGTGGCCTGCAGGGCAAAGGAGTGCTCCATCCTCTTCAAGAGT
CAGTGGTTCATCATGCTGGCTGTCCTCAACTCGGCCATGAACCCTGTCATCTACACGCTGGCCAGCAAAG
AGATGAGGCGCGCCTTCTTCCGGTTGGTGTGCGGCTGTCTAGTCAAGGGCAAGGGGACCCAGGCCTCACC
CATGCAGCCTGCCCTCGACCCAAGCAGAAGTAAGTCAAGCTCTAGTAACAACAGCAGCCACTCTCCGAAG
GTCAAGGAAGACCTGCCCCGCGTGGCTACTTCTTCCTGCATCATTGACAAAAACAGGTCGTTTCAGAACG
GGGTCCTCTGCAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_010101
Insert Size 1137 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC068176, AAH68176
RefSeq Size 4484 bp
RefSeq ORF 1137 bp
Locus ID 13610
UniProt ID Q9Z0U9
Gene Summary This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for the lysophospholipid sphingosine 1-phosphate. The gene product functions in endothelial cells and is involved in vascular and heart development. The gene product mediates HDL and HDL-associated lysophospholipid-induced vasorelaxation, and it coordinates with other lysophospholipid receptors in the process of angiogenesis. [provided by RefSeq, Jan 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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