United States
Germany
Japan
United Kingdom
China
Got1 (NM_010324) Mouse Untagged Clone
CAT＃: MC207293
Got1 (untagged) - Mouse glutamate oxaloacetate transaminase 1, soluble (Got1), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI789014; cAspAT; cCAT; Got-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207293 representing NM_010324
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTCCATCAGTCTTTGCCCAGGTTCCGCAAGCTCCTCCGGTTCTGGTCTTTAAGCTCACTGCGG ACTTCCGGGATGATCCAGATCCCCGCAAGGTTAACCTCGGCGTGGGAGCGTACCGCACAGATGAATCTCA GCCCTGGGTTTTGCCAGTAGTGAGGAAGGTCGAACAGAAGATTGCTAACGACAACAGCCTCAACCACGAG TACCTGCCCATCCTGGGCCTGGCAGAGTTCCGGAGCTGTGCTTCTCGCCTAGTTCTTGGGGACAACAGCC TGGCTATCAGGGAGAATCGGGTTGGAGGGGTGCAGTCTTTGGGAGGGACAGGCGCTCTTCGGATTGGAGC TGACTTCTTAGGGCGATGGTACAATGGTACAGATAACAAGAACACACCAATCTACGTATCATCACCAACC TGGGAGAACCATAATGCTGTGTTTTCTGCCGCCGGTTTTAAGGACATTCGGCCCTATTGCTACTGGGATG CGGAGAAGAGAGGACTGGACCTCCAGGGTTTCCTGAATGATCTGGAGAATGCCCCCGAGTTCTCCATCTT TGTCCTCCATGCCTGTGCGCACAACCCAACAGGGACCGACCCGACTCCAGAGCAGTGGAAGCAGATCGCT GCTGTCATGCAGCGCCGTTTTCTGTTCCCCTTCTTTGACTCAGCCTATCAGGGCTTTGCATCTGGAGACC TAGAGAAAGATGCGTGGGCTATTCGCTATTTTGTGTCTGAAGGCTTCGAGCTCTTCTGTGCCCAGTCCTT CTCCAAGAACTTCGGGCTCTACAATGAGAGAGTGGGGAATCTGACCGTGGTCGGAAAAGAGTCTGACAGC GTCCTGCGGGTCCTTTCCCAGATGGAGAAGATTGTACGAATCACCTGGTCCAATCCCCCAGCCCAAGGAG CTCGGATTGTGGCCGCCACCCTCTCTGACCCGGAGCTCTTTAAGGAGTGGAAAGGTAACGTGAAGACAAT GGCTGACCGGATTCTGACCATGAGATCCGAACTCAGGGCAAGACTAGAAGCTCTCAAGACCCCCGGGACT TGGTCTCACATCACTGAGCAGATTGGAATGTTCAGTTTCACCGGCTTGAACCCCAAGCAGGTCGAGTATT TGGTCAACGAGAAGCATATCTATCTCCTGCCGAGTGGTCGGATCAACATGTGCGGCTTGACCACCAAGAA CCTAGATTACGTCGCTACCTCCATCCATGAAGCCGTCACCAAAATCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010324 |
| Insert Size | 1242 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010324.2, NP_034454.2 |
| RefSeq Size | 2065 bp |
| RefSeq ORF | 1242 bp |
| Locus ID | 14718 |
| UniProt ID | P05201 |
| Gene Summary | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206497 | Got1 (Myc-DDK-tagged) - Mouse glutamate oxaloacetate transaminase 1, soluble (Got1) |
CNY 5,488.00 |
|
| MR206497L1 | Lenti ORF clone of Got1 (Myc-DDK-tagged) - Mouse glutamate oxaloacetate transaminase 1, soluble (Got1) |
CNY 7,888.00 |
|
| MR206497L2 | Lenti ORF clone of Got1 (mGFP-tagged) - Mouse glutamate oxaloacetate transaminase 1, soluble (Got1) |
CNY 5,990.00 |
|
| MR206497L3 | Lenti ORF clone of Got1 (Myc-DDK-tagged) - Mouse glutamate oxaloacetate transaminase 1, soluble (Got1) |
CNY 5,990.00 |
|
| MR206497L4 | Lenti ORF clone of Got1 (mGFP-tagged) - Mouse glutamate oxaloacetate transaminase 1, soluble (Got1) |
CNY 5,990.00 |
