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Atat1 (NM_001142744) Mouse Untagged Clone
CAT＃: MC207731
Atat1 (untagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 1, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0610011P08Rik; 2610008K08Rik; 2610110G12Rik; 3110080J08Rik; Mec17; TAT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001142744 |
| Insert Size | 1266 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001142744.1, NP_001136216.1 |
| RefSeq Size | 1463 bp |
| RefSeq ORF | 1266 bp |
| Locus ID | 73242 |
| UniProt ID | Q8K341 |
| Gene Summary | Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). It should be noted that this variant contains an overlapping upstream ORF that may interfere with the translation of the annotated CDS. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206707 | Atat1 (Myc-DDK-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 1 |
CNY 3,656.00 |
|
| MR206707L3 | Lenti ORF clone of Atat1 (Myc-DDK-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 1 |
CNY 5,230.00 |
|
| MR206707L4 | Lenti ORF clone of Atat1 (mGFP-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 1 |
CNY 5,230.00 |
