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Spsb1 (NM_029035) Mouse Untagged Clone
CAT＃: MC207745
Spsb1 (untagged) - Mouse splA/ryanodine receptor domain and SOCS box containing 1 (Spsb1), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110014L01Rik; 4930422J18Rik; AI596360; AI854583; SSB-1; SSB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207745 representing NM_029035
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCAGAAGGTCACAGGAGGGATCAAGACTGTGGACATGCGGGACCCCACATACCGACCTCTGAAGC AGGAACTCCAGGGGCTGGATTACTGCAAGCCCACCCGGCTGGACCTGCTGCTCGACATGCCCCCTGTGTC CTACGATGTGCAGCTGCTCCACTCCTGGAACAATAACGACCGTTCGCTCAACGTCTTCGTGAAGGAAGAT GACAAGTTGATCTTTCACCGGCATCCGGTGGCCCAGAGCACGGACGCCATCAGGGGCAAAGTTGGGTACA CACGTGGACTGCATGTATGGCAGATCACATGGGCCATGAGGCAACGAGGCACGCATGCCGTGGTGGGGGT GGCCACAGCAGATGCCCCTTTGCACTCCGTTGGGTACACAACCCTTGTAGGAAATAACCATGAATCCTGG GGCTGGGACCTGGGGCGTAACCGTCTCTACCACGACGGCAAGAACCAGCCAAGTAAAACATACCCAGCCT TTCTGGAGCCGGACGAGACATTCATTGTCCCTGACTCCTTTCTCGTGGCCCTGGACATGGATGATGGGAC CTTAAGTTTCATCGTGGATGGACAGTACATGGGAGTGGCTTTCCGGGGACTCAAGGGTAAAAAGCTGTAT CCTGTAGTGAGTGCCGTCTGGGGCCACTGTGAGATCCGCATGCGCTACTTGAACGGACTTGATCCTGAGC CCCTGCCACTCATGGACCTGTGCCGGCGTTCGGTGCGCCTAGCGCTGGGAAAGGAGCGCCTGGGTGCCAT CCCCGCTCTGCCGCTACCTGCCTCCCTCAAAGCCTACCTCCTCTACCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_029035 |
| Insert Size | 822 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_029035.2, NP_083311.1 |
| RefSeq Size | 3089 bp |
| RefSeq ORF | 822 bp |
| Locus ID | 74646 |
| UniProt ID | Q9D5L7 |
| Gene Summary | Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (By similarity). Acts as a bridge which links the NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203651 | Spsb1 (tGFP-tagged) - Mouse splA/ryanodine receptor domain and SOCS box containing 1 (Spsb1) |
CNY 2,850.00 |
|
| MR203651 | Spsb1 (Myc-DDK-tagged) - Mouse splA/ryanodine receptor domain and SOCS box containing 1 (Spsb1) |
CNY 2,400.00 |
|
| MR203651L3 | Lenti ORF clone of Spsb1 (Myc-DDK-tagged) - Mouse splA/ryanodine receptor domain and SOCS box containing 1 (Spsb1) |
CNY 4,750.00 |
|
| MR203651L4 | Lenti ORF clone of Spsb1 (mGFP-tagged) - Mouse splA/ryanodine receptor domain and SOCS box containing 1 (Spsb1) |
CNY 4,750.00 |
