Htr1a (NM_008308) Mouse Untagged Clone

CAT#: MC208723

Htr1a (untagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a), (10ug)



  "NM_008308" in other vectors (4)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Goat Anti-Htr1a Antibody
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gpcr18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208723 representing NM_008308
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATATGTTCAGTCTTGGCCAGGGCAACAACACCACAACGTCCCTGGAGCCCTTCGGGACAGGCGGCA
ACGATACTGGCCTCTCCAACGTGACCTTCAGCTACCAAGTGATCACCTCTCTTTTGCTGGGGACGCTCAT
TTTCTGCGCGGTGCTCGGCAATGCCTGCGTGGTTGCTGCCATCGCCCTGGAGCGCTCCCTTCAGAATGTT
GCCAACTATCTCATCGGCTCCTTGGCGGTCACCGATCTCATGGTGTCAGTGCTGGTGCTGCCCATGGCTG
CTCTGTACCAGGTGCTCAACAAGTGGACTCTGGGTCAGGTCACCTGTGACCTGTTTATCGCCCTGGATGT
GCTGTGCTGCACTTCGTCCATCCTGCACCTGTGCGCCATCGCGCTAGACAGGTACTGGGCAATCACCGAC
CCTATAGACTACGTGAACAAGAGGACGCCCCGGCGCGCCGCTGCGCTGATCTCGCTCACTTGGCTCATTG
GCTTTCTCATCTCCATCCCGCCTATGCTGGGCTGGCGCACCCCGGAAGACCGCTCGAACCCCAACGAGTG
CACCATCAGCAAGGACCACGGGTACACCATCTACTCCACTTTCGGCGCTTTCTATATTCCGCTGCTGCTC
ATGCTGGTCCTCTATGGGCGCATCTTCAGAGCCGCGCGCTTCCGAATCCGCAAGACGGTCAAGAAGGTGG
AAAAGAAGGGAGCGGGCACCAGCTTCGGAACATCGTCGGCCCCGCCCCCCAAGAAGAGCCTGAATGGTCA
GCCAGGTAGTGGGGACTGCAGGCGCAGTGCTGAGAACAGGGCGGTGGGGACTCCGTGCGCTAATGGGGCG
GTGAGACAGGGTGAGGACGACGCCACCCTGGAGGTGATCGAGGTGCATCGAGTGGGCAACTCCAAAGGGC
ACCTTCCTCTGCCCAGCGAATCAGGAGCTACCTCCTATGTCCCCGCCTGCTTGGAGAGAAAAAATGAGCG
CACTGCTGAGGCAAAGCGCAAGATGGCCTTGGCCCGTGAGAGGAAGACAGTGAAGACACTGGGCATCATC
ATGGGCACCTTCATCCTCTGCTGGCTGCCCTTTTTCATTGTGGCCCTGGTCCTACCTTTCTGTGAGAGCA
GTTGCCACATGCCTGAGTTGTTGGGTGCCATAATTAACTGGCTAGGCTACTCCAACTCCCTGCTCAACCC
AGTTATTTATGCTTATTTCAACAAAGACTTTCAAAACGCTTTTAAGAAGATCATCAAGTGCAAGTTCTGC
CGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_008308
Insert Size 1266 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC138669, AAI38670
RefSeq Size 3410 bp
RefSeq ORF 1266 bp
Locus ID 15550
UniProt ID Q64264
Gene Summary G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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