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Prkacb (NM_001164200) Mouse Untagged Clone
CAT＃: MC209175
Prkacb (untagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 4, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CbPKA; Pkacb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209175 representing NM_001164200
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTCACAAAGAACTGTCCAGTGGCCAGCACAGTGGCACGCCCACCGCCCTCCAGAAGCTGGAGG GGTTCGCTAGCCGCCTGTTTCACAGGCACTCCAGAGGCACCGCACAAGAGCACAGGGCAGCTCTGGAAGA TGACGGCCTTCGGGCCTCTGAGCACACTGCCTCATGGGACAAATCAATGAAAGAGTTTCTAGCCAAAGCC AAAGAAGACTTTCTGAGGAAATGGGAGAACCCTCCCCCGAGTAATGCTGGGCTTGAGGATTTTGAGAGGA AGAAAACCCTCGGGACGGGTTCCTTTGGAAGAGTCATGTTGGTGAAGCATAAAGCCACTGAGCAGTACTA CGCCATGAAGATCTTAGACAAGCAGAAGGTTGTTAAGCTGAAGCAAATAGAGCACACTCTGAATGAGAAG AGAATCCTGCAGGCCGTGGAGTTCCCGTTCCTTGTGCGGCTGGAGTACTCTTTTAAGGATAATTCTAATT TATACATGGTTATGGAATACGTCCCTGGGGGAGAGATGTTCTCACATCTGAGAAGAATTGGAAGGTTCAG TGAGCCCCACGCCCGTTTCTATGCAGCCCAGATTGTGCTAACATTTGAGTACCTTCATTCCCTCGACCTC ATCTACAGAGATCTCAAGCCGGAAAACCTCTTAATTGACCACCAGGGTTACATCCAGGTCACAGATTTCG GGTTCGCCAAAAGAGTCAAGGGCAGGACATGGACATTGTGTGGCACCCCAGAGTACCTGGCCCCGGAGAT CATCCTCAGCAAGGGTTACAATAAGGCGGTGGACTGGTGGGCACTGGGCGTGCTGATCTATGAGATGGCT GCTGGCTACCCTCCATTCTTTGCTGACCAGCCAATTCAGATCTATGAGAAGATTGTCTCTGGAAAGGTCC GGTTCCCATCACACTTCAGCTCCGATCTCAAGGACCTTCTGCGGAACCTGCTGCAGGTGGATCTGACAAA GCGATTCGGGAACCTGAAGAACGGCGTGAGTGACATAAAGACCCACAAGTGGTTTGCCACAACTGACTGG ATTGCTATTTATCAGAGAAAGGTTGAGGCTCCATTCATACCAAAGTTCAGAGGCTCTGGCGATACCAGCA ACTTCGATGACTATGAAGAAGAAGAAATCCGTGTGTCTATAACAGAAAAATGTGGAAAGGAATTTTGTGA ATTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001164200 |
| Insert Size | 1197 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001164200.1, NP_001157672.1 |
| RefSeq Size | 4302 bp |
| RefSeq ORF | 1197 bp |
| Locus ID | 18749 |
| UniProt ID | P68181 |
| Gene Summary | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed:9368018). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternative exon at the 5' end compared to variant 1. The resulting isoform (4) has a distinct and longer N-terminus, as compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217190 | Prkacb (tGFP-tagged) - Mouse protein kinase cAMP dependent catalytic beta (Prkacb) transcript variant 4, (10ug) |
CNY 7,088.00 |
|
| MR217190 | Prkacb (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 4 |
CNY 5,488.00 |
|
| MR217190L3 | Lenti ORF clone of Prkacb (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 4 |
CNY 5,230.00 |
|
| MR217190L4 | Lenti ORF clone of Prkacb (mGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 4 |
CNY 5,230.00 |
