Polb (NM_011130) Mouse Untagged Clone

CAT#: MC209200

Polb (untagged) - Mouse polymerase (DNA directed), beta (Polb), (10ug)



  "NM_011130" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A430088C08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209200 representing NM_011130
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAAACGCAAGGCGCCGCAGGAGACCCTCAACGGCGGCATCACGGACATGCTCGTGGAACTCGCAA
ACTTTGAGAAGAACGTGAGCCAGGCGATCCACAAGTACAATGCGTACAGAAAAGCGGCATCTGTGATAGC
CAAGTACCCACACAAAATCAAGAGCGGAGCGGAAGCTAAGAAACTGCCAGGAGTAGGAACAAAAATTGCT
GAAAAGATTGATGAATTTTTAGCAACTGGAAAATTGCGTAAACTGGAAAAGATTCGTCAAGATGATACAA
GTTCATCCATCAATTTCCTGACTCGAGTTACTGGCATTGGACCATCTGCTGCAAGGAAGTTTGTAGATGA
AGGAATTAAGACATTAGAAGATCTCAGGAAAAATGAAGATAAACTGAACCATCATCAACGAATTGGGCTG
AAATATTTCGAGGACTTTGAAAAGAGAATTCCTCGTGAGGAAATGCTGCAGATGCAGGATATTGTACTTA
ATGAAATTAAAAAAGTGGACTCTGAGTACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGCGCAGAGTC
GAGTGGAGACATGGATGTCCTGCTGACCCACCCAAACTTCACGTCAGAATCCAGCAAACAGCCAAAGTTG
TTACATCGTGTTGTGGAACAGTTACAAAAAGTCCATTTCATTACAGATACTCTGTCAAAGGGTGAAACAA
AGTTCATGGGTGTTTGCCAGCTTCCCAGCGAGAAGGATGGAAAGGAATATCCACACAGGAGAATCGATAT
CAGGTTGATCCCCAAAGATCAGTACTACTGTGGTGTTCTCTACTTCACTGGGAGTGACATCTTTAATAAG
AACATGAGAGCGCACGCCCTGGAAAAGGGCTTCACAATCAATGAGTACACCATCCGCCCCCTGGGGGTCA
CTGGAGTCGCTGGGGAGCCCCTTCCTGTGGACAGTGAGCAGGACATTTTTGATTACATCCAGTGGCGCTA
CCGGGAGCCCAAGGATAGAAGTGAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_011130
Insert Size 1008 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011130.2, NP_035260.1
RefSeq Size 1227 bp
RefSeq ORF 1008 bp
Locus ID 18970
UniProt ID Q8K409
Gene Summary Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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