Atg10 (NM_025770) Mouse Untagged Clone

CAT#: MC210437

Atg10 (untagged) - Mouse autophagy-related 10 (yeast) (Atg10), (10ug)



  "NM_025770" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5330424L23Rik; 5430428K15Rik; Agp10; AI852123; APG10; Apg10l; Apg10p; Atg10l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC210437 representing NM_025770
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGATGAGTTCTTTGGAGAAAAAAGCTTCCAGCATTATTGTGCAGAATTCATCAGACATTCACAGC
AGATAGGCGATGGCTGGGAATGGAGAACAGCCAAGGAATGTTCTGATGGCTACATGTGCAAAACACAGTT
TCGAATAAAGAATGAGACCTTGACACCACATGCCAGTGTGCTCACATGTCTCCCTACAGAGGAGAATTTA
GAGCTACCAATGGATGATTCTGAAGTGACGAGACCTGCAGCGGTGGCAGAAGTGATTAAACATGAGTATC
ATGTCTTATACTCTTGTAGTTACCAAGTGCCGGTTCTTTACTTTAGGGCAAGCTTTTTAGATGGGAGACC
GTTAGCTCTGGAGGATATATGGGAAGGTGTTCATGAGTGCTATAAGCCGAGGCTGCTCCAGGGACCGTGG
GATACCATCACCCAGCAGGAACATCCAATACTTGGGCAACCATTTTTTGTTCTACATCCCTGCAAGACAA
ATGAATTCATGACTGCTGTGCTAAAGAATTCACAGAAAATCAATAGAAATGTCAACTATATCACATCATG
GCTGAGCCTCGTGGGGCCAGTGGTTGGTCTGAATTTACCTCTGAGCTATGCTAAAGCAACATCACAATCG
GAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_025770
Insert Size 636 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_025770.3, NP_080046.3
RefSeq Size 1609 bp
RefSeq ORF 636 bp
Locus ID 66795
UniProt ID Q8R1P4
Gene Summary E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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