Arhgap27 (NM_183288) Mouse Untagged Clone
CAT#: MC211080
Arhgap27 (untagged) - Mouse Rho GTPase activating protein 27 (Arhgap27), transcript variant 3, (10ug)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 2310069I04Rik; 5730442P18Rik; Gm21552; Sh3d20 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC211080 representing NM_183288
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGACGTCGAGGGGGACGTGTACGTGCTGGTGGAACACCCCTTCGAGTACACCGGCAAAGACG GGCGCCGCATCGCCATCCAACCGAACGAGCGTTACCGACTGCTGCGCCGCAGCACCGAGCACTGGTGGCA CGTGCGGCGGGAGCCCGGGGGTCGCCCCTTCTACCTCCCCGCGCAGTACGTACGCGAGCTGCCCGCGCTC GGCGACCCTGCCCCGGCCCCGCAGCCTTCCGTACCGCAGCAGCGCCCCGCAGTCCCGGAACCTCTGGCCT ACGACTACCGCTTCGTGAGCACCCCGGTGGGCGCCGATGGGTCCTCTGCCGAGCCCCGGGGCCGCGCCAG CTCCCTGTGTGGCCCTGCACGACAGCGCACCGGTGGCCAGCGCAACAGCCTGGCTCCGGGAGGGCCCGCC TGCCTTTACGTGCGGCCCGCGGCGCCTGTGCGACCGGCGCAGTCCCTGGATGACTTGGCGCGCGGCGGTA CCGCGCCCCCTGCCGGCCTCCTCGGCAGCGCGGGCCACTTCAAGGCCTCCAGCGTGGCGGGCTCCTGGGT TTGTCCCCGGCCCCTGGCGCCTAGCGACTCGGAGAATGTCTACGAGGCCATTCCGGACTTGCGCTGCCCT CCGCGGGCAGAGAGCCCAAAGCAGGTAGGTCGCTGCGAGCGAGGGGGCCGGAGGGAGGTCACAGTCCAGC AGAGTCTAGATGCTTTTTTTTTTTTTAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_183288 |
Insert Size | 732 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_183288.3, NP_899111.2 |
RefSeq Size | 2099 bp |
RefSeq ORF | 732 bp |
Locus ID | 544817 |
Gene Summary | Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks several 3' exons and contains an alternate 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is significantly shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS represents a short variant at the 5' end of a much longer Arhgap27 gene. The protein includes an SH3 domain. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG213224 | Arhgap27 (tGFP-tagged) - Mouse SH3 domain containing 20 (Sh3d20), (10ug) |
CNY 2,850.00 |
|
MR213224 | Arhgap27 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 27 (Arhgap27), transcript variant 3 |
CNY 2,400.00 |
|
MR213224L3 | Lenti ORF clone of Arhgap27 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 27 (Arhgap27), transcript variant 3 |
CNY 4,750.00 |
|
MR213224L4 | Lenti ORF clone of Arhgap27 (mGFP-tagged) - Mouse Rho GTPase activating protein 27 (Arhgap27), transcript variant 3 |
CNY 4,750.00 |
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