Hpf1 (NM_028299) Mouse Untagged Clone

CAT#: MC211346

2700029M09Rik (untagged) - Mouse RIKEN cDNA 2700029M09 gene (2700029M09Rik), (10ug)



  "NM_028299" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2700029M09Rik; C4orf27; C230006B22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211346 representing NM_028299
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGGCGGTGGCGGGAAGCGCCGGACGGCCGGGGCGGGACCGCAGTGTGAAAAGACAGTTGAGGTGA
AGAAGAGTAAGTTTTCTGAGGCTGATGTCTCCAGTGACCTTCGAAAAGAAGTGGAAAATCTTTATAAGCT
TTCACTGCCTGAAGATTTCTATCATTTCTGGAAGTTCTGTGAAGAGCTTGATCCTGAAAAGCCAGCTGAT
GCGCTTGCCACAAGCCTGGGTCTTCGGTTAGTGGGTCCTTATGATATCCTTGCTGGGAAACATAAAATGA
AGAAAAAACCCACAGGCTTGAACTGTAATCTTCACTGGAGGTTTTACTATGACCCTCCTGAGTTCCAGAC
CATTATTATTGGAGATAATAAAACTCAGTACCACATGGGGTATTTCAGGGATTCTCCTGATGAACTTCCT
GTATACGTGGGTACAAATGAAGCAAAGAAAAATTGTATAATTATTCAAAATGGAGACAATGTGTTTGCTG
CCATCAAATTATTTTTAATGAAAAAACTCAAAGAAGTAACAGATAGAAAGAAAATTAGCATTTTGAAAAA
CATAGATGAAAAACTCACAGAAGCAGCCAGAAAACTAGGATACTCACTGGAACAGAGAACCGTGAAGATG
AGACAGAGAGATAAGAAGGTTGTGACAAAGACTTTTCATGGTGCAGGCTTGGTTGTTCCAGTAGATAAAA
ATGATGTTGGTTACCGAGAGCTCCCTGAAACAGATGCTGACCTTAAGAGAATCTGCAAGGCAGTTGTCGA
CGCTGCAAGCGACGAGGAGAGACTGAAAGCATTCGCTCCCATTCAGGAGATGATGACTTTTGTGCAGTTT
GCTAATGATGAGTGTGATTATGGCATGGGGCTGGAATTAGGAATGGACCTCTTTTGCTATGGCTCTCATT
ATTTTCACAAAGTTGCTGGTCAGCTTTTACCTCTTGCGTATAATCTATTGAAGAGGGATCTGTTTGCAAA
AATTATTGAAGATCATCTGGCAAGCAGAAGTGAAGAGAACATAGACCAGCTTGCAGGATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_028299
Insert Size 1041 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028299.1, NP_082575.1
RefSeq Size 1210 bp
RefSeq ORF 1041 bp
Locus ID 72612
UniProt ID Q8CFE2
Gene Summary Acts as a cofactor for serine ADP-ribosylation by conferring serine specificity on PARP1 and PARP2: interacts with PARP1 and PARP2 and is able to change amino acid specificity toward serine. Promotes histone serine ADP-ribosylation in response to DNA damage, limiting DNA damage-induced PARP1 hyper-automodification, and ensuring genome stability. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. HPF1 also promotes tyrosine ADP-ribosylation, probably by conferring tyrosine specificity on PARP1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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