Calcoco2 (NM_001100177) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410154J16Rik; C77254; Ndp52; Ndp52l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC211781 representing NM_001100177 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGTGCCCCATACCTACCTTGCTGGAACATGGCAACTTCTCTCAGGTCCTGTTTAACAATGTGG AGAAGTTCTATGCTCCTAGAGGAGATATCATGTGCTATTACACCCTCACTGAAAAGTTCATCCCTCGACG CAAGGACTGGATTGGCATCTTTAAAGTAGGGTGGAAGACCACTCAGGAGTATTATACCTTCATGTGGGCT CCCTTGCCAAAAGACCAAAACAAGGATTCAGCCACACAGCAGGAAATCCAATTCAAAGCTTATTACCTTC CCAAGGATGTGGAGCGCTACCAGTTCTGCTATGTGGATGAAGATGGTTTAGTCCGGGGAACAAGTGTCCC TTTCCAGTTTTGTCCAGACCCTGACGAGGACATAATGGTTGTTATCAATAAGGAAAAGGTAGAAGAGATG GAACAGCTCAGTGAGGAGCTTTACCAACAAAACCAGGAGCTGAAAGACAAGTACGCTGACCTCCATGAGC AGCTACAGAGGAAGCAGGTGGCACTGGAAGCAACACAGAGGGTCAATAAGACCTTAGAACACAAAGTGGA AGAGAAGGCCTCCTGGGAGAAAGAGAAGGCCTCCTGGGAGGAAGAGAAGGCCTCCTGGGAGGAAGAGAAG GCCTCCTGGGAGGAAGAGAAGGCCTCCTGGGAGAAAGAGAAGGCCTCCTGGGAGGAAGAGAAGGCCTCCT GGGAGAAAGAGAAGGCCCCCTGGGAGGTAGAGAAGGCCCCCTGGAAGGAAGTGAAGGCCTATTGGTGGAA TGATCTGCACCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100177
Insert Size	786 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100177.1, NP_001093647.1
RefSeq Size	1409 bp
RefSeq ORF	786 bp
Locus ID	76815
Gene Summary	Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation (By similarity). Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy (By similarity). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (By similarity). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (By similarity). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.