Pag1 (NM_053182) Mouse Untagged Clone

CAT#: MC212008

Pag1 (untagged) - Mouse phosphoprotein associated with glycosphingolipid microdomains 1 (Pag1), transcript variant B, (10ug)



  "NM_053182" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Cbp; F730007C19Rik; Pag
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC212008 representing NM_053182
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCCTGCAGGAAGCGTACTGAGCAGTGGGCAGATGCAGATGCAGATGGTCCTGTGGGGAAGTCTGG
CTGCCGTGGCCATGTTCTTCCTCATCACCTTCCTCGTCCTCCTGTGTTCCACTTGCGACAGAGAGAAGAA
GCCACGGCAGCATAGCGGGGACCATGAGAACCTGATGAATGTGCCTTCCGACAAGGACATGTTCAGCCAT
TCGGCAACCAGCCTGACAACAGATGCACTGGCTAGCAGCGAACAGAACGGGGTGCTCACCAATGGCGACA
TTCTTTCAGAAGACAGCACGCTGACCTGCATGCAGCACTATGAGGAAGTCCAGACCTCGGCTTCCGATCT
CCTGGACTCCCAGGACAGCACAGGAAAGGCCAAGTGCCACCAGAGCCGGGAGCTGCCCAGGATCCCGCCT
GAGAACGCCGTGGACGAGATTCTCACAGCCAGGGCTGCAGACACGGAACTGGGGCCCGGAGTGGAGGGGC
CCTACGAGGTGCTCAAGGACAGCTCCTCCCAGGAGAACATGGTGGAGGACTGTCTGTATGAAACAGTGAA
GGAGATCAAGGAAGTGGCAGACAAAGGCCAGGGTGGCAAGTCCAAGTCCACTTCTGCCTTGAAGGAGCTT
CAGGGGGCTCCCATGGAGGGCAAGGCCGACTTTGCTGAATATGCCTCTGTGGACAGAAACAAAAAGTGCC
GCCACAGCGCGAACGCAGAGAGCATTCTGGGAACTTGCAGTGACCTGGACGAGGAAAGCCCACCTCCTGT
CCCGGTTAAGCTTCTGGATGAGAATGCCAACCTTCCCCAGGAGGGAGGAGGACAGGCAGAGGAGCAAGCT
GCAGAAGGGACCGGTGGACACAGCAAGAGATTTAGTTCCTTGTCATACAAGTCTCGGGAAGAAGACCCGA
CTCTTACAGAAGAGGAGATCTCAGCAATGTATTCCTCAGTGAATAAGCCTGGCCAGTCGGCACACAAACC
TGGACCATGTATGAAAGGGCCAGAATCTGCCTGCCACTCCATGAAGGGCCTCCCTCAGAGGTCGTCATCT
TCCTGCAATGACCTCTATGCCACTGTGAAAGACTTTGAGAAAACTCCCAACAGCATCAGCACACTGCCCC
CGGCAAGGAGACCCAGTGAGGAGCCAGAGCCTGACTATGAAGCCATACAGACTCTAAACAGAGAGGATGA
GAAGGTCCCTCTGGAGACCAATGGCCACCATGTCCCCAAGGAGAGTGACTACGAGAGCATTGGGGACTTG
CAGCAATGCAGAGATGTCACCAGGCTCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053182
Insert Size 1290 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053182.5, NP_444412.2
RefSeq Size 8197 bp
RefSeq ORF 1290 bp
Locus ID 94212
UniProt ID Q3U1F9
Gene Summary Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (B) differs in the 5' UTR compared to variant A. Both variants A and B encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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