Elmo1 (NM_198093) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330578D22Rik; C230095H21Rik; CED-12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC212310 representing NM_198093 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGTGGTGAAGGAGCAGGTTATGAGAGCGCTTACAACCAAGCCTAGTTCCCTGGACCAGTTCAAAA GCAAGCTGCAAAACCTGAGCTACACCGAGATCCTGAAAATCCGCCAGTCTGAGAGGATGAACCAGGAAGA TTTCCAGTCTCGCCCGATTTTGGAACTAAAGGAGAAGATCCAGCCAGAAATCTTAGAGCTGATTAAACAG CAGCGCCTGAACCGCCTTGTGGAAGGGACCTGCTTTAGGAAACTCAATGCTCGCCGGAGACAAGACAAGT TTTGGTATTGTCGGCTTTCACCAAATCACAAGGTCTTACATTATGGCGACTTGGAAGAGAGCCCCCAAGG AGAAGTGCCCCACGATTCCCTGCAGGACAAATTGCCGGTGGCAGATATCAAAGCAGTGGTGACGGGAAAG GACTGCCCTCATATGAAAGAGAAAGGTGCCCTTAAACAAAACAAGGAGGTGCTTGAACTTGCTTTCTCCA TCTTATACGACTCAAATTGCCAACTGAACTTCATTGCTCCTGATAAGCATGAGTACTGCATCTGGACAGA TGGGCTGAATGCACTGCTTGGGAAGGACATGATGAGTGACCTGACACGCAATGACCTGGACACCCTGCTG AGCATGGAGATCAAGCTTCGCCTGCTGGACCTGGAAAACATCCAGATCCCGGATGCACCTCCGCCTATCC CCAAGGAACCTAGCAACTATGACTTTGTCTATGACTGTAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_198093
Insert Size	744 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_198093.3, NP_932761.1
RefSeq Size	4114 bp
RefSeq ORF	744 bp
Locus ID	140580
UniProt ID	Q8BPU7
Gene Summary	Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) represents use of an alternate promoter and differs in the 5' UTR and 5' coding region, compared to variant 3. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 3.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.