Rho (NM_145383) Mouse Untagged Clone

CAT#: MC212573

Rho (untagged) - Mouse rhodopsin (Rho), (10ug)



  "NM_145383" in other vectors (4)

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Noerg1; Opn2; Ops; RP4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC212573 representing NM_145383
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGGCACAGAGGGCCCCAATTTTTATGTGCCCTTCTCCAACGTCACAGGCGTGGTGCGGAGCCCCT
TCGAGCAGCCGCAGTACTACCTGGCGGAACCATGGCAGTTCTCCATGCTGGCAGCGTACATGTTCCTGCT
CATCGTGCTGGGCTTCCCCATCAACTTCCTCACGCTCTACGTCACCGTACAGCACAAGAAGCTGCGCACA
CCCCTCAACTACATCCTGCTCAACTTGGCCGTGGCTGACCTCTTCATGGTCTTCGGAGGATTCACCACCA
CCCTCTACACATCACTCCATGGCTACTTCGTCTTTGGGCCCACAGGCTGTAATCTCGAGGGCTTCTTTGC
CACACTTGGAGGTGAAATCGCCCTGTGGTCCCTGGTGGTCCTGGCCATTGAGCGCTACGTGGTGGTCTGC
AAGCCGATGAGCAACTTCCGCTTCGGGGAGAATCACGCTATCATGGGTGTGGTCTTCACCTGGATCATGG
CGTTGGCCTGTGCTGCTCCCCCACTCGTTGGCTGGTCCAGGTACATCCCTGAGGGCATGCAATGTTCATG
CGGGATTGACTACTACACACTCAAGCCTGAGGTCAACAACGAATCCTTTGTCATCTACATGTTCGTGGTC
CACTTCACCATTCCTATGATCGTCATCTTCTTCTGCTATGGGCAGCTGGTCTTCACAGTCAAGGAGGCGG
CTGCCCAGCAGCAGGAGTCAGCCACCACTCAGAAGGCAGAGAAGGAAGTCACCCGCATGGTTATCATCAT
GGTCATCTTCTTCCTGATCTGCTGGCTTCCCTACGCCAGTGTGGCCTTCTACATCTTCACCCACCAGGGC
TCCAACTTCGGCCCCATCTTCATGACTCTGCCAGCTTTCTTTGCTAAGAGCTCTTCCATCTATAACCCGG
TCATCTACATCATGTTGAACAAGCAGTTCCGGAACTGTATGCTCACCACGCTGTGCTGCGGCAAGAATCC
ACTGGGAGATGACGACGCCTCTGCCACCGCTTCCAAGACGGAGACCAGCCAGGTGGCTCCAGCCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_145383
Insert Size 1047 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC013125, AAH13125
RefSeq Size 3249 bp
RefSeq ORF 1047 bp
Locus ID 212541
UniProt ID P15409
Gene Summary Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (PubMed:9020854). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:27353443).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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