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Lgmn (NM_011175) Mouse Untagged Clone
CAT＃: MC215719
Lgmn (untagged) - Mouse legumain (Lgmn), (10ug)
CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A; AEP; AI746452; AU022324; Pr; Prsc1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC215719 representing NM_011175
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTGGAGAGTGGCTGTGCTTCTCAGCCTGGTGCTGGGTGCTGGTGCCGTTCCCGTCGGTGTGGACG ATCCCGAGGATGGAGGCAAGCACTGGGTGGTGATTGTGGCGGGCTCCAATGGCTGGTATAATTACCGACA CCAGGCAGACGCATGCCACGCCTACCAGATCATCCACCGGAACGGGATTCCTGACGAGCAGATCATAGTG ATGATGTATGACGACATTGCCAACTCTGAAGAAAACCCTACCCCAGGTGTTGTGATCAACCGACCTAACG GCACAGATGTATACAAGGGAGTCCTGAAGGACTACACCGGAGAGGATGTGACTCCAGAGAATTTCCTCGC CGTGCTGAGAGGTGACGCAGAAGCTGTGAAGGGCAAAGGGTCTGGAAAAGTCTTGAAGAGTGGCCCCCGA GATCATGTCTTCATTTACTTCACCGACCACGGAGCCACCGGGATCCTGGTGTTTCCTAATGATGATCTTC ATGTCAAGGACCTGAATAAGACTATTCGCTACATGTATGAACACAAAATGTACCAGAAGATGGTGTTCTA CATTGAAGCTTGTGAGTCTGGCTCCATGATGAACCACCTGCCCGACGACATCAACGTTTATGCAACTACT GCGGCCAACCCCAAGGAGTCATCTTATGCCTGCTACTACGACGAGGAGAGGGGCACTTACCTGGGTGACT GGTACAGCGTCAACTGGATGGAAGACTCCGATGTGGAGGACCTGACCAAAGAGACCCTTCACAAGCAGTA CCACCTGGTCAAGTCCCACACCAACACCAGCCATGTCATGCAATATGGGAACAAATCTATCTCTACCATG AAAGTGATGCAGTTTCAGGGAATGAAGCACAGAGCCAGTTCCCCCATCTCCCTGCCTCCGGTCACACACC TTGACCTCACCCCCAGCCCTGACGTGCCCCTGACCATCTTGAAGAGGAAGCTGCTGAGAACCAACGACGT GAAGGAATCCCAGAATCTCATTGGGCAGATCCAGCAATTTCTGGATGCCAGGCACGTCATTGAGAAGTCT GTGCACAAGATCGTTTCCCTGCTGGCGGGATTTGGGGAAACTGCTGAGAGACATCTGTCAGAGAGGACCA TGCTCACAGCACATGACTGCTACCAGGAGGCTGTAACCCACTTCCGCACACACTGCTTTAACTGGCACTC TGTCACGTACGAGCATGCCTTGCGGTACTTGTATGTGCTGGCCAATCTCTGTGAGGCACCATATCCGATT GACAGGATAGAGATGGCCATGGACAAAGTGTGTCTTAGTCACTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011175 |
| Insert Size | 1308 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011175.2, NP_035305.1 |
| RefSeq Size | 1874 bp |
| RefSeq ORF | 1308 bp |
| Locus ID | 19141 |
| UniProt ID | O89017 |
| Gene Summary | This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis. [provided by RefSeq, Aug 2016] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221565 | Lgmn (tGFP-tagged) - Mouse legumain (Lgmn), (10ug) |
CNY 3,710.00 |
|
| MR221565 | Lgmn (Myc-DDK-tagged) - Mouse legumain (Lgmn) |
CNY 3,656.00 |
|
| MR221565L3 | Lenti ORF clone of Lgmn (Myc-DDK-tagged) - Mouse legumain (Lgmn) |
CNY 5,230.00 |
|
| MR221565L4 | Lenti ORF clone of Lgmn (mGFP-tagged) - Mouse legumain (Lgmn) |
CNY 6,056.00 |
