Krt27 (NM_010666) Mouse Untagged Clone

CAT#: MC215948

Krt27 (untagged) - Mouse keratin 27 (Krt27), (10ug)



  "NM_010666" in other vectors (4)

CNY 5,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CK-27; Krt1-c2; Krt1-c29; mIRS; mIRSa3.1; st; stpm
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC215948 representing NM_010666
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGTTCGCTTTTCTTCCGCATCCAGACGGCTGGGCTCTGTGAGGCTCTCCAGCGCGGGAGCAGCCT
TGGGGGCTGGAAACGCATGCGGAGTGCCGGGCATCGGAAGCGGCTTCTCCTGCGCCTTTGGGGGCAGTTC
ATTGGCAGGGGGGCTCGGTATGGGCGGTGCTTCTTGTGGGGCCTTCACAGCGAATGAGCACGGCCTCCTC
TCCGGCAATGAGAAGGTCACCATGCAGAACCTTAATGACCGCCTGGCCTCCTACCTGGAGAACGTGCAAG
CACTGGAGGAGGCCAATGCTGACCTGGAGCAGAAGATCAAGGACTGGTATGAAAAATTTGGACCTGGGTC
GTGCCGCGGTCTCGATCATGACTACAGTAGATACTTCCCCATCATTGATGACCTCAGGACCCAGATCATC
TCTGCAACTGCACACAACGCCAACATCATCCTGCAAAATGACAACGCAAGACTGACGGCGGATGACTTCA
GAATGAAGTATGAGAATGAACTGGCACTCCACCAGAGCGTCGATGCTGACATCAATGGCTTGCGGAGGGT
CCTGGATGAGCTGACCCTGTGCAGAACCGACCTTGAGGTCCAGCTGGAAACACTCAGTGAGGAGCTGGCA
TACCTCAAGAAGAACCACGAGGAGGAAATGCAGGCTCTGCAGTGCGCCGCTGGAGGCAACGTGAACGTGG
AGATGAACGCAGCCCCGGGCGTGGACCTCACCGTCCTGCTGAACAACATGAGAGCCGAGTACGAGGCCCT
GGCTGAGCAGAACCGTAGGGACGCCGAGGCCTGGTTCCAAGAGAAGAGCGCCTCCCTGCAGCAGCAGATT
TCAGACGATGCTGGAGCCACCACCTCAGCCAGGAACGAACTTACAGAGATGAAACGGACTCTCCAAACCC
TGGAGATAGAGCTACAGTCCCTCTTAGCCATGAAACACTCTCTGGAGTGCTCCCTGACGGAGACCGAGGG
CAACTACTGCACGCAGCTGGCTCAGATCCAGGCTCAGATCAGCGCCCTGGAGGAGCAGCTGCACCAAGTG
AGGACGGAGACGGAGGGCCAGAAGCTGGAATATGAGCAGCTGCTGAATGTCAAGGCCCACCTGGAGAAGG
AGATCGAGACCTACTGCCGCCTCATAGATGGAGACGAGGGCTCTTGTGTTAAGGCAAAAGGCCAAGGAAG
ACCCGGAAATCAGACAAAAGATTCGCCTAAAACTGCCATCGTTAAAACTGTCGTGGAAGAGTTAGACCCC
CGTGGCAAGGTTCTCTCCTCCAGAGTTCACACCCTGGAAGAGAAATCCACCAAAGTCAACAAGACCGAGC
AGAGGATCCCTTCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010666
Insert Size 1347 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010666.2, NP_034796.1
RefSeq Size 1570 bp
RefSeq ORF 1347 bp
Locus ID 16675
UniProt ID Q9Z320
Gene Summary This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The mouse type I keratin genes are clustered in a region of chromosome 11. The encoded protein is involved in the formation of intermediate filaments in the inner root sheath. [provided by RefSeq, Sep 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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