United States
Germany
Japan
United Kingdom
China
Pou3f2 (NM_008899) Mouse Untagged Clone
CAT＃: MC215961
Pou3f2 (untagged) - Mouse POU domain, class 3, transcription factor 2 (Pou3f2), (10ug)
CNY 5,800.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9430075J19Rik; A230098E07Rik; Brn-2; Brn2; oct-7; OTF-7; Otf7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC215961 representing NM_008899
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGCAGCGTCTAACCACTACAGCCTGCTCACCTCCAGCGCCTCCATCGTACATGCCGAGCCGC CTGGCGGCATGCAGCAGGGCGCAGGGGGCTACCGCGAGGCGCAGAGCCTGGTGCAGGGCGACTACGGCGC GCTGCAGAGCAACGGGCACCCGCTCAGCCACGCTCACCAGTGGATCACCGCGCTGTCCCACGGCGGCGGC GGCGGGGGCGGCGGCGGCGGTGGAGGAGGCGGGGGAGGCGGCGGGGGAGGCGGCGACGGCTCCCCGTGGT CCACCAGCCCCCTAGGCCAGCCGGACATCAAGCCCTCGGTGGTGGTACAGCAGGGTGGCCGAGGCGACGA GCTGCACGGGCCAGGAGCGCTGCAGCAACAGCATCAACAGCAACAGCAACAGCAGCAGCAGCAGCAGCAG CAGCAGCAGCAGCAACAGCAGCAGCAACAACAGCGACCGCCACATCTGGTGCACCACGCTGCCAACCACC ATCCCGGGCCCGGGGCATGGCGGAGTGCGGCGGCTGCAGCTCACCTCCCTCCCTCCATGGGAGCTTCCAA CGGCGGTTTGCTCTATTCGCAGCCGAGCTTCACGGTGAACGGCATGCTGGGCGCAGGAGGGCAGCCGGCT GGGCTGCACCACCACGGCCTGAGGGACGCCCACGATGAGCCACACCATGCAGACCACCACCCGCATCCGC ACTCTCACCCACACCAGCAACCGCCCCCGCCACCTCCCCCACAAGGCCCACCGGGCCACCCAGGCGCGCA CCACGACCCGCACTCGGACGAGGACACGCCGACCTCAGACGACCTGGAGCAGTTCGCCAAGCAATTCAAG CAGAGGCGGATCAAACTCGGATTTACTCAAGCAGACGTGGGGCTGGCGCTTGGCACCCTGTACGGCAACG TGTTCTCGCAGACCACCATCTGCAGGTTTGAGGCCCTGCAGCTGAGCTTCAAGAACATGTGCAAGCTGAA GCCTTTGTTGAACAAGTGGTTGGAAGAGGCAGACTCATCCTCGGGCAGCCCCACCAGCATAGACAAGATC GCAGCGCAAGGGCGCAAACGGAAAAAGCGGACCTCCATCGAGGTGAGCGTCAAGGGGGCTCTGGAGAGCC ATTTCCTCAAATGCCCTAAGCCCTCGGCCCAGGAGATCACCTCCCTCGCGGACAGCTTACAGCTGGAGAA GGAGGTGGTGAGAGTTTGGTTTTGTAACAGGAGACAGAAAGAGAAAAGGATGACCCCTCCCGGAGGGACT CTGCCGGGCGCCGAGGATGTGTATGGGGGTAGTAGGGACACGCCACCACACCACGGGGTGCAGACGCCCG TCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008899 |
| Insert Size | 1338 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008899.2, NP_032925.1 |
| RefSeq Size | 6272 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 18992 |
| UniProt ID | P31360 |
| Gene Summary | Transcription factor that plays a key role in neuronal differentiation (PubMed:24243019). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (PubMed:24243019).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nuc-ErbB3 regulates H3K27me3 levels and HMT activity to establish epigenetic repression during peripheral myelination
,Ness, JK;Skiles, AA;Yap, EH;Fajardo, EJ;Fiser, A;Tapinos, N;,
Glia
,PubMed ID 27017927
[Pou3f2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225266 | Pou3f2 (tGFP-tagged) - Mouse POU domain class 3 transcription factor 2 (Pou3f2), (10ug) |
CNY 4,370.00 |
|
| MR225266 | Pou3f2 (Myc-DDK-tagged) - Mouse POU domain, class 3, transcription factor 2 (Pou3f2) |
CNY 3,656.00 |
|
| MR225266L1 | Lenti ORF clone of Pou3f2 (Myc-DDK-tagged) - Mouse POU domain, class 3, transcription factor 2 (Pou3f2) |
CNY 6,056.00 |
|
| MR225266L2 | Lenti ORF clone of Pou3f2 (mGFP-tagged) - Mouse POU domain, class 3, transcription factor 2 (Pou3f2) |
CNY 5,890.00 |
|
| MR225266L3 | Lenti ORF clone of Pou3f2 (Myc-DDK-tagged) - Mouse POU domain, class 3, transcription factor 2 (Pou3f2) |
CNY 5,890.00 |
|
| MR225266L4 | Lenti ORF clone of Pou3f2 (mGFP-tagged) - Mouse POU domain, class 3, transcription factor 2 (Pou3f2) |
CNY 5,890.00 |
