Pou3f2 (NM_008899) Mouse Untagged Clone

CAT#: MC215961

Pou3f2 (untagged) - Mouse POU domain, class 3, transcription factor 2 (Pou3f2), (10ug)



  "NM_008899" in other vectors (6)

CNY 5,800.00


货期*
6周

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9430075J19Rik; A230098E07Rik; Brn-2; Brn2; oct-7; OTF-7; Otf7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC215961 representing NM_008899
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCGCAGCGTCTAACCACTACAGCCTGCTCACCTCCAGCGCCTCCATCGTACATGCCGAGCCGC
CTGGCGGCATGCAGCAGGGCGCAGGGGGCTACCGCGAGGCGCAGAGCCTGGTGCAGGGCGACTACGGCGC
GCTGCAGAGCAACGGGCACCCGCTCAGCCACGCTCACCAGTGGATCACCGCGCTGTCCCACGGCGGCGGC
GGCGGGGGCGGCGGCGGCGGTGGAGGAGGCGGGGGAGGCGGCGGGGGAGGCGGCGACGGCTCCCCGTGGT
CCACCAGCCCCCTAGGCCAGCCGGACATCAAGCCCTCGGTGGTGGTACAGCAGGGTGGCCGAGGCGACGA
GCTGCACGGGCCAGGAGCGCTGCAGCAACAGCATCAACAGCAACAGCAACAGCAGCAGCAGCAGCAGCAG
CAGCAGCAGCAGCAACAGCAGCAGCAACAACAGCGACCGCCACATCTGGTGCACCACGCTGCCAACCACC
ATCCCGGGCCCGGGGCATGGCGGAGTGCGGCGGCTGCAGCTCACCTCCCTCCCTCCATGGGAGCTTCCAA
CGGCGGTTTGCTCTATTCGCAGCCGAGCTTCACGGTGAACGGCATGCTGGGCGCAGGAGGGCAGCCGGCT
GGGCTGCACCACCACGGCCTGAGGGACGCCCACGATGAGCCACACCATGCAGACCACCACCCGCATCCGC
ACTCTCACCCACACCAGCAACCGCCCCCGCCACCTCCCCCACAAGGCCCACCGGGCCACCCAGGCGCGCA
CCACGACCCGCACTCGGACGAGGACACGCCGACCTCAGACGACCTGGAGCAGTTCGCCAAGCAATTCAAG
CAGAGGCGGATCAAACTCGGATTTACTCAAGCAGACGTGGGGCTGGCGCTTGGCACCCTGTACGGCAACG
TGTTCTCGCAGACCACCATCTGCAGGTTTGAGGCCCTGCAGCTGAGCTTCAAGAACATGTGCAAGCTGAA
GCCTTTGTTGAACAAGTGGTTGGAAGAGGCAGACTCATCCTCGGGCAGCCCCACCAGCATAGACAAGATC
GCAGCGCAAGGGCGCAAACGGAAAAAGCGGACCTCCATCGAGGTGAGCGTCAAGGGGGCTCTGGAGAGCC
ATTTCCTCAAATGCCCTAAGCCCTCGGCCCAGGAGATCACCTCCCTCGCGGACAGCTTACAGCTGGAGAA
GGAGGTGGTGAGAGTTTGGTTTTGTAACAGGAGACAGAAAGAGAAAAGGATGACCCCTCCCGGAGGGACT
CTGCCGGGCGCCGAGGATGTGTATGGGGGTAGTAGGGACACGCCACCACACCACGGGGTGCAGACGCCCG
TCCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_008899
Insert Size 1338 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008899.2, NP_032925.1
RefSeq Size 6272 bp
RefSeq ORF 1338 bp
Locus ID 18992
UniProt ID P31360
Gene Summary Transcription factor that plays a key role in neuronal differentiation (PubMed:24243019). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (PubMed:24243019).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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