Gsdma (NM_021347) Mouse Untagged Clone
CAT#: MC215991
Gsdma (untagged) - Mouse gasdermin A (Gsdma), (10ug)
CNY 5,800.00
Product images
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | BB149167; Gsdm; Gsdm1; Gsdma1; H312E |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC215991 representing NM_021347
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTATGTTTGAGAATGTCACCCGGGCCCTGGCTAGACAGCTGAACCCTCGAGGGGATCTGACACCCC TAGACAGCCTCATCGACTTCAAACGCTTCCATCCCTTCTGCCTGGTGCTGAGGAAGAGGAAGAGCACACT GTTCTGGGGAGCCCGCTATGTGCACACCGACTACACTCTCCTGGATGTGCTGGAGCCGGGCAGCTCCCCC TCAGATCCGACAGACAGTGGCAACTTTAGCTTTAAGAATATGCTGGATGCTCGAGTAGAGGGAGATGTGG ATGTGCCAAAGACAGTGAAGGTAAAGGGGACTGCGGGTCTGTCACGGAGCAGCACACTGGAGGTGCAGAC GCTCAGCGTGGCTCCCACGGCTCTGGAGAACTTGCACAAGGAGAGGAAACTGTCAGCAGACCACCCATTC CTGAAGGAGATGCGGGAACGCGGGGAGAACCTCTATGTGGTGATGGAGGTGGTGGAAACCCTACAGGAAG TCACTCTGGAGCGAGCCGGCAAGGCAGAGGGCTGCTTCTCTCTCCCCTTCTTTGCCCCACTGGGACTACA GGGATCCGTGAACCACAAGGAGGCTGTAACCATCCCCAAGGGCTGTGTTCTGGCCTATCGAGTGAGACAA CTGATGGTCAACGGCAAAGATGAGTGGGGCATTCCACACATTTGCAATGACAGCATGCAAACCTTCCCTC CTGGAGAAAAGCCAGGAGAAGGGAAGTTCATATTGATCCAGGCATCTGATGTTGGGGAGATGCACGAAGA CTTCAAGACATTAAAGGAAGAGGTTCAGCGAGAGACTCAGGAAGTGGAGAAGTTAAGTCCAGTGGGGCGA AGCTCACTACTCACTTCCCTCAGCCATCTCCTAGGAAAGAAGAAAGAGCTCCAGGACCTTGAGCAGACGC TTGAAGGGGCTCTAGACAAGGGACACGAAGTGACCCTGGAAGCACTCCCCAAAGATGTCCTGCTGTCAAA GGACGCTATGGACGCCATCCTTTACTTCCTCGGGGCTCTGACAGTGCTAAGTGAAGCCCAACAGAAGCTT CTAGTAAAATCCTTGGAGAAAAAGATCCTACCGGTGCAACTGAAGCTGGTTGAAAGCACCATGGAGAAGA ACTTCCTGCAAGATAAAGAGGGTGTTTTCCCCCTGCAACCTGATCTGCTCTCCTCCCTCGGGGAGGAGGA ACTGATCCTAACAGAAGCACTGGTGGGACTAAGCGGCCTGGAAGTCCAGAGATCAGGCCCCCAGTACACG TGGGATCCGGACACGCTCCCCCACCTTTGTGCCCTCTATGCTGGCCTCTCCCTCCTTCAACTGCTAAGCA AGAATTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_021347 |
Insert Size | 1341 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021347.4, NP_067322.1 |
RefSeq Size | 2726 bp |
RefSeq ORF | 1341 bp |
Locus ID | 57911 |
UniProt ID | Q9EST1 |
Gene Summary | May promote pyroptosis. Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity. The physiological relevance of these observations is unknown.[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG220293 | Gsdma (tGFP-tagged) - Mouse gasdermin A (Gsdma), (10ug) |
CNY 4,940.00 |
|
MR220293 | Gsdma (Myc-DDK-tagged) - Mouse gasdermin A (Gsdma) |
CNY 5,488.00 |
|
MR220293L3 | Lenti ORF clone of Gsdma (Myc-DDK-tagged) - Mouse gasdermin A (Gsdma) |
CNY 6,370.00 |
|
MR220293L4 | Lenti ORF clone of Gsdma (mGFP-tagged) - Mouse gasdermin A (Gsdma) |
CNY 6,370.00 |