Crbn (NM_175357) Mouse Untagged Clone

CAT#: MC215992

Crbn (untagged) - Mouse cereblon (Crbn), transcript variant 2, (10ug)



  "NM_175357" in other vectors (4)

CNY 3,656.00

CNY 5,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2610203G15Rik; 2900045O07Rik; AF229032; AW108261; piL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC215992 representing NM_175357
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCGAGGGAGATCAGCAGGACGCTGCGCACAACATGGGAAACCACCTGCCGCTTCTGCCTGCAG
ACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAGACCAAGATAGTAAAGAAGCCAGAAAACC
GAATATCATAAACTTTGACACCAGTCTGCCAACCTCACATACATACCTGGGAGCTGATATGGAGGAGTTC
CACGGGAGAACTTTGCATGACGACGACAGCTGCCAGGTGATCCCAGTCCTTCCTGAGGTGCTGATGATCC
TGATTCCTGGGCAGACACTCCCACTGCAGCTCTCTCACCCACAGGAAGTCAGCATGGTGCGGAACTTAAT
CCAGAAAGACAGGACCTTTGCAGTCCTTGCATACAGTAATGTGCAAGAAAGGGAAGCACAGTTTGGGACA
ACAGCAGAGATCTATGCCTATCGAGAAGAGCAGGAGTTTGGAATTGAAGTAGTGAAAGTGAAAGCAATTG
GAAGGCAGCGGTTCAAGGTCCTCGAACTTCGAACACAGTCAGATGGAATCCAGCAAGCTAAAGTGCAGAT
TTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTGCAGTTAGAATCACTCAATAAGTGCCAGGTA
TTTCCTTCAAAACCCATCTCCTGGGAAGACCAGTATTCATGTAAATGGTGGCAGAAATACCAGAAGAGAA
AGTTTCACTGTGCAAATCTAACATCATGGCCTCGCTGGCTGTATTCATTATATGATGCTGAAACATTAAT
GGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCTCAAAGATGATTCTCTTCCTGAAAATCCA
ATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGATGATGTATTGAGAATTCAGCTCCTTAAAA
TCGGCAGTGCTATTCAACGGCTTCGCTGTGAATTGGACATCATGAACAAATGTACTTCCCTTTGCTGTAA
ACAATGTCAAGAAACAGAAATAACGACAAAGAATGAAATATTTAGTTTATCCTTATGTGGTCCAATGGCA
GCATATGTGAATCCTCATGGATATGTACATGAGACACTGACTGTGTATAAAGCGTCCAACCTGAATCTGA
TAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATGCATGGACCATTGCCCAGTGCAAGATCTG
TGCAAGCCATATTGGATGGAAATTTACAGCCACAAAAAAAGACATGTCACCTCAAAAATTTTGGGGCTTA
ACTCGCTCTGCTCTGTTACCCACAATTCCAGAGACTGAAGATGAAATAAGTCCAGACAAAGTAATACTTT
GTTTATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_175357
Insert Size 1338 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175357.2, NP_780566.1
RefSeq Size 4054 bp
RefSeq ORF 1338 bp
Locus ID 58799
UniProt ID Q8C7D2
Gene Summary This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]
Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the coding region compared to variant 1. It encodes isoform 2 which is longer than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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