Gpr61 (NM_175470) Mouse Untagged Clone

CAT#: MC216046

Gpr61 (untagged) - Mouse G protein-coupled receptor 61 (Gpr61), (10ug)



  "NM_175470" in other vectors (4)

CNY 3,656.00

CNY 5,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
GPR61 Antibody - N-terminal region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216046 representing NM_175470
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCTCACCCATCCCCCAGTCATCAGGAAACTCGTCCACTTTGGGAAGGGCCCTTCAAACCCCAG
GTCCCTCTACTGCCAGCGGGGTCCCAGAGTTGGGATTACGGGACGTGGCTTCAGAATCTGTGGCCCTCTT
CTTCATGCTCCTGTTGGATCTCACTGCTGTGGCTGGCAATGCTGCTGTGATGGCTGTTATTGCCAAGACA
CCCGCCCTCCGAAAATTTGTTTTTGTCTTCCATCTTTGTCTGGTGGACCTGCTGGCTGCCCTGACCCTCA
TGCCGCTTGCCATGCTCTCCAGCTCTGCCCTCTTTGACCACGCCCTCTTTGGGGAGGTGGCCTGCCGCCT
CTACCTGTTCCTGAGCGTTTGCTTTGTCAGCCTGGCCATCCTTTCGGTGTCTGCCATTAATGTGGAGCGC
TACTATTATGTGGTCCACCCAATGCGCTACGAGGTGCGCATGACACTAGGGCTGGTGGCCTCCGTGCTGG
TGGGCGTGTGGGTAAAGGCCCTAGCCATGGCTTCTGTGCCAGTGTTGGGAAGGGTCTACTGGGAGGAAGG
AGCTCCCAGTGTTAACCCCGGCTGTTCTCTCCAATGGAGCCATAGTGCCTACTGCCAGCTTTTTGTGGTG
GTCTTTGCTGTTCTGTACTTCTTGCTGCCCTTGATCCTGATCTTTGTGGTCTACTGCAGCATGTTTCGAG
TGGCTCGCGTGGCTGCCATGCAACACGGGCCGCTGCCCACGTGGATGGAGACGCCCCGGCAACGCTCTGA
GTCTCTCAGTAGCCGCTCTACTATGGTTACCAGCTCCGGGGCTCACCAGACCACCCCACACCGGACGTTT
GGGGGTGGGAAGGCAGCAGTGGTCCTCCTGGCTGTAGGGGGACAGTTCTTGCTTTGTTGGTTGCCCTACT
TCTCTTTCCATCTCTATGTTGCCCTGAGCGCACAGCCCATTTCAGCAGGACAGGTGGAGAACGTGGTAAC
CTGGATTGGCTACTTTTGCTTCACTTCCAACCCCTTTTTCTACGGATGTCTCAACCGTCAGATCCGGGGC
GAGCTTAGCAAACAGTTTGTCTGCTTCTTCAAGGCAGCTCCAGAGGAGGAGCTGAGGCTGCCTAGTCGTG
AGGGCTCCATTGAGGAGAATTTCCTGCAGTTCCTCCAGGGGACCTCTGAGAACTGGGTTTCTCGGCCCCT
ACCCAGTCCTAAGCGGGAGCCACCCCCTGTTGTTGACTTTCGAATCCCAGGCCAGATTGCTGAGGAGACC
TCAGAGTTCCTGGAGCAGCAACTCACCAGCGACATCATCATGTCCGACAGCTACCTCCGTCCCGCCCCCT
CACCAAGGCTGGAGTCATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_175470
Insert Size 1350 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175470.4, NP_780679.2
RefSeq Size 3686 bp
RefSeq ORF 1350 bp
Locus ID 229714
Gene Summary Orphan G-protein coupled receptor. Constitutively activates the G(s)-alpha/cAMP signaling pathway (By similarity). Shows a reciprocal regulatory interaction with the melatonin receptor MTNR1B most likely through receptor heteromerization (By similarity). May be involved in the regulation of food intake and body weight (PubMed:21971119).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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