Pold3 (NM_133692) Mouse Untagged Clone

CAT#: MC216088

Pold3 (untagged) - Mouse polymerase (DNA-directed), delta 3, accessory subunit (Pold3), (10ug)



  "NM_133692" in other vectors (4)

CNY 5,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2410142G14Rik; C85233; P66; P68
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216088 representing NM_133692
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAACAGCTGTATCTAGAAAACATAGACGAGTTCGTCACGGACCAGAACAAGATCGTGACTTACA
AGTGGCTAAGCTATACACTAGGGGTTCATGTTAACCAGGCCAAACAGATGCTCTATGAATATGTTGAAAG
GAAACGGAAAGAAAATTCGGGAGCTCAGCTGCATGTTACCTACTTGGTGTCTGGCAGTCTTATACAGAAC
GGACATTCTTGCCACAAGGTTGCAGTAGTGAGAGAAGATAAACTGGAAGCAGTGAAGTCCAAGTTAGCTG
TGACTGCCAGCATCCATGTGTACAGCATCCAGAAAGCTATGCTAAAGGACAGTGGGCCTCTGTTCAATAC
CGACTATGACATCCTTAAAAGCAATTTGCAGAACTGCAGCAAGTTTAGTGCCATACAGTGTGCAGCTGCA
GTCCCCAGAGCTCCTGCAGAATCCCCATCTTCCAGAAAGTATGAACAGTCAAATCTTCAGGCAGCGAGTG
AGGCACAAGCCAGTGAGCTGACTACCAATGGCCATGGTCCACCTGCCTCCAAACAGGCTTCCCAGCAGCC
CAAAGGAATTATGGGAATGTTAATCTCTAAAGCTGCTACTAAAACCCAGGACACCAACAAGGAAACCAAA
CCAGAGGCCCGAGAAGTAACCAGTGCATCTTCTGCTGGGGGCAAAGCACCAGGAAAAGGGAGTGTGATGA
GCAACTTTTTTGGAAAAGCTGCAATGAATAAACTTAAAGTCAATTTGGATTCAGAACAAGCAGTAAAGGA
AGAAAAAACAGTGGAGCAACCTCCAGTGTCTGTCACTGAACCAAAGCTGGCAGCTCCCCCAGCTCAGAAG
AAATCCAGCAGAAAGTCCGAGCCTGGGAAGGTGCAGCAGAAGGAGAAAAGCAGGGGCAAGCGAGTAGACT
TGTCGGATGAGGAGGCAAAGGAAACCGAACACCTGAAGAAAAAGAGAAGAAGAATCAAGCTTCCTCAGTC
TGATAGCAGTGAAGATGAAGTCTTCGAAGACTCCCCTGAGATGTATGAAGCAGACTCACCATCTCCACCT
CCTGTATCTCCACCTCCTGATCCTATGCCAAAAACTGAGCCCCCTCCTGTCAAGCGTTCAAGTGGAGAAA
CCAAAAGGAGACGAAAGCGTGTACTGAAATCTAAAACCTTTGTGGATGAAGAAGGCTGCATAGTGACTGA
GAAAGTCTATGAGAGTGAGTCCTGCACAGACAGTGAGGAGGAGCTTAAGATGAAGCCGGCCTCAGCACAC
AAACCCCCTGCTGCCGCTGTGAAAAGGGAGCCCAGAGAAGAACGGAAGGGCCCCAAGAAAGGGGCTGCTG
CTCTGGGCAAAGCCAACAGACAAGTGTCCATTACTGGCTTCTTCCAGAAAAAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133692
Insert Size 1386 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133692.2, NP_598453.1
RefSeq Size 3002 bp
RefSeq ORF 1386 bp
Locus ID 67967
Gene Summary As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:10219083, PubMed:27524497). Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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