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Gramd1c (NM_001172107) Mouse Untagged Clone
CAT＃: MC216184
Gramd1c (untagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 2, (10ug)
CNY 5,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4921521N14Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216184 representing NM_001172107
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACACTTGTTATCAGTTGAAGAAAATGTGCAGCCAAGAAGTCCAGGAAGAAGCAGCGTGGATGACG CTGGGGAAAGAGATGAGAAGTTCTCCAAGGCCGTCAGCTTTACACAGGAGTCAGTTAGCAGAGCTTCAGA AACAGAGCCATTGGACGGGAACTCACCGAAAAGAGGACTAGGAAAAGAGGATTCCCAGAGCGAGAGAAAT GTGAGAAAAAGTCCTTCGCTAGCTTCAGAAAAGAGGATAAGCAGAGCACCCTCCAAGTCACTGGACTTGA ATAAGAATGAGTACCTTTCTCTGGATAAAAGCAGCACTTCAGATTCTGTGGACGAAGAAAATATTCCCGA GAAAGATCTTCAAGGAAGACTTTATATCAACCGTGTCTTTCACATCAGTGCTGAGAGAATGTTCGAACTG CTGTTCACTAGCTCACACTTTATGCAGAGATTTGCAAATTCTAGAAATATAATAGATGTGGTATCTACCC CCTGGACAGTTGAATCTGGAGGCAATCAGCTGCGAACCATGACCTATACCATAGTCCTCAGCAACCCGCT AACTGGGAAGTATACTGCTGCCACAGAAAAGCAGACCCTGTATAAAGAGAGCCGGGAAGCACAGTTCTAC CTGGTAGACTCCGAAGTGCTGACACATGATGTGCCCTATCACGACTACTTCTACACTTTGAACAGATACT GTATCGTGAGATCTGCAAAACAGAGATGCAGGCTGAGAGTCTCCACAGACTTGAAGTACAGGAAACAACC ATGGGGCCTTATCAAGTCTTTAATTGAGAAGAATTCCTGGAGTTCACTGGAGAGCTACTTCAAAAAGCTT GAATCCGATTTGTTAATGGAAGAGTCTGTGTTGAGTCAATCCATTGAAGATGCTGGAAAACATAGCAGCC TACGCCGGAGAAGGCGGACCTTGAACCGGACAGCAGAGCCCGTTCCAAAGCTGTCCTCTCAGCGCTCTTC CACAGATTTGGGCTTCGAGGCCAAAGTAGATGTTACAGGAAAGAGAAAGACCGTGGACAGTTATGACACC GCCCTTATTGTGGTGATGAGCATATTCTTGCTGCTGCTCGTTCTGCTGAATGTGACACTATTTCTGAAGC TGTCAAAGATAGAACACGCTACCCAGTCCTTCTACCAGCTTCACCTCCAGGGAGAAAAATCTTTAAATTT AGTCTCTGACAGGTTCTCAAGAACAGAAAATATTCAAAAGAACAAAGATCAGGCCCACCGCCTAAAAGGA GTACTCCAAGATTCCATAGTGATGTTGGAACAGCTGAAGAGCTCACTCATTATGCTTCAAAAAACCTTTG ATTTACTAAATAAGAACAAGTCTGGGGTGGCTGTGGAGAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001172107 |
| Insert Size | 1374 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172107.1, NP_001165578.1 |
| RefSeq Size | 3276 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 207798 |
| UniProt ID | Q8CI52 |
| Gene Summary | Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216904 | Gramd1c (tGFP-tagged) - Mouse GRAM domain containing 1C (Gramd1c) transcript variant 2, (10ug) |
CNY 4,470.00 |
|
| MR216904 | Gramd1c (Myc-DDK-tagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 2 |
CNY 3,656.00 |
|
| MR216904L3 | Lenti ORF clone of Gramd1c (Myc-DDK-tagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 2 |
CNY 5,990.00 |
|
| MR216904L4 | Lenti ORF clone of Gramd1c (mGFP-tagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 2 |
CNY 5,990.00 |
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