United States
Germany
Japan
United Kingdom
China
Yap1 (NM_009534) Mouse Untagged Clone
CAT＃: MC216427
Yap1 (untagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 2, (10ug)
CNY 3,656.00
CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI325207; Y; Yap; Yap65; Yk; Yki; yor; Yorkie |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216427 representing NM_009534
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCGCAACAGCCGCCGCCCCAGCCGGCCCCGCAAGGCCCCGCGCCGCCGTCCGTGTCTCCGG CCGGGACCCCCGCGGCCCCGCCCGCACCCCCGGCCGGCCACCAGGTCGTGCACGTCCGCGGGGACTCGGA GACCGACTTGGAGGCGCTCTTCAATGCCGTCATGAACCCCAAGACGGCCAACGTGCCTCAGACCGTGCCC ATGCGGCTTCGCAAGCTGCCCGACTCCTTCTTCAAGCCGCCTGAGCCCAAGTCCCACTCGCGACAGGCCA GTACTGATGCAGGTACTGCGGGAGCTCTGACTCCACAGCATGTTCGAGCTCACTCCTCTCCAGCCTCCCT GCAGCTGGGTGCCGTTTCTCCTGGGACACTCACAGCCAGTGGCGTTGTCTCTGGCCCTGCCGCTGCCCCT GCAGCTCAGCATCTCCGGCAGTCCTCCTTTGAGATCCCTGATGATGTACCACTGCCAGCAGGCTGGGAGA TGGCCAAGACATCTTCTGGTCAAAGATACTTCTTAAATCACAACGATCAGACAACAACATGGCAGGACCC CCGGAAGGCCATGCTTTCGCAACTGAACGTTCCTGCGCCTGCCAGCCCAGCGGTGCCCCAGACGCTGATG AATTCTGCCTCAGGACCTCTTCCTGATGGATGGGAGCAAGCCATGACTCAGGATGGAGAAGTTTACTACA TAAACCATAAGAACAAGACCACATCCTGGCTGGACCCAAGGCTGGACCCTCGTTTTGCCATGAACCAGAG GATCACTCAGAGTGCTCCAGTGAAGCAGCCCCCACCCTTGGCTCCCCAGAGCCCACAGGGAGGCGTCCTG GGTGGAGGCAGTTCCAACCAGCAGCAGCAAATACAGCTGCAGCAGTTACAGATGGAGAAGGAGAGACTGC GGTTGAAACAACAGGAATTATTTCGGCAGGAATTAGCTCTGCGCAGCCAGTTGCCTACACTGGAGCAGGA TGGAGGGACTCCGAATGCAGTGTCTTCTCCTGGGATGTCTCAGGAATTGAGAACAATGACAACCAATAGT TCCGATCCCTTTCTTAACAGTGGCACCTATCACTCTCGAGATGAGAGCACAGACAGCGGCCTCAGCATGA GCAGCTACAGCATCCCTCGGACCCCAGACGACTTCCTCAACAGTGTGGATGAAATGGATACAGGAGACAC CATCAGCCAAAGCACCCTGCCGTCACAGCAGAGCCGCTTCCCCGACTACCTGGAAGCCCTCCCTGGGACA AATGTGGACCTTGGCACACTGGAAGGAGATGCAATGAACATAGAAGGGGAGGAGCTGATGCCCAGTCTGC AGGAAGCGCTGAGTTCCGAAATCTTGGACGTGGAGTCTGTGTTGGCTGCCACCAAGCTAGATAAAGAAAG CTTTCTCACGTGGTTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009534 |
| Insert Size | 1419 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009534.2, NP_033560.1 |
| RefSeq Size | 4115 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 22601 |
| UniProt ID | P46938 |
| Gene Summary | This gene encodes a protein which binds to the SH3 domain of the Yes proto-oncogene product, a tyrosine kinase. This protein contains a WW domain, consisting of four conserved aromatic amino acids including two tryptophan residues. This conserved WW domain is found in various structural, regulatory and signaling molecules in various species, and may play a role in protein-protein interaction. Following cellular damage, phosphorylation of this encoded protein may suppress apoptosis. This protein may be involved in malignant transformation in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR212130 | Yap1 (Myc-DDK-tagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 2 |
CNY 3,656.00 |
|
| MR212130L3 | Lenti ORF clone of Yap1 (Myc-DDK-tagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 2 |
CNY 6,650.00 |
|
| MR212130L4 | Lenti ORF clone of Yap1 (mGFP-tagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 2 |
CNY 6,650.00 |
